Feline dirofilariosis: antibody response to antigenic fractions containing specific 20 to 30 kDa polypeptides from the adult Dirofilaria immitis somatic antigen
Abstract
Fractions from the adult somatic antigen (SA) Dirofilaria immitis complex, containing polypeptides from 20 to 30kDa, previously identified as molecular markers of feline dirofilariosis are isolated by sequential application of gel filtration and anion exchange chromatography. Indirect enzyme-linked immunosorbent assays, employing these fractions (20-26kDa/ELISAF1 and 30kDa/ELISAF7) show multivalent diagnostic capacities: they were able to detect pre-patent infections 2 months after infection, infections in clinical phase, and the fall of antibodies after the worms were removed from the heart, or the application of a ivermectin treatment. The results obtained by the two tests correlated well, in spite of the fact that ELISAF1 was most useful to detect antibodies in sera from cats in the clinical phase, while ELISAF7 has more sensitivity for the early detection of the infections. Both ELISAs were useful in the detection of the decrease of antibodies after the worms were removed by surgery or pharmacological treatment.
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Immune response in feline cardiopulmonary dirofilariasis is generally strong after approximately 2 m.p.i., enabling early detection of single-worm infections.
“The immune response in feline cardiopulmonary dirofilariasis is generally strong after approximately 2 m.p.i., thereby enabling the early detection of single-worm infections (346,348).”
From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [346]
Cats with heartworm disease elicit a strong IgG response against D. immitis and Wolbachia.
“A strong IgG response against D. immitis and Wolbachia in cats with heartworm disease is also elicited (38,346).”
From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [346]
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Human and animal dirofilariasis: The emergence of a zoonotic mosaic
Simón, F., Siles-Lucas, M., Morchón, R., González-Miguel, J., Mellado, I., Carretón, E., & Montoya-Alonso, J. A. (2012) · Clinical Microbiology Reviews · doi:10.1128/cmr.00012-12 · Cited by 834 · see citing works
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- Authors
- Prieto, G., Ceciliani, F., Venco, L., Morchón, R., & Simón, F.
- Affiliations
- Universidad de Salamanca (ES)
- University of Milan (IT)
- Corresponding author
- Simón, F
- Venue
- Veterinary Parasitology
- Publisher
- Elsevier BV
- Published
- 2002-02
- Volume
- 103
- Issue
- 4
- Pages
- 341-353
- ISSN
- 0304-4017 (ISSN-L)
- OpenAlex
- W2092242870
- Cited by
- 11
- References
- 14
- Citations by year
- 2022: 2 · 2021: 1 · 2020: 1 · 2018: 1 · 2012: 1
- FWCI
- 0.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Veterinary Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Keywords
- Dirofilaria immitis, antibody response, somatic antigens, antigenic fractions, ELISA diagnostic test, ivermectin
- MeSH terms
- Animals, Anthelmintics, Antibodies, Helminth, Epitopes, Antigens, Helminth, Cat Diseases, Cats, Chromatography, Gel, Chromatography, Ion Exchange, Dirofilaria immitis, Dirofilariasis, Enzyme-Linked Immunosorbent Assay, Heart, Immunoglobulin G, Ivermectin, Molecular Weight
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