Spatial and temporal distribution of non-starter lactic acid bacteria in Cheddar cheese
Abstract
AIMS: The aim of this work was to investigate the spatial and temporal distribution of species and strains of non-starter lactic acid bacteria (NSLAB) within Cheddar cheese. METHODS AND RESULTS: Randomly amplified polymorphic DNA was used to identify and track the principle species and strain groups of NSLAB present. The same strains dominated each location examined within a cheese at any particular time point. Temporal change in species and strains of NSLAB during ripening was observed. A mixture of Lactobacillus paracasei, Lact. plantarum, Lact. rhamnosus and unidentified strains was found up to 6 weeks of maturation, thereafter only Lact. paracasei strains were isolated. CONCLUSION: Little variation in the spatial distribution of NSLAB strains occurs within Cheddar cheese; however, temporal changes in the species and strains were observed during ripening. SIGNIFICANCE AND IMPACT OF THE STUDY: The complex changes in the composition of the NSLAB community of Cheddar cheese may be the source of the variation in flavour that is seen in commercial practice.
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The composition of adventitious LAB varies depending on cheese type, the mode of processing, and ripening time.
“The composition of adventitious LAB varies depending on cheese type, the mode of processing, and ripening time [84,114,117,118].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [118]
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Self-citation
- Authors
- Fitzsimons, N. A., Cogan, T. M., Condon, S., & Beresford, T.
- Affiliations
- University College Cork (IE)
- ORCID
- Beresford, T.
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2001-04
- Volume
- 90
- Issue
- 4
- Pages
- 600-608
- ISSN
- 1364-5072 (ISSN-L)
- 1365-2672
- OpenAlex
- W2107389666
- Cited by
- 97
- References
- 33
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2023: 4 · 2022: 3 · 2021: 4 · 2020: 4 · 2019: 2 · 2018: 7 · 2017: 6 · 2016: 4 · 2015: 6 · 2014: 5 · 2013: 6 · 2012: 2
- FWCI
- 11.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Teagasc
- European Regional Development Fund
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
- Keywords
- non-starter lactic acid bacteria, Cheddar cheese, cheese ripening, spatial distribution, temporal distribution, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus rhamnosus, bacterial community composition, cheese flavor, RAPD-PCR
- MeSH terms
- Cheese, Food Handling, Food Microbiology, Lactobacillus, Random Amplified Polymorphic DNA Technique, Lactic Acid
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