The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Bile salts and fatty acids induce the expression of Escherichia coli AcrAB multidrug efflux pump through their interaction with Rob regulatory protein
Open accessAbstract
Summary: AcrAB of Escherichia coli, an archetype among bacterial multidrug efflux pumps, exports an extremely wide range of substrates including solvents, dyes, detergents and antimicrobial agents. Its expression is regulated by three XylS/AraC family regulators, MarA, SoxS and Rob. Although MarA and SoxS regulation works by the alteration of their own expression levels, it was not known how Rob, which is constitutively expressed, exerts its regulatory action. We show here that the induction of the AcrAB efflux pump by decanoate and the more lipophilic unconjugated bile salts is mediated by Rob, and that the low‐molecular‐weight inducers specifically bind to the C‐terminal, non‐DNA‐binding domain of Rob. Induction of Rob is not needed for induction of AcrAB, and we suggest that the inducers act by producing conformational alterations in pre‐existing Rob, as was suggested recently (Rosner, Dangi, Gronenborn and Martin, J Bacteriol 184: 1407–1416, 2002). Decanoate and unconjugated bile salts, which are present in the normal habitat of E. coli, were further shown to make the bacteria more resistant to lipophilic antibiotics, at least in part because of the induction of the AcrAB efflux pump. Thus, it is likely that E. coli is protecting itself by the Rob‐mediated upregulation of AcrAB against the harmful effects of bile salts and fatty acids in the intestinal tract.
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Cites
Multidrug efflux pumps of gram-negative bacteria
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Self-citationArac/XylS family of transcriptional regulators.
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Genes acrA and acrB encode a stress‐induced efflux system of Escherichia coli
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Molecular cloning and characterization of acrA and acrE genes of Escherichia coli
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Cited by
Molecular Basis of Bacterial Outer Membrane Permeability Revisited
Nikaido, H. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.4.593-656.2003 · Cited by 4,316
Self-citationThe Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria
Li, X.-Z., Plésiat, P., & Nikaido, H. (2015) · Clinical Microbiology Reviews · doi:10.1128/cmr.00117-14 · Cited by 1,614
Self-citationClinically Relevant Chromosomally Encoded Multidrug Resistance Efflux Pumps in Bacteria
Piddock, L. J. V. (2006) · Clinical Microbiology Reviews · doi:10.1128/cmr.19.2.382-402.2006 · Cited by 1,231
Efflux-mediated antimicrobial resistance
Poole, K. (2005) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dki171 · Cited by 1,082
Bacterial multidrug efflux pumps: Mechanisms, physiology and pharmacological exploitations
Sun, J., Deng, Z., & Yan, A. (2014) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2014.05.090 · Cited by 746
Metadata
- Authors
- Rosenberg, E. Y., Bertenthal, D., Nilles, M. L., Bertrand, K. P., & Nikaido, H.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2003-05-15
- Volume
- 48
- Issue
- 6
- Pages
- 1609-1619
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W1579756873
- Cited by
- 468
- References
- 37
- FWCI
- 11.44
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Rosenberg, E. Y., Bertenthal, D., Nilles, M. L., Bertrand, K. P., & Nikaido, H. (2003). Bile salts and fatty acids induce the expression of Escherichia coli AcrAB multidrug efflux pump through their interaction with Rob regulatory protein. Molecular Microbiology. https://doi.org/10.1046/j.1365-2958.2003.03531.x
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