Effects of dexamethasone treatment on insulin-stimulated rates of glycolysis and glycogen synthesis in isolated incubated skeletal muscles of the rat
Open accessAbstract
1. Rats were treated with dexamethasone for 4 days before measurement of the rates of lactate formation [which is an index of hexose transport; see Challiss, Lozeman, Leighton & Newsholme (1986) Biochem. J. 233, 377-381] and glycogen synthesis in response to various concentrations of insulin in isolated incubated soleus and extensor digitorum longus muscle preparations. 2. The concentration of insulin required to stimulate these processes half-maximally in soleus and extensor digitorum longus muscles isolated from control rats was about 100 muunits/ml. 3. Dexamethasone increases the concentration of insulin required to stimulate glycolysis half-maximally in soleus and extensor digitorum longus preparations to 250 and 300 muunits/ml respectively. The respective insulin concentrations necessary to stimulate glycogen synthesis half-maximally were about 430 and 370 muunits/ml for soleus and extensor digitorum longus muscle preparations isolated from steroid-treated rats. 5. Dexamethasone treatment did not change the amount of insulin bound to soleus muscle.
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Dexamethasone-induced insulin resistance does not alter tyrosine phosphorylation of the insulin receptor.
“Dexamethasone-induced insulin resistance does not alter tyrosine phosphorylation of the insulin receptor [18,26]”
Dexamethasone treatment inhibits insulin-stimulated glycogen synthesis and glycogen synthase activation.
“Dexamethasone treatment, in addition, inhibits insulin-stimulated glycogen synthesis and glycogen synthase activation [18,19].”
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METABOLISME ENERGETIQUE MITOCHONDRIAL DANS DES SITUATIONS DE PERTE DE POIDS
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- Authors
- Leighton, B., Challiss, R. A. J., Lozeman, F. J., & Newsholme, E. A.
- Affiliations
- University of Oxford (GB)
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1987-09-01
- Volume
- 246
- Issue
- 2
- Pages
- 551-554
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1148309
- OpenAlex
- W2416200405
- Cited by
- 31
- References
- 23
- Citations by year
- 2025: 1 · 2020: 1 · 2017: 1
- FWCI
- 1.38
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- dexamethasone, insulin, skeletal muscle, glycolysis, glycogen synthesis, insulin sensitivity, soleus muscle, extensor digitorum longus, hexose transport, lactic acid production, glucocorticoids, insulin binding
- MeSH terms
- Animals, Dexamethasone, Glycogen, Glycolysis, Insulin, Male, Muscles, Rats, Inbred Strains, Rats
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