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Cited by 122T-cell and B-cell ImmunologyGut microbiota and healthGastrointestinal motility and disorders

Microbial colonization influences composition and T‐cell receptor Vβ repertoire of intraepithelial lymphocytes in rat intestine

HELGELAND, L., VAAGE, J. T., ROLSTAD, B., MIDTVEDT, T., BRANDTZAEG, P.·1996·Immunology
DOI 10.1046/j.1365-2567.1996.d01-783.x
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Abstract

Studies in mice have shown that the composition of intestinal intraepithelial lymphocytes (IEL) may be markedly altered by gut microbial colonization. Such modulation was studied in a rat model by the use of germ-free and conventionalized animals from which IEL from the small intestine were isolated and analysed by flow cytometry. Conventionalization caused expansion as well as phenotypic alterations of T-cell receptor (TCR) alpha/beta + IEL in that the proportions of CD4+ and CD8 alpha beta + TCR alpha/beta + cells were increased, while the double negative (CD4- CD8-) fraction was reduced. microbial colonization also influenced the TCR V beta repertoire of CD8+ IEL in that the proportions of V beta 8.2+ and V beta 10+ cells were increased, whereas V beta 8.5+ and V beta 16+ cells were relatively decreased. Moreover, conventionalization influenced the levels of TCR cell surface expression in the same V beta subsets. Three-colour flow-cytometric analysis demonstrated that skewing of the V beta repertoire was most pronounced in the CD8 alpha alpha + subset, although the numerical increase of IEL mainly included the CD8 alpha beta + subset. In contrast to IEL, the TCR V beta repertoire in mesenteric lymph nodes was unchanged after intestinal colonization. These results confirm that TCR alpha/beta + IEL subpopulations respond dynamically to the microbial gut flora and suggest that their V beta repertoire can be shaped by luminal microbial antigens.

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Crossref · 10.1046/j.1365-2567.1996.d01-783.x

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  • The minimalist bacterial colonization of the ASF mouse avoids the complications of high abundance and diversity in conventional mice, while overcoming host developmental hurdles seen in GF mice.

    “The minimalist bacterial colonization of the ASF mouse avoids the complications of the high abundance and diversity in conventional mice, while overcoming the host developmental hurdles seen in GF mice, such as an underdeveloped immune system (Atarashi et al., 2011;Helgeland et al., 1996), slower intestinal epithelial turnover (Savage et al., 1981), differing nutritional requirements and less body”

    From The Microbiota-Gut-Brain Axis (2019) · cites this paper as Helgeland et al., 1996

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Authors
HELGELAND, L., VAAGE, J. T., ROLSTAD, B., MIDTVEDT, T., & BRANDTZAEG, P.
Affiliations
  • University of Oslo (NO)
  • Karolinska Institutet (SE)
  • Pathologisches Institut (DE)
Corresponding author
HELGELAND, L.
ORCID
Venue
Immunology
Publisher
Wiley
Published
1996-12
Volume
89
Issue
4
Pages
494-501
ISSN
  • 0019-2805 (ISSN-L)
  • 1365-2567
DOI
10.1046/j.1365-2567.1996.d01-783.x
OpenAlex
W2040231886
Cited by
122
References
37
Citations by year
2026: 1 · 2025: 5 · 2024: 3 · 2023: 1 · 2021: 3 · 2020: 4 · 2019: 2 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 8 · 2013: 3 · 2012: 8
FWCI
1.51
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 2524 d)
Locations
  • Immunology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Norges Forskningsråd
  • Medical Research Council
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
Keywords
intraepithelial lymphocytes, gut microbiota, microbial colonization, T cell receptor V beta repertoire, germ-free rats, conventionalization, CD4+ T cells, double-negative T cells, mesenteric lymph nodes, small intestine
MeSH terms
Animals, Flow Cytometry, Germ-Free Life, Intestinal Mucosa, Lymphocyte Activation, Male, Mesentery, Rats, Inbred Strains, T-Lymphocytes, CD4-Positive T-Lymphocytes, Receptors, Antigen, T-Cell, alpha-beta, CD8-Positive T-Lymphocytes, Immunity, Mucosal, Rats

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