The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Self-Organization of the Escherichia coli Chemotaxis Network Imaged with Super-Resolution Light Microscopy
Open accessAbstract
The Escherichia coli chemotaxis network is a model system for biological signal processing. In E. coli, transmembrane receptors responsible for signal transduction assemble into large clusters containing several thousand proteins. These sensory clusters have been observed at cell poles and future division sites. Despite extensive study, it remains unclear how chemotaxis clusters form, what controls cluster size and density, and how the cellular location of clusters is robustly maintained in growing and dividing cells. Here, we use photoactivated localization microscopy (PALM) to map the cellular locations of three proteins central to bacterial chemotaxis (the Tar receptor, CheY, and CheW) with a precision of 15 nm. We find that cluster sizes are approximately exponentially distributed, with no characteristic cluster size. One-third of Tar receptors are part of smaller lateral clusters and not of the large polar clusters. Analysis of the relative cellular locations of 1.1 million individual proteins (from 326 cells) suggests that clusters form via stochastic self-assembly. The super-resolution PALM maps of E. coli receptors support the notion that stochastic self-assembly can create and maintain approximately periodic structures in biological membranes, without direct cytoskeletal involvement or active transport.
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Cites
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
Datsenko, K. A., & Wanner, B. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.120163297 · Cited by 14,782
Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
Betzig, E., Patterson, G. H., Sougrat, R., Lindwasser, O. W., Olenych, S., Bonifacino, J. S., Davidson, M. W., Lippincott-Schwartz, J., & Hess, H. F. (2006) · Science · doi:10.1126/science.1127344 · Cited by 8,772
Self-citationConstruction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K. A., Tomita, M., Wanner, B. L., & Mori, H. (2006) · Molecular Systems Biology · doi:10.1038/msb4100050 · Cited by 7,858
THE DISTRIBUTION OF RAINDROPS WITH SIZE
Marshall, J. S., & Palmer, W. M. K. (1948) · Journal of Meteorology · doi:10.1175/1520-0469(1948)005<0165:tdorws>2.0.co;2 · Cited by 3,670
Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy
Hess, S. T., Girirajan, T. P. K., & Mason, M. D. (2006) · Biophysical Journal · doi:10.1529/biophysj.106.091116 · Cited by 3,605
Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy
Huang, B., Wang, W., Bates, M., & Zhuang, X. (2008) · Science · doi:10.1126/science.1153529 · Cited by 2,796
Precise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Gustafsson, M. G. L. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0406877102 · Cited by 2,224
Cherepanov, P. P., & Wackernagel, W. (1995) · Gene · doi:10.1016/0378-1119(95)00193-a · Cited by 1,937
Berg, H. C., & Purcell, E. M. (1977) · Biophysical Journal · doi:10.1016/s0006-3495(77)85544-6 · Cited by 1,758
Cited by
Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells
Huang, B., Babcock, H., & Zhuang, X. (2010) · Cell · doi:10.1016/j.cell.2010.12.002 · Cited by 1,156
Superresolution Imaging using Single-Molecule Localization
Patterson, G., Davidson, M., Manley, S., & Lippincott-Schwartz, J. (2010) · Annual Review of Physical Chemistry · doi:10.1146/annurev.physchem.012809.103444 · Cited by 527
Rapid, accurate particle tracking by calculation of radial symmetry centers
Parthasarathy, R. (2012) · Nature Methods · doi:10.1038/nmeth.2071 · Cited by 447
Wang, S., Moffitt, J. R., Dempsey, G. T., Xie, X. S., & Zhuang, X. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1406593111 · Cited by 337
Gudheti, M. V., Curthoys, N. M., Gould, T. J., Kim, D., Gunewardene, M. S., Gabor, K. A., Gosse, J. A., Kim, C. H., Zimmerberg, J., & Hess, S. T. (2013) · Biophysical Journal · doi:10.1016/j.bpj.2013.03.054 · Cited by 106
Metadata
- Authors
- Greenfield, D., McEvoy, A. L., Shroff, H., Crooks, G. E., Wingreen, N. S., Betzig, E., & Liphardt, J.
- Venue
- PLoS Biology
- Publisher
- Public Library of Science (PLoS)
- Published
- 2009-06-23
- Volume
- 7
- Issue
- 6
- Pages
- e1000137
- ISSN
- 1544-9173 (ISSN-L)
- 1545-7885
- OpenAlex
- W3023958910
- Cited by
- 328
- References
- 45
- FWCI
- 20.14
- Type
- journal article
- Language
- en
- Open access
- gold
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- Greenfield, D., McEvoy, A. L., Shroff, H., Crooks, G. E., Wingreen, N. S., Betzig, E., & Liphardt, J. (2009). Self-Organization of the Escherichia coli Chemotaxis Network Imaged with Super-Resolution Light Microscopy. PLoS Biology. https://doi.org/10.1371/journal.pbio.1000137
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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