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A genome‐wide strategy for the identification of essential genes in Staphylococcus aureus
Open accessAbstract
Summary: To address the need for new approaches to antibiotic drug development, we have identified a large number of essential genes for the bacterial pathogen, Staphylococcus aureus, using a rapid shotgun antisense RNA method. Staphylococcus aureus chromosomal DNA fragments were cloned into a xylose‐inducible expression plasmid and transformed into S. aureus. Homology comparisons between 658 S. aureus genes identified in this particular antisense screen and the Mycoplasma genitalium genome, which contains 517 genes in total, yielded 168 conserved genes, many of which appear to be essential in M. genitalium and other bacteria. Examples are presented in which expression of an antisense RNA specifically reduces its cognate mRNA. A cell‐based, drug‐screening assay is also described, wherein expression of an antisense RNA confers specific sensitivity to compounds targeting that gene product. This approach enables facile assay development for high throughput screening for any essential gene, independent of its biochemical function, thereby greatly facilitating the search for new antibiotics.
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Cited by
Essential genes of a minimal bacterium
Glass, J. I., Assad-Garcia, N., Alperovich, N., Yooseph, S., Lewis, M. R., Maruf, M., Hutchison, C. A., Smith, H. O., & Venter, J. C. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0510013103 · Cited by 912
Determination of the Core of a Minimal Bacterial Gene Set
Gil, R., Silva, F. J., Peretó, J., & Moya, A. (2004) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.68.3.518-537.2004 · Cited by 573
Actinomycetes: Still a source of novel antibiotics
Genilloud, O. (2017) · Natural Product Reports · doi:10.1039/c7np00026j · Cited by 465
Staphylococcus aureus Shifts toward Commensalism in Response to Corynebacterium Species
Ramsey, M. M., Freire, M. O., Gabrilska, R. A., Rumbaugh, K. P., & Lemon, K. P. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.01230 · Cited by 299
Gene essentiality determines chromosome organisation in bacteria
Rocha, E. P. C. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg859 · Cited by 166
Metadata
- Authors
- Forsyth, R. A., Haselbeck, R. J., Ohlsen, K. L., Yamamoto, R. T., Xu, H., Trawick, J. D., Wall, D., Wang, L., Brown‐Driver, V., Froelich, J. M., C., K. G., King, P., McCarthy, M., Malone, C., Misiner, B., Robbins, D., Tan, Z., Zhu, Z., Carr, G., Mosca, D. A., Zamudio, C., Foulkes, J. G., & Zyskind, J. W.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2002-03
- Volume
- 43
- Issue
- 6
- Pages
- 1387-1400
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2152238416
- Cited by
- 487
- References
- 0
- FWCI
- 10.51
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Forsyth, R. A., Haselbeck, R. J., Ohlsen, K. L., Yamamoto, R. T., Xu, H., Trawick, J. D., Wall, D., Wang, L., Brown‐Driver, V., Froelich, J. M., C., K. G., King, P., McCarthy, M., Malone, C., Misiner, B., Robbins, D., Tan, Z., Zhu, Z., Carr, G., . . . Zyskind, J. W. (2002). A genome‐wide strategy for the identification of essential genes in Staphylococcus aureus. Molecular Microbiology. https://doi.org/10.1046/j.1365-2958.2002.02832.x
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