The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
A subfraction of the yeast endoplasmic reticulum associates with the plasma membrane and has a high capacity to synthesize lipids
Abstract
Large parts of the endoplasmic reticulum of the yeast, Saccharomyces cerevisiae, are located close to intracellular organelles, i.e. mitochondria and the plasma membrane, as shown by fluorescence and electron microscopy. Here we report the isolation and characterization of the subfraction of the endoplasmic reticulum that is closely associated with the plasma membrane. This plasma membrane associated membrane (PAM) is characterized by its high capacity to synthesize phosphatidylserine and phosphatidylinositol. As such, PAM is reminiscent of MAM, a mitochondria associated membrane fraction of the yeast [Gaigg, B., Simbeni, R., Hrastnik, C., Paltauf, F. & Daum, G. (1995) Biochim. Biophys. Acta1234, 214–220], although the specific activity of phosphatidylserine synthase and phosphatidylinositol synthase in PAM exceeds several‐fold the activity in MAM and also in the bulk endoplasmic reticulum. In addition, several enzymes involved in ergosterol biosynthesis, namely squalene synthase (Erg9p), squalene epoxidase (Erg1p) and sterolδ 24‐methyltransferase (Erg6p), are highly enriched in PAM. A possible role of PAM in the supply of lipids to the plasma membrane is discussed.
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Osh4p exchanges sterols for phosphatidylinositol 4-phosphate between lipid bilayers
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Metadata
- Authors
- Pichler, H., Gaigg, B., Hrastnik, C., Achleitner, G., Kohlwein, S. D., Zellnig, G., Perktold, A., & Daum, G.
- Venue
- European Journal of Biochemistry
- Publisher
- Wiley
- Published
- 2001-04-15
- Volume
- 268
- Issue
- 8
- Pages
- 2351-2361
- ISSN
- 0014-2956 (ISSN-L)
- 1432-1033
- OpenAlex
- W1519919607
- Cited by
- 259
- References
- 38
- FWCI
- 4.19
- Type
- journal article
- Language
- en
- Open access
- closed
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- Pichler, H., Gaigg, B., Hrastnik, C., Achleitner, G., Kohlwein, S. D., Zellnig, G., Perktold, A., & Daum, G. (2001). A subfraction of the yeast endoplasmic reticulum associates with the plasma membrane and has a high capacity to synthesize lipids. European Journal of Biochemistry. https://doi.org/10.1046/j.1432-1327.2001.02116.x
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