Genetic diversity, safety and technological characterization of lactic acid bacteria isolated from artisanal Pico cheese
Abstract
A total of 114 lactic acid bacteria were isolated at one and 21 days of ripening from a traditional raw cow's milk cheese without the addition of starter culture, produced by three artisanal cheese-makers in Azores Island (Pico, Portugal). Identification to species and strain level was accomplished by16S rRNA gene and PFGE analysis. Carbohydrate utilization profiles were obtained with the relevant API kits. Isolates were evaluated according to safety and technological criteria. The most frequently observed genus identified by 16S rRNA sequencing analysis was Enterococcus, whereas API system mostly identified Lactobacillus. The highest percentages of antibiotic resistance were to nalidixic acid (95%), and aminoglycosides (64-87%). All isolates were sensitive to several beta-lactam antibiotics and negative for histamine and DNase production. Gelatinase activity was detected in 49.1% of isolates, 43% were able to degrade casein and 93% were α-hemolytic. Most enterococci presented virulence genes, such as gelE, asaI, ace. Diacetyl production was found to be species dependent and one strain (Leu. citreum) produced exopolysaccharides. Selected strains were further studied for technological application and were found to be slow acid producers in milk and experimental cheeses, a desirable trait for adjunct cultures. Two strains were selected on the basis of technological and safety application as adjunct cultures in cheese production and presented the best cheese aroma and flavor in consumer preference tests. This is the first effort to characterize Pico cheese LAB isolates for potential application as adjunct cultures; the results suggest the potential of two strains to improve the quality of this traditional raw milk product.
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Among enterococci, Enterococcus durans, E. faecalis, and E. faecium are most abundant in cheese.
“Among enterococci, Enterococcus durans, E. faecalis, and E. faecium are most abundant in cheese [102,122].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [122]
Within the genus Leuconostoc, the species Ln. mesenteroides, Ln. peseudomesenteroides, and Ln. citreum have been detected in artisanal cheeses produced from raw milk.
“Within the genus Leuconostoc, the species Ln. mesenteroides, Ln. peseudomesenteroides, and Ln. citreum have been detected in artisanal cheeses produced from raw milk [122,123,124,125].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [122]
Diacetyl is produced by some LAB strains during citrate fermentation and is present in many dairy products such as cheese.
“Diacetyl is produced by some LAB strains during citrate fermentation and is present in many dairy products such as cheese [122,166].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [122]
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- Authors
- Domingos-Lopes, M. F. P., Stanton, C., Ross, P. R., Dapkevicius, M. L. E., & Silva, C. C. G.
- Affiliations
- Teagasc - The Irish Agriculture and Food Development Authority (IE)
- Universidade dos Açores (PT)
- Corresponding author
- Silva, C.C.G.
- Venue
- Food Microbiology
- Publisher
- Elsevier BV
- Published
- 2017-05
- Volume
- 63
- Pages
- 178-190
- ISSN
- 0740-0020 (ISSN-L)
- OpenAlex
- W2555804104
- Cited by
- 210
- References
- 92
- Citations by year
- 2026: 15 · 2025: 25 · 2024: 18 · 2023: 34 · 2022: 30 · 2021: 24 · 2020: 23 · 2019: 20 · 2018: 16 · 2017: 5
- FWCI
- 17.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Food Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fundação para a Ciência e a Tecnologia (PTDC/AGR-ALI/104385/2008)
- Fundo Regional para a Ciência e Tecnologia (M3.1.2/F/009/2011)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
- Keywords
- lactic acid bacteria, artisanal cheese, Enterococcus, Lactobacillus, antibiotic resistance, virulence genes, gelatinase activity, casein degradation, adjunct cultures, cheese ripening, genetic diversity, food safety
- MeSH terms
- Animals, Anti-Bacterial Agents, Cattle, Cheese, Food Microbiology, Lactobacillaceae, Lactobacillus, Portugal, RNA, Ribosomal, 16S, Taste, Vancomycin, Genetic Variation, Virulence, Enterococcus, Drug Resistance, Multiple, Bacterial, Food Safety, Food Quality, Raw Foods
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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