CoChat
Sign Up
Cited by 251Listeria monocytogenes in Food SafetyBacterial Genetics and BiotechnologyBiochemical and Structural Characterization

The DOI matches its Crossref record; 57 of the 57 references it cites were found in a registry or an index.

Interaction between the protein InlB of Listeria monocytogenes and lipoteichoic acid: a novel mechanism of protein association at the surface of Gram‐positive bacteria

Jonquières, Renaud, Bierne, Hélène, Fiedler, Franz, Gounon, Pierre, Cossart, Pascale·1999·Molecular Microbiology
DOI 10.1046/j.1365-2958.1999.01652.x
Open this paper in CoChat — free account

Abstract

InlB is a Listeria monocytogenes protein that is sufficient to promote entry in a variety of mammalian cells. The last 232‐amino‐acid domain (Csa) of InlB has been shown to mediate attachment on the listerial surface, although its sequence does not suggest any known mechanism of association to the bacterial surface. InlB is present both on the bacterial surface and in culture supernatants. As has been recently demonstrated, both forms of InlB, soluble and surface‐bound, can trigger signalling in host cells. To elucidate the specific role of each of the two forms, it was important to understand how InlB associates with the bacterial surface. Using microscopy, we find evidence that InlB is partially buried in the cell wall layer, and using fractionation experiments we demonstrate that InlB associates with the bacterial cytoplasmic membrane. Moreover, using purified lipoteichoic acid (LTA) and the three polypeptides InlB, Csa, or InlBΔCsa (InlB lacking the last 232 amino acids), we demonstrate that LTA is a ligand for the Csa domain of InlB. These results provide the first evidence of an interaction between lipoteichoic acids and a bacterial protein involved in adhesion and signalling, and highlight a new mechanism of protein association on the surface of Gram‐positive bacteria.

Overview

Integrity signals

Registry

Crossref · 10.1046/j.1365-2958.1999.01652.x

Cites

57 of 57 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 57 references

Research works printed but not linked: 1

  1. Harwood C.R.&Cutting S.M.et al(1990) InMolecular Biological Methods forBacillus. Harwood C.R. and Cutting S.M. (eds). John Wiley and Sons Ltd p.378.

Cited by

Sorted by citations

See all 251

Related

Metadata

Authors
Jonquières, R., Bierne, H., Fiedler, F., Gounon, P., & Cossart, P.
Affiliations
  • Institut Pasteur (FR)
  • Ludwig-Maximilians-Universität München (DE)
Corresponding author
Cossart, Pascale
ORCID
Venue
Molecular Microbiology
Publisher
Wiley
Published
1999-12
Volume
34
Issue
5
Pages
902-914
ISSN
  • 0950-382X (ISSN-L)
  • 1365-2958
DOI
10.1046/j.1365-2958.1999.01652.x
OpenAlex
W1981604960
Cited by
251
References
57
Citations by year
2026: 3 · 2025: 1 · 2024: 2 · 2023: 5 · 2022: 5 · 2021: 4 · 2020: 7 · 2019: 6 · 2018: 7 · 2017: 2 · 2016: 6 · 2015: 7 · 2014: 16 · 2013: 7 · 2012: 10
FWCI
12.68
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 821 d)
Locations
  • Molecular Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • European Commission
  • Institut National de la Recherche Agronomique
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Listeria monocytogenes in Food Safety
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
Keywords
InlB, Listeria monocytogenes, lipoteichoic acid, plasma membrane, bacterial adhesion, host cell signalling, Gram-positive bacteria
MeSH terms
Bacterial Proteins, Cell Fractionation, Cell Membrane, Cell Wall, Chromatography, Gel, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Lipopolysaccharides, Listeria monocytogenes, Membrane Proteins, Plasmids, Protoplasts, Teichoic Acids, Immunoblotting

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review