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Epitope mapping of monoclonal antibodies to the paired helical filaments of Alzheimer's disease: identification of phosphorylation sites in tau protein
Open accessAbstract
Tau is a neuronal phosphoprotein the expression of which is developmentally regulated. A single tau isoform is expressed in fetal human brain but six isoforms are expressed in adult human brain, with the fetal isoform corresponding to the shortest adult isoform. Phosphorylation is also developmentally regulated, as fetal tau is phosphorylated at more sites than adult tau. In Alzheimer's disease, the six adult tau isoforms become hyperphosphorylated and form the paired helical filament (PHF), the major fibrous component of the neurofibrillary lesions. One way to identify phosphorylated sites in tau is to use antibodies that recognize phosphorylated residues within a specific amino acid sequence. We here characterize the two novel phosphorylation-dependent anti-tau antibodies AT270 and AT180 and identify their epitopes as containing phosphorylated Thr-181 and Thr-231 respectively. With these antibodies we show that these two threonine residues are partially phosphorylated in fetal and adult tau and almost fully phosphorylated in PHF tau. This result contrasts with previous studies of Ser-202 and Ser-396 which are partially phosphorylated in fetal tau, unphosphorylated in adult tau but almost fully phosphorylated in PHF tau.
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“No significant differences in phospho-tau levels relative to controls were observed (Supplementary Material, Fig. S4).”
From Functional genomic screen and network analysis reveal novel modifiers of tauopathy dissociated from tau phosphorylation (2011) · cites this paper as [86]
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Metadata
- Authors
- Goedert, M., Jakes, R., Crowther, R. A., Cohen, P., Vanmechelen, E., Vandermeeren, M., & Cras, P.
- Affiliations
- MRC Laboratory of Molecular Biology (GB)
- University of Dundee (GB)
- Fujirebio (Belgium) (BE)
- University of Antwerp (BE)
- Corresponding author
- Goedert, M
- Jakes, R
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1994-08-01
- Volume
- 301
- Issue
- 3
- Pages
- 871-877
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1137067
- OpenAlex
- W1958842451
- Cited by
- 375
- References
- 51
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 4 · 2023: 5 · 2022: 12 · 2021: 10 · 2020: 8 · 2019: 9 · 2018: 7 · 2017: 10 · 2016: 7 · 2015: 8 · 2014: 8 · 2013: 9 · 2012: 13
- FWCI
- 9.97
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Discovery Research Portal (University of Dundee) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- tau protein, paired helical filaments, Alzheimer's disease, epitope mapping, phosphorylation sites, neurofibrillary tangles, tau hyperphosphorylation
- MeSH terms
- Alzheimer Disease, Amino Acid Sequence, Animals, Antibodies, Monoclonal, Antibody Specificity, Epitopes, Binding Sites, Brain, Brain Chemistry, Cerebral Cortex, Humans, Molecular Sequence Data, Phosphorylation, Phosphothreonine, Recombinant Proteins, Structure-Activity Relationship, Microscopy, Immunoelectron, Neurofibrillary Tangles, tau Proteins, Rats
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