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Cited by 14Photodynamic Therapy Research StudiesBladder and Urothelial Cancer TreatmentsUrinary Bladder and Prostate Research

The effect of photodynamic therapy on rat urinary bladder with orthotopic urothelial carcinoma

Grönlund‐Pakkanen, S., Wahlfors, J., Talja, M., Kosma, V.‐M., Pakkanen, T.M., Ala‐Opas, M., Alhava, E., Moore, R.B.·2003·BJU International
DOI 10.1046/j.1464-410x.2003.04290.x
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Abstract

OBJECTIVE: To assess the effect of whole‐bladder photodynamic therapy (PDT) on a rat model with orthotopic superficial bladder cancer, as PDT is an alternative intravesical therapy for treating superficial bladder cancer, based on an interaction between a photosensitizer and light energy to induce oxygen radicals that destroy tissue by lipid peroxidation. MATERIALS AND METHODS: In all, 76 female Fischer F344 rats were inoculated intravesically with AY‐27 tumour cells. After establishing superficial tumour, 24 rats were treated with PDT using aminolaevulinic acid (ALA)‐induced protoporphyrin IX as a photosensitizer, and a continuous‐wave argon pumped‐dye laser (638 nm). At 4 h after intravenous (300 mg/kg) or intravesical (100 mg/mL) administration of ALA the bladders were intravesically exposed to a 40 J/cm 2 light dose; 12 rats received no ALA but were exposed to the same light dose. Before administering ALA, urine cytology samples were taken for analysis. At 3 or 21 days the treated rats were killed and morphological changes in the bladder walls analysed by light microscopy. Forty rats served as controls to examine the presence of tumour. RESULTS: The tumour established in 33 of 40 rats (83%) in the controls, but after PDT with intravesical ALA there was carcinoma in only in one of 12 ( P < 0.001, Pearson's χ 2 test). After PDT with intravenous ALA there was carcinoma in five of 11 rats ( P = 0.063, Pearson's χ 2 test). In the control group of 12 rats receiving only light energy there was carcinoma in three ( P = 0.001, Pearson's χ 2 test). Histologically, at 3 days after PDT there was only mild superficial damage in all six rats treated intravesically. Bladder wall destruction reached the muscular layer, with an abscess in one of six rats treated intravenously. After 3 weeks of PDT there was muscular necrosis with perforation and abscess from catheterization two of six rats treated intravesically and in three the bladder wall totally recovered. In the intravenous group the bladder walls were normal or had only mild superficial damage. Cytology of the urine sediment failed to detect half the tumours in the treatment groups. CONCLUSION: These results support the use of PDT with intravesical ALA‐induced protoporphyrin X for treating superficial bladder carcinoma. Intravesical was better than intravenous ALA in eradicating bladder carcinoma with PDT.

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  • Prolonged animal survival followed by photodynamic therapy using ALA was reported by several groups in various experimental models.

    “Prolonged animal survival followed by photodynamic therapy using ALA was reported by several groups in various experimental models.”

    From Aminolevulinic Acid (ALA) as a Prodrug in Photodynamic Therapy of Cancer (2011) · cites this paper as [105]

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Authors
Grönlund‐Pakkanen, S., Wahlfors, J., Talja, M., Kosma, V. ‐., Pakkanen, T. M., Ala‐Opas, M., Alhava, E., & Moore, R. B.
Affiliations
  • Päijät-Hämeen Keskussairaala (FI)
  • Kuopio University Hospital (FI)
  • Helsinki University Hospital (FI)
  • University of Alberta (CA)
ORCID
Venue
BJU International
Publisher
Wiley
Published
2003-06-24
Volume
92
Issue
1
Pages
125-130
ISSN
  • 1464-4096 (ISSN-L)
  • 1464-410X
DOI
10.1046/j.1464-410x.2003.04290.x
OpenAlex
W1567438390
Cited by
14
References
32
Citations by year
2026: 1 · 2023: 1 · 2019: 1 · 2013: 2 · 2012: 1
FWCI
0.85
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • British Journal of Urology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Alberta Heritage Foundation for Medical Research
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
  • Health Sciences › Medicine › Surgery › Bladder and Urothelial Cancer Treatments
  • Health Sciences › Medicine › Urology › Urinary Bladder and Prostate Research
Keywords
photodynamic therapy, superficial bladder cancer, aminolaevulinic acid, protoporphyrin IX, photosensitizers, intravesical therapy, argon-pumped dye laser, tumor regression, rat model, urine cytology, cancer detection, lipid peroxidation
MeSH terms
Administration, Intravesical, Aminolevulinic Acid, Animals, Urinary Bladder, Urinary Bladder Neoplasms, Female, Infusions, Intravenous, Photochemotherapy, Protoporphyrins, Rats, Inbred F344, Photosensitizing Agents, Rats

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