The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants
Open accessAbstract
Summary: Biofilm formation by Gfp‐tagged Pseudomonas aeruginosa PAO1 wild type, flagella and type IV pili mutants in flow chambers irrigated with citrate minimal medium was characterized by the use of confocal laser scanning microscopy and comstat image analysis. Flagella and type IV pili were not necessary for P. aeruginosa initial attachment or biofilm formation, but the cell appendages had roles in biofilm development, as wild type, flagella and type IV pili mutants formed biofilms with different structures. Dynamics and selection during biofilm formation were investigated by tagging the wild type and flagella/type IV mutants with Yfp and Cfp and performing time‐lapse confocal laser scanning microscopy in mixed colour biofilms. The initial microcolony formation occurred by clonal growth, after which wild‐type P. aeruginosa bacteria spread over the substratum by means of twitching motility. The wild‐type biofilms were dynamic compositions with extensive motility, competition and selection occurring during development. Bacterial migration prevented the formation of larger microcolonial structures in the wild‐type biofilms. The results are discussed in relation to the current model for P. aeruginosa biofilm development.
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Cites
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Biofilm formation as microbial development
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The Involvement of Cell-to-Cell Signals in the Development of a Bacterial Biofilm
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Biofilms as Complex Differentiated Communities
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Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development
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[1] Production of single-stranded plasmid DNA
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Extracellular DNA Required for Bacterial Biofilm Formation
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Self-citationQuantification of biofilm structures by the novel computer program comstat
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The role of bacterial biofilms in chronic infections
Bjarnsholt, T. (2013) · APMIS · doi:10.1111/apm.12099 · Cited by 1,080
The clinical impact of bacterial biofilms
Høiby, N., Ciofu, O., Johansen, H. K., Song, Z., Moser, C., Jensen, P. Ø., Molin, S., Givskov, M., Tolker‐Nielsen, T., & Bjarnsholt, T. (2011) · International Journal of Oral Science · doi:10.4248/ijos11026 · Cited by 894
Self-citationBjarnsholt, T., Alhede, M., Alhede, M., Eickhardt-Sørensen, S. R., Moser, C., Kühl, M., Jensen, P. Ø., & Høiby, N. (2013) · Trends in Microbiology · doi:10.1016/j.tim.2013.06.002 · Cited by 764
The developmental model of microbial biofilms: ten years of a paradigm up for review
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Roles of Cyclic Diguanylate in the Regulation of Bacterial Pathogenesis
Tamayo, R., Pratt, J. T., & Camilli, A. (2007) · Annual Review of Microbiology · doi:10.1146/annurev.micro.61.080706.093426 · Cited by 486
Metadata
- Authors
- Klausen, M., Heydorn, A., Ragas, P., Lambertsen, L., Aaes‐Jørgensen, A., Molin, S., & Tolker‐Nielsen, T.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2003-05-19
- Volume
- 48
- Issue
- 6
- Pages
- 1511-1524
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2150927592
- Cited by
- 1,020
- References
- 49
- FWCI
- 12.55
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Klausen, M., Heydorn, A., Ragas, P., Lambertsen, L., Aaes‐Jørgensen, A., Molin, S., & Tolker‐Nielsen, T. (2003). Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants. Molecular Microbiology. https://doi.org/10.1046/j.1365-2958.2003.03525.x
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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