The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.
Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA
Open accessAbstract
Summary: One of the prokaryotic post‐transcriptional regulatory mechanisms involves the CsrA/RsmA family of proteins that act by modulating translation initiation at target mRNAs. In this study, we identified the regulon of RsmA of the Pseudomonas aeruginosa PAK strain by using cultures in the stationary phase of growth. The RsmA regulon includes over 500 genes, of which approximately one‐third were affected by an rsmA mutation negatively, while the rest were affected positively. By isolating RsmA/mRNA complexes, analysing transcriptional and translational fusions, and performing gel‐shift analyses, we identified 40 genes in six operons that are regulated by RsmA directly at the level of translation. All of these genes were affected by RsmA negatively and include genes encoding the type VI secretion system HSI‐I, which has been implicated in the P. aeruginosa chronic infections. On the other hand, we were unable to demonstrate a direct interaction of RsmA with transcripts that are positively affected by this protein, including mRNAs encoding the type III secretion system and the type IV pili genes. Our work supports a model in which RsmA acts as a negative translational regulator, and where its positive effects are achieved indirectly by RsmA‐mediated interference with translation of specific regulatory factors.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular cloning: A laboratory manual
Deininger, P. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,682
Mfold web server for nucleic acid folding and hybridization prediction
Zuker, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg595 · Cited by 13,404
Bacterial Biofilms: An Emerging Link to Disease Pathogenesis
Parsek, M. R., & Singh, P. K. (2003) · Annual Review of Microbiology · doi:10.1146/annurev.micro.57.030502.090720 · Cited by 1,562
Quorum-sensing signals indicate that cystic fibrosis lungs are infected with bacterial biofilms
Singh, P. K., Schaefer, A. L., Parsek, M. R., Moninger, T. O., Welsh, M. J., & Greenberg, E. P. (2000) · Nature · doi:10.1038/35037627 · Cited by 1,519
Establishment of infection: lessons from a versatile opportunist
Lyczak, J. B., Cannon, C. L., & Pier, G. B. (2000) · Microbes and Infection · doi:10.1016/s1286-4579(00)01259-4 · Cited by 1,341
Genetic adaptation by Pseudomonas aeruginosa to the airways of cystic fibrosis patients
Smith, E. E., Buckley, D. G., Wu, Z., Saenphimmachak, C., Hoffman, L. R., D’Argenio, D. A., Miller, S. I., Ramsey, B. W., Speert, D. P., Moskowitz, S. M., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0602138103 · Cited by 1,319
A Virulence Locus of Pseudomonas aeruginosa Encodes a Protein Secretion Apparatus
Mougous, J. D., Cuff, M. E., Raunser, S., Shen, A., Zhou, M., Gifford, C. A., Goodman, A. L., Joachimiak, G., Ordoñez, C. L., Lory, S., et al. (2006) · Science · doi:10.1126/science.1128393 · Cited by 1,103
Self-citationBiofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants
Klausen, M., Heydorn, A., Ragas, P., Lambertsen, L., Aaes‐Jørgensen, A., Molin, S., & Tolker‐Nielsen, T. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03525.x · Cited by 1,020
Goodman, A. L., Kulasekara, B., Rietsch, A., Boyd, D., Smith, R. S., & Lory, S. (2004) · Developmental Cell · doi:10.1016/j.devcel.2004.08.020 · Cited by 628
Self-citationSiderophore-mediated signaling regulates virulence factor production in Pseudomonas aeruginosa
Lamont, I. L., Beare, P. A., Ochsner, U., Vasil, A. I., & Vasil, M. L. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.092016999 · Cited by 584
Cited by
Cell-surface signaling in Pseudomonas: stress responses, iron transport, and pathogenicity
Llamas, M. A., Imperi, F., Visca, P., & Lamont, I. L. (2014) · FEMS Microbiology Reviews · doi:10.1111/1574-6976.12078 · Cited by 176
The role of RNA regulators, quorum sensing and c‐di‐GMP in bacterial biofilm formation
Condinho, M., Carvalho, B., Cruz, A., Pinto, S. N., Arraiano, C. M., & Pobre, V. (2023) · FEBS Open Bio · doi:10.1002/2211-5463.13389 · Cited by 64
Gerovac, M., Wicke, L., Chihara, K., Schneider, C., Lavigne, R., & Vogel, J. (2021) · mBio · doi:10.1128/mbio.03454-20 · Cited by 40
Metadata
- Authors
- Brencic, A., & Lory, S.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2009-04-21
- Volume
- 72
- Issue
- 3
- Pages
- 612-632
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2120697993
- Cited by
- 346
- References
- 61
- FWCI
- 14.44
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA. https://app.cochat.ai/papers/10.1111/j.1365-2958.2009.06670.x
- The paper
- Brencic, A., & Lory, S. (2009). Determination of the regulon and identification of novel mRNA targets of Pseudomonas aeruginosa RsmA. Molecular Microbiology. https://doi.org/10.1111/j.1365-2958.2009.06670.x
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
