The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
CsrA regulates glycogen biosynthesis by preventing translation of glgC in Escherichia coli
Open accessAbstract
Summary: The carbon storage regulatory system of Escherichia coli controls the expression of genes involved in carbohydrate metabolism and cell motility. CsrA binding to glgCAP transcripts inhibits glycogen metabolism by promoting glgCAP mRNA decay. CsrB RNA functions as an antagonist of CsrA by sequestering this protein and preventing its action. In this paper, we elucidate further the mechanism of CsrA‐mediated glgC regulation. Results from gel shift assays demonstrate that several molecules of CsrA can bind to each glgC transcript. RNA footprinting studies indicate that CsrA binds to the glgCAP leader transcript at two positions. One of these sites overlaps the glgC Shine–Dalgarno sequence, whereas the other CsrA target is located further upstream in an RNA hairpin. Results from toeprint and cell‐free translation experiments indicate that bound CsrA prevents ribosome binding to the glgC Shine–Dalgarno sequence and that this reduces GlgC synthesis. The effect of two deletions in the upstream binding site was examined. Both of these deletions reduced, but did not eliminate, CsrA binding in vitro and CsrA‐dependent regulation in vivo. Our findings establish that bound CsrA inhibits initiation of glgC translation, thereby reducing glycogen biosynthesis. This inhibition of translation probably contributes to destabilization of the glgC transcript that was observed previously.
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Cites
Experiments in molecular genetics
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Global regulation by the small RNA‐binding protein CsrA and the non‐coding RNA molecule CsrB
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Self-citationLiu, M. Y., Gui, G., Wei, B., Preston, J. F., Oakford, L., Yüksel, Ü., Giedroc, D. P., & Romeo, T. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.28.17502 · Cited by 439
Self-citationBiofilm Formation and Dispersal under the Influence of the Global Regulator CsrA of Escherichia coli
Jackson, D. W., Suzuki, K., Oakford, L., Simecka, J. W., Hart, M. E., & Romeo, T. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.1.290-301.2002 · Cited by 425
Self-citation[27] Extension inhibition analysis of translation initiation complexes
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A novel sRNA component of the carbon storage regulatory system of Escherichia coli
Weilbacher, T., Suzuki, K., Dubey, A. K., Wang, X., Gudapaty, S., Morozov, I., Baker, C. S., Georgellis, D., Babitzke, P., & Romeo, T. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03459.x · Cited by 379
Self-citationDetermination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA
Brencic, A., & Lory, S. (2009) · Molecular Microbiology · doi:10.1111/j.1365-2958.2009.06670.x · Cited by 346
Regulation of Bacterial Virulence by Csr (Rsm) Systems
Vakulskas, C. A., Potts, A. H., Babitzke, P., Ahmer, B. M. M., & Romeo, T. (2015) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00052-14 · Cited by 346
Self-citationRegulatory Circuitry of the CsrA/CsrB and BarA/UvrY Systems of Escherichia coli
Suzuki, K., Wang, X., Weilbacher, T., Pernestig, A.-K., Melefors, O., Georgellis, D., Babitzke, P., & Romeo, T. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.18.5130-5140.2002 · Cited by 286
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Metadata
- Authors
- Baker, C. S., Morozov, I., Suzuki, K., Romeo, T., & Babitzke, P.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2002-06
- Volume
- 44
- Issue
- 6
- Pages
- 1599-1610
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W1895859646
- Cited by
- 280
- References
- 28
- FWCI
- 5.67
- Type
- journal article
- Language
- en
- Open access
- bronze
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