Chromosomal constraints in Gram‐positive bacteria revealed by artificial inversions
Abstract
Summary: We used artificial chromosome inversions to investigate the chromosomal constraints that preserve genome organization in the Gram‐positive bacterium Lactococcus lactis. Large inversions, 80–1260 kb in length, disturbing the symmetry of the origin and terminus of the replication axis to various extents, were constructed using the site‐specific Cre‐loxP recombination system. These inversions were all mechanistically feasible and fell into various classes according to stability and effect on cell fitness. The L. lactis chromosome supports only to some extent unbalance in length of its replication arms. The location of detrimental inversions allowed identification of two constrained chromosomal regions: a large domain covering one fifth of the genome that encompasses the origin of replication (Ori domain), and a smaller domain located at the opposite of the chromosome (Ter domain).
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Previous explanations for possible mechanisms underlying chromosomal inversion included illegitimate recombination, intra-chromosomally homologous recombination, and integrase-mediated site-specific recombination.
“Based on these molecular evidences, previous explanations for the possible mechanisms underlying chromosomal inversion included illegitimate recombination,51–53 intra-chromosomally homologous recombination,54 as well as integrase-mediated site-specific recombination.55 , 56”
From Whole genomic DNA sequencing and comparative genomic analysis of<i>Arthrospira platensis</i>: high genome plasticity and genetic diversity (2016) · cites this paper as [55]
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- Authors
- Campo, N., Dias, M. J., Daveran‐Mingot, M. ‐., Ritzenthaler, P., & Le Bourgeois, P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Ritzenthaler, P.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2004-01
- Volume
- 51
- Issue
- 2
- Pages
- 511-522
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W2102183510
- Cited by
- 68
- References
- 69
- Citations by year
- 2026: 3 · 2024: 5 · 2023: 4 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 4 · 2018: 2 · 2017: 1 · 2016: 5 · 2015: 3 · 2014: 5 · 2013: 3 · 2012: 3
- FWCI
- 2.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministère de l'Enseignement Supérieur et de la Recherche
- Centre National de la Recherche Scientifique
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genome Rearrangement Algorithms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- Lactococcus lactis, Gram-positive bacteria, genome organization, origin of replication, Cre-loxP recombination, inversion stability, cell fitness
- MeSH terms
- Chromosomes, Bacterial, DNA, Bacterial, Gram-Positive Bacteria, Chromosome Inversion, Models, Genetic, Plasmids, Recombination, Genetic, Restriction Mapping, Electrophoresis, Gel, Pulsed-Field
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