The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Localization of components of the chemotaxis machinery of Escherichia coli using fluorescent protein fusions
Open accessAbstract
We prepared fusions of yellow fluorescent protein [the YFP variant of green fluorescent protein (GFP)] with the cytoplasmic chemotaxis proteins CheY, CheZ and CheA and the flagellar motor protein FliM, and studied their localization in wild‐type and mutant cells of Escherichia coli. All but the CheA fusions were functional. The cytoplasmic proteins CheY, CheZ and CheA tended to cluster at the cell poles in a manner similar to that observed earlier for methyl‐accepting chemotaxis proteins (MCPs), but only if MCPs were present. Co‐localization of CheY and CheZ with MCPs was CheA dependent, and co‐localization of CheA with MCPs was CheW dependent, as expected. Co‐localization with MCPs was confirmed by immunofluorescence using an anti‐MCP primary antibody. The motor protein FliM appeared as discrete spots on the sides of the cell. These were seen in wild‐type cells and in a fliN mutant, but not in flhC or fliG mutants. Co‐localization with flagellar structures was confirmed by immunofluorescence using an antihook primary antibody. Surprisingly, we did not observe co‐localization of CheY with motors, even under conditions in which cells tumbled.
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Tsien, R. Y. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.509 · Cited by 6,143
Guzman, L. M., Belin, D., Carson, M. J., & Beckwith, J. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.14.4121-4130.1995 · Cited by 4,935
Polar Location of the Chemoreceptor Complex in the Escherichia coli Cell
Maddock, J. R., & Shapiro, L. (1993) · Science · doi:10.1126/science.8456299 · Cited by 708
Receptor clustering as a cellular mechanism to control sensitivity
Bray, D., Levin, M. D., & Morton-Firth, C. J. (1998) · Nature · doi:10.1038/30018 · Cited by 616
Phosphorylation of three proteins in the signaling pathway of bacterial chemotaxis
Hess, J. F., Oosawa, K., Kaplan, N., & Simon, M. I. (1988) · Cell · doi:10.1016/0092-8674(88)90489-8 · Cited by 549
Falke, J. J., Bass, R. B., Butler, S. L., Chervitz, S. A., & Danielson, M. A. (1997) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.13.1.457 · Cited by 536
Phosphorylation of bacterial response regulator proteins by low molecular weight phospho-donors.
Lukat, G. S., McCleary, W. R., Stock, A. M., & Stock, J. B. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.2.718 · Cited by 481
Structure of CheA, a Signal-Transducing Histidine Kinase
Bilwes, A. M., Alex, L. A., Crane, B. R., & Simon, M. I. (1999) · Cell · doi:10.1016/s0092-8674(00)80966-6 · Cited by 476
Four-helical-bundle structure of the cytoplasmic domain of a serine chemotaxis receptor
Kim, K. K., Yokota, H., & Kim, S.-H. (1999) · Nature · doi:10.1038/23512 · Cited by 445
Phosphorylation-dependent binding of a signal molecule to the flagellar switch of bacteria.
Welch, M., Oosawa, K., Aizawa, S., & Eisenbach, M. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.19.8787 · Cited by 423
Cited by
Bacterial chemoreceptors: high-performance signaling in networked arrays
Hazelbauer, G. L., Falke, J. J., & Parkinson, J. S. (2008) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2007.09.014 · Cited by 632
Stoichiometry and turnover in single, functioning membrane protein complexes
Leake, M. C., Chandler, J. H., Wadhams, G. H., Bai, F., Berry, R. M., & Armitage, J. P. (2006) · Nature · doi:10.1038/nature05135 · Cited by 599
Going against the grain: chemotaxis and infection in Vibrio cholerae
Butler, S. M., & Camilli, A. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1207 · Cited by 393
Greenfield, D., McEvoy, A. L., Shroff, H., Crooks, G. E., Wingreen, N. S., Betzig, E., & Liphardt, J. (2009) · PLoS Biology · doi:10.1371/journal.pbio.1000137 · Cited by 328
Jeckelmann, J.-M., & Erni, B. (2019) · Subcellular Biochemistry · doi:10.1007/978-3-030-18768-2_8 · Cited by 55
Metadata
- Authors
- Sourjik, V., & Berg, H. C.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2000-08
- Volume
- 37
- Issue
- 4
- Pages
- 740-751
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2024512146
- Cited by
- 311
- References
- 32
- FWCI
- 6.86
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Sourjik, V., & Berg, H. C. (2000). Localization of components of the chemotaxis machinery of Escherichia coli using fluorescent protein fusions. Molecular Microbiology. https://doi.org/10.1046/j.1365-2958.2000.02044.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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