Cited by 68Bacterial Genetics and BiotechnologyGenome Rearrangement AlgorithmsChromosomal and Genetic Variations

Chromosomal constraints in Gram‐positive bacteria revealed by artificial inversions

Campo, N., Dias, M. J., Daveran‐Mingot, M. ‐L., Ritzenthaler, P., Le Bourgeois, P.·2004·Molecular Microbiology

Abstract

Summary: We used artificial chromosome inversions to investigate the chromosomal constraints that preserve genome organization in the Gram‐positive bacterium Lactococcus lactis. Large inversions, 80–1260 kb in length, disturbing the symmetry of the origin and terminus of the replication axis to various extents, were constructed using the site‐specific Cre‐loxP recombination system. These inversions were all mechanistically feasible and fell into various classes according to stability and effect on cell fitness. The L. lactis chromosome supports only to some extent unbalance in length of its replication arms. The location of detrimental inversions allowed identification of two constrained chromosomal regions: a large domain covering one fifth of the genome that encompasses the origin of replication (Ori domain), and a smaller domain located at the opposite of the chromosome (Ter domain).

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Crossref · 10.1046/j.1365-2958.2003.03847.x

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  • Previous explanations for possible mechanisms underlying chromosomal inversion included illegitimate recombination, intra-chromosomally homologous recombination, and integrase-mediated site-specific recombination.

    “Based on these molecular evidences, previous explanations for the possible mechanisms underlying chromosomal inversion included illegitimate recombination,51–53 intra-chromosomally homologous recombination,54 as well as integrase-mediated site-specific recombination.55 , 56”

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Authors
Campo, N., Dias, M. J., Daveran‐Mingot, M. ‐., Ritzenthaler, P., & Le Bourgeois, P.
Affiliations
Centre National de la Recherche Scientifique (FR)
Corresponding author
Ritzenthaler, P.
Venue
Molecular Microbiology
Publisher
Wiley
Published
2004-01
Volume
51
Issue
2
Pages
511-522
ISSN
  • 0950-382X (ISSN-L)
  • 1365-2958
OpenAlex
W2102183510
Cited by
68
References
69
Citations by year
2026: 3 · 2024: 5 · 2023: 4 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 4 · 2018: 2 · 2017: 1 · 2016: 5 · 2015: 3 · 2014: 5 · 2013: 3 · 2012: 3
FWCI
2.85
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministère de l'Enseignement Supérieur et de la Recherche
  • Centre National de la Recherche Scientifique
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genome Rearrangement Algorithms
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
Keywords
Lactococcus lactis, Gram-positive bacteria, genome organization, origin of replication, Cre-loxP recombination, inversion stability, cell fitness
MeSH terms
Chromosomes, Bacterial, DNA, Bacterial, Gram-Positive Bacteria, Chromosome Inversion, Models, Genetic, Plasmids, Recombination, Genetic, Restriction Mapping, Electrophoresis, Gel, Pulsed-Field

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