Physiological evidence for a high‐affinity cadmium transporter highly expressed in a Thlaspi caerulescens ecotype
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• Uptake kinetics and translocation characteristics of cadmium and zinc are presented for two contrasting ecotypes of the Cd/Zn hyperaccumulator Thlaspi caerulescens, Ganges (southern France) and Prayon (Belgium). • Experiments using radioactive isotopes were designed to investigate the physiology of Cd and Zn uptake, and a pressure‐chamber system was employed to collect xylem sap. • In contrast to similar Zn uptake and translocation, measurements of concentration‐dependent influx of Cd revealed marked differences between ecotypes. Ganges alone showed a clear saturable component in the low Cd concentration range; maximum influx Vmax for Cd was fivefold higher in Ganges; and there was a fivefold difference in the Cd concentration in xylem sap. Addition of Zn to the uptake solution at equimolar concentration to Cd did not decrease Cd uptake by Ganges, but caused a 35% decrease in Prayon. • There is strong physiological evidence for a high‐affinity, highly expressed Cd transporter in the root cell plasma membranes of the Ganges ecotype of T. caerulescens. This raises evolutionary questions about specific transporters for non‐essential metals. The results also show the considerable scope for selecting hyperaccumulator ecotypes to achieve higher phytoextraction efficiencies.
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Cd uptake is not influenced by Zn concentration, indicating the presence of an efficient cadmium-specific transport system in the roots of this ecotype.
“Cd uptake is not influenced by Zn concentration, indicating the presence of an efficient cadmium-specific transport system in the roots of this ecotype.”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Lombi et al., 2001
The Results passage on Zn competition reports that Zn did not affect Cd uptake in the Ganges ecotype when both were at 10 µM, and the Discussion reports physiological evidence for a high-affinity Cd transporter in its roots. But at 0.2 µM Cd, adding 10 µM Zn significantly reduced apparent Cd uptake in Ganges by 49%, so the unqualified statement that Zn concentration does not influence uptake overstates the finding. (LLM, conf=0.91)
Experimental — accuracy not yet measuredThe Ganges ecotype of T. caerulescens could accumulate a very high level of Cd in their aerial parts.
“Interestingly, (Lombi et al., 2001) reported that the Ganges ecotype of T. caerulescens could accumulate a very high level of Cd in their aerial parts.”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Lombi et al., 2001
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- Authors
- Lombi, E., Zhao, F. J., McGrath, S. P., Young, S. D., & Sacchi, G. A.
- Affiliations
- Rothamsted Research (GB)
- University of Nottingham (GB)
- University of Milan (IT)
- Corresponding author
- McGrath, S. P.
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2001-01
- Volume
- 149
- Issue
- 1
- Pages
- 53-60
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- OpenAlex
- W2078654348
- Semantic Scholar
- e20624fc976b7a201bf0fa25b201e1e567eb2100
- Cited by
- 268
- References
- 26
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 2 · 2021: 10 · 2020: 7 · 2019: 7 · 2018: 5 · 2017: 12 · 2016: 7 · 2015: 9 · 2014: 7 · 2013: 14 · 2012: 16
- FWCI
- 23.07
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- New Phytologist (publishedVersion)
- PubMed (publishedVersion)
- Rothamsted Repository (Rothamsted Repository) (submittedVersion)
- Funders
- European Commission
- Directorate for Biological Sciences
- Biotechnology and Biological Sciences Research Council (BBS/E/C/00004174)
- Topics
- Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
- Physical Sciences › Chemistry › Analytical Chemistry › Heavy Metals in Plants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Keywords
- Thlaspi caerulescens, cadmium hyperaccumulation, zinc uptake, cadmium transporters, ecotypic variation, cadmium translocation, metal uptake kinetics, phytoextraction
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