Periplasmic nitrate-reducing system of the phototrophic bacterium Rhodobacter sphaeroides DSM 158: transcriptional and mutational analysis of the napKEFDABC gene cluster
Open accessAbstract
The phototrophic bacterium Rhodobacter sphaeroidesDSM 158 is able to reduce nitrate to nitrite by means of a periplasmic nitrate reductase which is induced by nitrate and is not repressed by ammonium or oxygen. Recently, a 6.8 kb PstI DNA fragment carrying the napABCgenes coding for this periplasmic nitrate-reducing system was cloned [Reyes, Roldán, Klipp, Castillo and Moreno-Vivián (1996) Mol. Microbiol. 19, 1307–1318]. Further sequence and genetic analyses of the DNA region upstream from the napABCgenes reveal the presence of four additional napgenes. All these R. sphaeroidesgenes seem to be organized into a napKEFDABCtranscriptional unit. In addition, a partial open reading frame similar to the Azorhizobium caulinodans yntCgene and the Escherichia coli yjcCand yhjKgenes is present upstream from this napgene cluster. The R. sphaeroides napKgene codes for a putative 6.3 kDa transmembrane protein which is not similar to known proteins and the napEgene codes for a 6.7 kDa transmembrane protein similar to the Thiosphaera pantotrophaNapE. The R. sphaeroides napFgene product is a 16.4 kDa protein with four cysteine clusters that probably bind four [4Fe-4S] centres. This iron–sulphur protein shows similarity to the NapF and NapG proteins of E. coliand Haemophilus influenzae.Finally, the napDgene product is a 9.4 kDa soluble protein which is also found in E. coliand T. pantotropha. The 5´ end of the naptranscript has been determined by primer extension, and a δ70-like promoter has been identified upstream from the napKgene. The same transcriptional start site is found for cells growing aerobically or anaerobically with nitrate. Different mutant strains carrying defined polar and non-polar insertions in each napgene were constructed. Characterization of these mutant strains demonstrates the participation of the napgene products in the periplasmic nitrate reduction in R. sphaeroides.
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napB gene deletion mutants in R. sphaeroides and E. coli exhibit very little activity.
“napB gene deletion mutants in R. sphaeroides215 and E. coli102 exhibit very little activity”
From Nitrate and periplasmic nitrate reductases (2014) · cites this paper as [215]
Mutational studies on R. sphaeroides, E. coli, and Wolinella succinogenes have given insight into the significance of each auxiliary nap gene in nitrate reduction via Nap.
“Mutational studies conducted on R. sphaeroides,215 E. coli,102 and Wolinella succinogenes105 have given insight into the significance of each auxiliary nap gene in nitrate reduction via Nap.”
From Nitrate and periplasmic nitrate reductases (2014) · cites this paper as [215]
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- Authors
- REYES, F., GAVIRA, M., CASTILLO, F., & MORENO-VIVIÁN, C.
- Affiliations
- University of Córdoba (ES)
- Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Córdoba, 14071-Córdoba, Spain
- Venue
- Biochemical Journal
- Publisher
- Portland Press Ltd.
- Published
- 1998-05-01
- Volume
- 331
- Issue
- 3
- Pages
- 897-904
- ISSN
- 0264-6021 (ISSN-L)
- 1470-8728
- PMC
- PMC1219433
- OpenAlex
- W2139697583
- Cited by
- 56
- References
- 44
- Citations by year
- 2026: 1 · 2023: 3 · 2020: 1 · 2019: 1 · 2018: 5 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 1
- FWCI
- 3.25
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Biochemical Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Universidad de Sevilla
- University of East Anglia
- University of Oxford
- Universidad de Córdoba
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
- Keywords
- Rhodobacter sphaeroides, periplasmic nitrate reductase, nitrate reduction, nitrite, sigma 70 promoter, transcription start sites, Thiosphaera pantotropha
- MeSH terms
- Amino Acid Sequence, Bacterial Proteins, Cloning, Molecular, DNA Mutational Analysis, Electron Transport, Genes, Bacterial, Molecular Sequence Data, Nitrate Reductases, Paraquat, Promoter Regions, Genetic, Rhodobacter sphaeroides, Transcription, Genetic, Restriction Mapping, Gene Expression Regulation, Enzymologic, Mutagenesis, Insertional, Sequence Homology, Amino Acid, Sequence Analysis, DNA, Periplasm, Nitrate Reductase
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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