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Cited by 66Multiple Sclerosis Research StudiesNeurogenesis and neuroplasticity mechanismsNerve injury and regeneration

The expression of myelin protein mRNAs during remyelination of lysolecithin‐induced demyelination

Woodruff, Franklin·1999·Neuropathology and Applied Neurobiology
DOI 10.1046/j.1365-2990.1999.00172.x
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Abstract

To gain insights into the mechanisms of myelin repair in the CNS and to establish the extent to which this process resembles myelination in development we have examined the patterns of expression of transcripts of the major myelin proteins, myelin basic protein (MBP) and proteolipid protein (PLP) during remyelination of lysolecithin‐induced demyelination in the adult rat spinal cord. Injection of 1 μl 1% lysolecithin into the dorsal funiculus caused a dramatic decrease in levels of MBP exon 1 and MBP exon 2‐containing transcripts and PLP/DM20 transcripts. Between 10 and 21 days post‐lesion induction there was a gradual increase in levels of expression of all transcripts, which had returned to levels associated with normally myelinated spinal cord white matter at 21 days. These increases in levels of expression corresponded to the appearance of remyelinated axons, detected on toluidine blue‐stained resin sections. Foci of high levels of expression occurred in regions of the lesion in which new myelin sheath formation was occurring, although the level of expression throughout the lesion never exceeded levels associated with myelin sheath maintenance in normal white matter due to the asynchronous pattern of remyelination. The changes in levels of expression of MBP exon 2 closely followed those of MBP exon 1. Our results indicate that (i) myelin protein gene expression associated with myelinogenesis during remyelination follows a similar pattern to that of myelinogenesis during development and that (ii) in rat models of demyelination changes of expression of MBP exon 1 and exon 2‐containing transcripts are of equal value, an observation relevant to quantifying the effects of putative remyelination‐enhancing strategies using the lysolecithin model.

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  • The same switch from an M1 to M2 response during remyelination was observed in another model of demyelination induced by injection of ethidium bromide into the rat caudal cerebellar peduncles (CCP).

    “We also observed the same switch from an M1 to M2 response during remyelination in another model of demyelination induced by injection of ethidium bromide into the rat caudal cerebellar peduncles (CCP) (Supplementary Fig. 2 online)14.”

    From M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination (2013) · cites this paper as [14]

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Authors
Woodruff, & Franklin
Affiliations
University of Cambridge (GB)
Corresponding author
Franklin
Venue
Neuropathology and Applied Neurobiology
Publisher
Wiley
Published
1999-06
Volume
25
Issue
3
Pages
226-235
ISSN
  • 0305-1846 (ISSN-L)
  • 1365-2990
DOI
10.1046/j.1365-2990.1999.00172.x
OpenAlex
W2083239573
Cited by
66
References
39
Citations by year
2026: 1 · 2024: 3 · 2021: 2 · 2020: 1 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 1 · 2012: 3
FWCI
2.33
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 948 d)
Locations
  • Neuropathology and Applied Neurobiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Wellcome Trust
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
Keywords
remyelination, demyelination, lysolecithin-induced demyelination, myelin basic protein, proteolipid protein, spinal cord, myelination, demyelinating disease model
MeSH terms
Animals, Autoradiography, Demyelinating Diseases, Myelin Basic Protein, Exons, Female, Lysophosphatidylcholines, Myelin Proteins, Myelin Sheath, Rats, Inbred Strains, RNA, Messenger, Spinal Cord, Oligonucleotide Probes, In Situ Hybridization, Myelin Proteolipid Protein, Rats

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