Identification of plant-associated enterococci

Muller, T.; Ulrich, A.; Ott, E.-M.; Muller, M.

doi:10.1046/j.1365-2672.2001.01373.x

Cited by 184Enterobacteriaceae and Cronobacter ResearchAntibiotic Resistance in BacteriaGenomics and Phylogenetic Studies

Identification of plant-associated enterococci

Muller, T., Ulrich, A., Ott, E.-M., Muller, M.·2001·Journal of Applied Microbiology

DOI 10.1046/j.1365-2672.2001.01373.x

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Abstract

AIMS: Enterococcus isolates from forage grass were subjected to taxonomical investigations and tested for antibiotic resistance. METHODS AND RESULTS: The identification procedure included phenotypic characterizations, restriction analyses of polymerase chain reaction-amplified 16S rDNA, whole-cell protein profile analyses and 16S rDNA sequence analyses. Agar diffusion tests were performed to detect antibiotic resistance. CONCLUSION: The isolates were identified as belonging to the species Enterococcus faecium, Ent. mundtii, Ent. casseliflavus, Ent. faecalis and Ent. sulfureus. However, the majority of isolates differed distinctly in their restriction patterns from those of known species. They formed a group of a homogeneous 16S rDNA genotype (VI). The 16S rDNA sequence of a representative isolate revealed the closest relationship to the species Ent. faecalis (similarity of 97.4%). All isolates were sensitive to vancomycin, but almost all were resistant to gentamycin and streptomycin. SIGNIFICANCE AND IMPACT OF THE STUDY: The taxonomical investigations suggest that the isolates of the 16S rDNA genotype VI represent a new plant-associated Enterococcus species.

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Lactobacillus species (L. plantarum, L. brevis, L. casei, L. rhamnosus, L. curvatus, L. gasseri, L. pentosus) do not exceed 1 × 103 CFU/g.
“The LABs of the genus Lactobacillus (L. plantarum, L. brevis, L. casei, L. rhamnosus, L. curvatus, L. gasseri, L. pentosus) do not exceed 1 × 103 CFU/g.”
From Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview. (2019) · cites this paper as Müller et al. 2001
Pediococcus species presence has been observed at a number not exceeding 1 × 103 CFU/g.
“The presence of bacteria of the genus Pediococcus (P. pentosaceus, P. acidilactici, P. damnosus, P. confusa) has been observed at a number that does not exceed 1 × 103 CFU/g (Müller et al. 2001).”
From Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview. (2019) · cites this paper as Müller et al. 2001

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Authors: Muller, T., Ulrich, A., Ott, E.-M., & Muller, M.
Affiliations: Leibniz Centre for Agricultural Landscape Research (DE)
University of Rostock (DE)
ORCID: Ulrich, A.
Venue: Journal of Applied Microbiology
Publisher: Oxford University Press (OUP)
Published: 2001-08-02
Volume: 91
Issue: 2
Pages: 268-278
ISSN: 1364-5072 (ISSN-L)
1365-2672
DOI: 10.1046/j.1365-2672.2001.01373.x
OpenAlex: W2050503327
Cited by: 184
References: 52
Citations by year: 2026: 3 · 2025: 3 · 2024: 3 · 2023: 5 · 2022: 8 · 2021: 5 · 2020: 16 · 2019: 8 · 2018: 9 · 2017: 7 · 2016: 11 · 2015: 5 · 2014: 15 · 2013: 4 · 2012: 16
FWCI: 4.15
Type: journal article
Language: en
Open access: closed
Licence: doi.wiley.com/10.1002/tdm_license_1.1 (tdm, 5143 d)
Locations: Journal of Applied Microbiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Enterobacteriaceae and Cronobacter Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords: Enterococcus, forage grasses, Enterococcus faecalis, Enterococcus faecium, Enterococcus mundtii, Enterococcus casseliflavus, bacterial taxonomy, antibiotic resistance, gentamicin resistance, streptomycin resistance, vancomycin susceptibility, restriction fragment length polymorphism, whole-cell protein profiles
MeSH terms: DNA, Bacterial, Electrophoresis, Polyacrylamide Gel, Poaceae, Phylogeny, RNA, Ribosomal, 16S, Bacterial Typing Techniques, Polymerase Chain Reaction, Enterococcus, Sequence Analysis, DNA, Drug Resistance, Bacterial

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Identification of plant-associated enterococci

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