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Properties of the NAD(P)H-dependent xylose reductase from the xylose-fermenting yeast Pichia stipitis
Open accessAbstract
Xylose reductase from the xylose-fermenting yeast Pichia stipitis was purified to electrophoretic and spectral homogeneity via ion-exchange, affinity and high-performance gel chromatography. The enzyme was active with various aldose substrates, such as DL-glyceraldehyde, L-arabinose, D-xylose, D-ribose, D-galactose and D-glucose. Hence the xylose reductase of Pichia stipitis is an aldose reductase (EC 1.1.1.21). Unlike all aldose reductases characterized so far, the enzyme from this yeast was active with both NADPH and NADH as coenzyme. The activity with NADH was approx. 70% of that with NADPH for the various aldose substrates. NADP+ was a potent inhibitor of both the NADPH- and NADH-linked xylose reduction, whereas NAD+ showed strong inhibition only with the NADH-linked reaction. These results are discussed in the context of the possible use of Pichia stipitis and similar yeasts for the anaerobic conversion of xylose into ethanol.
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Citations
Cited by
Redox balances in the metabolism of sugars by yeasts
VANDIJKEN, J., & SCHEFFERS, W. (1986) · FEMS Microbiology Letters · doi:10.1016/0378-1097(86)90291-0 · Cited by 641
Self-citationMetabolic engineering for improved fermentation of pentoses by yeasts
Jeffries, T. W., & Jin, Y.-S. (2004) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-003-1450-0 · Cited by 499
(2006) · doi:10.1007/978-3-540-28398-0 · Cited by 303
Evolution in Reverse: Engineering a D‐Xylose‐Specific Xylose Reductase
Nair, N. U., & Zhao, H. (2008) · ChemBioChem · doi:10.1002/cbic.200700765 · Cited by 52
Metadata
- Authors
- Verduyn, C., Van Kleef, R., Frank, J., Schreuder, H., Van Dijken, J. P., & Scheffers, W. A.
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1985-03-15
- Volume
- 226
- Issue
- 3
- Pages
- 669-677
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- OpenAlex
- W1855933245
- Cited by
- 301
- References
- 0
- FWCI
- 3.17
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Verduyn, C., Van Kleef, R., Frank, J., Schreuder, H., Van Dijken, J. P., & Scheffers, W. A. (1985). Properties of the NAD(P)H-dependent xylose reductase from the xylose-fermenting yeast Pichia stipitis. Biochemical Journal. https://doi.org/10.1042/bj2260669
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