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Utility of antibodies against a 22 kD molecule of Dirofilaria immitis in the diagnosis of human pulmonary dirofilariasis
Open accessAbstract
To assess the characteristics of an ELISA test for the diagnosis of human pulmonary dirofilariasis, we studied the sera of 24 subjects with other helmintoses and of 37 patients suffering from non-parasitic focal lung diseases, comparing them with negative and positive sera. ELISA and Western blot with complete somatic antigen and ELISA with protein Di22 (specifically recognized in cases of lung dirofilariasis) were performed. With ELISA SA the false positive rate was 25% in cases with other parasitoses and 30% in cases with focal lung diseases. ELISA Di22 decreases this positivity levels. Only 2 cases with visceral larva migrans (8.3%) and a case with lung nodules metastatic from renal adenocarcinoma (2.7%) were positive. ELISA Di22 therefore greatly decreases the false positive rate of ELISA SA.
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“Seroepidemiological studies of residents of areas of endemicity reported higher rates of infection in a similar manner to those of canines of the same areas (1, 5, 13, 16, 17, 34).”
From Seroepidemiological Study of Canine and Human Dirofilariasis in the Endemic Region of Northern Serbia (2020) · cites this paper as [34]
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Cites
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“Group B (positive controls) consisted of three patients with human pulmonary dirofilariosis detected by a previously reported case finding study (Cordero et al. 1990, 1992a).”
Background
Research works printed but not linked: 2
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“In ELISAs, this protein exhibits 100% sensitivity and 90% specificity, with positive and negative predictive values of 75% and 100%, respectively (336).”
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Human Dirofilariasis in the European Union
Muro, A., Genchi, C., Cordero, M., & Simón, F. (1999) · Parasitology Today · doi:10.1016/s0169-4758(99)01496-9 · Cited by 137
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Self-citationHelminth-related Eosinophilia in African Immigrants, Gran Canaria
Pardo, J., Carranza‐Rodriguez, C., Muro, A., Ángel-Moreno, A., Martin, A.-M., Martin, T., Hernández-Cabrera, M., & Pérez-Arellano, J.-L. (2006) · Emerging infectious diseases · doi:10.3201/eid1210.060102 · Cited by 86
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Walther, M. R., & Muller, R. (2003) · Advances in Parasitology/Advances in parasitology · doi:10.1016/s0065-308x(03)53004-4 · Cited by 56
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“These results were additionally confirmed using western blot analysis performed according to a previously described methodology (23, 24).”
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“No entanto, é necessária uma análise de um número maior de soros para validar esse teste(12).”
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“Seroepidemiological studies of residents of areas of endemicity reported higher rates of infection in a similar manner to those of canines of the same areas (1, 5, 13, 16, 17, 34).”
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“A 22-kDa (Di22) molecule from the adult somatic antigenic complex (SA), allows the identification of human pulmonary cases (Perera et al. 1994; Perera et al. (1998).”
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“Recombinant proteins proved to exhibit 100% sensitivity and 90% specificity by ELISA for the diagnosis of pulmonary dirofilariasis [176].”
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“evaluated the utility of anti-Di22 antibodies in the diagnosis of human pulmonary dirofilariasis using sera obtained from patients (Perera et al., 1998).”
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“…immunosorbent assays (ELISAs) developed by our research group, which use antigens from adult whole worms of the species Dirofilaria immitis [18] and Schistosoma bovis [19,20], excretion/secretion antigens of Fasciola hepatica [21], and somatic larvae antigens of Strongyloides venezuelensis…”
Metadata
- Authors
- Perera, L., Pérez‐Arellano, J. L., Cordero, M., Simón, F., & Muro, A.
- Affiliations
- Universidad de Salamanca (ES)
- Corresponding author
- Muro, A.
- Venue
- Tropical Medicine & International Health
- Publisher
- Wiley
- Published
- 1998-02
- Volume
- 3
- Issue
- 2
- Pages
- 151-155
- ISSN
- 1360-2276 (ISSN-L)
- 1365-3156
- OpenAlex
- W2051191410
- Cited by
- 33
- References
- 13
- Citations by year
- 2026: 1 · 2020: 4 · 2018: 2 · 2017: 2 · 2016: 4 · 2014: 2 · 2012: 2
- FWCI
- 1.01
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Tropical Medicine & International Health (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
- Life Sciences › Immunology and Microbiology › Parasitology › Parasitic infections in humans and animals
- Keywords
- Dirofilaria immitis, ELISA, serological diagnosis, false positive rate, visceral larva migrans, Western blot
- MeSH terms
- Aged, Animals, Antibodies, Helminth, Dirofilaria immitis, Dirofilariasis, Enzyme-Linked Immunosorbent Assay, Female, Humans, Lung Diseases, Parasitic, Male, Middle Aged, Sensitivity and Specificity, Blotting, Western, Reproducibility of Results, Case-Control Studies
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