Detection and possible role of two large nondivisible zones on the Escherichia coli chromosome.
Open accessAbstract
Inversion of many predetermined segments of the Escherichia coli chromosome was attempted by using a system for in vivo selection of genomic rearrangements. Two types of constraints on these inversions were observed: (i) a sensitivity to rich medium when the distance between oriC and the 86- to 91-min region (which carries loci essential for transcription and translation) is increased; (ii) a poor viability or inviability of inversions having at least one endpoint in the one-third of the chromosome around replication terminators (with an exception for some inversions ending between these terminators). Although the first constraint is simply explained by a decreased dosage of the region involved, the second one may result from disruption of two long-range chromosomal organizations. The nondivisible zones thus disclosed coincide remarkably well with the two zones that we have previously described, which are polarized with respect to their replication. It is proposed that the two phenomena result from a sequence-dependent and polarized organization of the terminal region of the chromosome, which defines chromosome replication arms and may participate in nucleoid organization.
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Previous explanations for possible mechanisms underlying chromosomal inversion included illegitimate recombination, intra-chromosomally homologous recombination, and integrase-mediated site-specific recombination.
“Based on these molecular evidences, previous explanations for the possible mechanisms underlying chromosomal inversion included illegitimate recombination,51–53 intra-chromosomally homologous recombination,54 as well as integrase-mediated site-specific recombination.55 , 56”
From Whole genomic DNA sequencing and comparative genomic analysis of<i>Arthrospira platensis</i>: high genome plasticity and genetic diversity (2016) · cites this paper as [54]
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- Authors
- Rebollo, J. E., François, V, & Louarn, J. M.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1988-12
- Volume
- 85
- Issue
- 24
- Pages
- 9391-9395
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC282758
- OpenAlex
- W2041856639
- Cited by
- 90
- References
- 11
- Citations by year
- 2026: 1 · 2024: 1 · 2022: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 2 · 2014: 5 · 2013: 3 · 2012: 6
- FWCI
- 2.66
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- Escherichia coli chromosome, chromosomal inversions, chromosome replication, nucleoid organization, dosage sensitivity, chromosome organization
- MeSH terms
- Chromosome Mapping, Chromosomes, Escherichia coli, Chromosome Inversion, Phenotype
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