The DOI matches its Crossref record; 194 of the 194 references it cites were found in a registry or an index.
Episomal vectors for gene expression in mammalian cells
Abstract
An important reason for preferring mammalian cells for heterologous gene expression is their ability to make authentic proteins containing post‐translational modifications similar to those of the native protein. The development of expression systems for mammalian cells has been ongoing for several years, resulting in a wide variety of effective expression vectors. The aim of this review is to highlight episomal expression vectors. Such episomal plasmids are usually based on sequences from DNA viruses, such as BK virus, bovine papilloma virus 1 and Epstein–Barr virus. In this review we will mainly focus on the improvements made towards the usefulness of these systems for gene expression studies and gene therapy.
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“adeno-associated virus (AAV) vectors have shown promise as a gene therapy technology due to their effectiveness and ability to sustain gene expression for several years in certain IRDs [239,240,241].”
From Bruch’s Membrane: A Key Consideration with Complement-Based Therapies for Age-Related Macular Degeneration (2023) · cites this paper as [240]
Citations
Cites
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Lee, C. S., Bishop, E. S., Zhang, R., Yu, X., Farina, E. M., Yan, S., Zhao, C., Zeng, Z., Shu, Y., Wu, X., et al. (2017) · Genes & Diseases · doi:10.1016/j.gendis.2017.04.001 · Cited by 691
Lentiviral vectors for ocular gene therapy
Arsenijevic, Y., Berger, A., Udry, F., & Kostic, C. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14081605 · Cited by 47
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Metadata
- Authors
- Van Craenenbroeck, K., Vanhoenacker, P., & Haegeman, G.
- Affiliations
- Vlaams Instituut voor Biotechnologie (BE)
- Corresponding author
- Haegeman, Guy
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 2000-09
- Volume
- 267
- Issue
- 18
- Pages
- 5665-5678
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W1591316509
- Cited by
- 147
- References
- 194
- Citations by year
- 2025: 4 · 2024: 4 · 2023: 4 · 2022: 5 · 2021: 6 · 2020: 3 · 2019: 6 · 2018: 6 · 2017: 12 · 2016: 7 · 2015: 11 · 2014: 7 · 2013: 6 · 2012: 5
- FWCI
- 0.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- episomal vectors, mammalian cell expression systems, heterologous expression, BK virus, bovine papillomavirus 1, Epstein-Barr virus, post-translational modification, gene therapy
- MeSH terms
- Animals, BK Virus, Cell Line, Herpesvirus 4, Human, Genetic Vectors, Humans, Models, Genetic, Plasmids, Genetic Therapy, Gene Transfer Techniques, Transgenes, Bovine papillomavirus 1
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