Expression of the Glucagon‐Like Peptide‐1 Receptor Gene in Rat Brain
Abstract
Abstract: Evidence that glucagon‐like peptide‐1 (GLP‐1) (7–36) amide functions as a novel neuropeptide prompted us to study the gene expression of its receptor in rat brain. Northern blot analysis showed transcripts of similar size in RINm5F cells, hypothalamus, and brainstem. First‐strand cDNA was prepared by using RNA from hypothalamus, brainstem, and RINm5F cells and subsequently amplified by PCR. Southern blot analysis of the PCR products showed a major 1.4‐kb band in all these preparations. PCR products amplified from hypothalamus were cloned, and the nucleotide sequence of one strand was identical to that described in rat pancreatic islets. In situ hybridization studies showed specific labeling in both neurons and glia of the thalamus, hypothalamus, hippocampus, primary olfatory cortex, choroid plexus, and pituitary gland. In the hypothalamus, ventromedial nuclei cells were highly labeled. These findings indicate that GLP‐1 receptors are actually synthesized in rat brain. In addition, the colocalization of GLP‐1 receptors, glucokinase, and GLUT‐2 in the same areas supports the idea that these cells play an important role in glucose sensing in the brain.
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GLP-1 acts through GLP-1R, which is mainly expressed in pancreatic islets, the brain, and the GI tract, and less prominently in the kidney and eyes.
“GLP-1 acts through a class 2 member of the G-coupled protein C receptor family seven-transmembrane receptor (GLP-1R) (8), which is mainly expressed in pancreatic islets, the brain, and the gastrointestinal (GI) tract (9–12), and less prominently in the kidney and eyes (11–13).”
From The science of safety: adverse effects of GLP-1 receptor agonists as glucose-lowering and obesity medications (2026) · cites this paper as [10]
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- Authors
- Alvarez, E., Roncero, I., Chowen, J. A., Thorens, B., & Blázquez, E.
- Affiliations
- Universidad Complutense de Madrid (ES)
- Instituto Cajal (ES)
- University of Lausanne (CH)
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 1996-03
- Volume
- 66
- Issue
- 3
- Pages
- 920-927
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W2000115601
- Cited by
- 194
- References
- 0
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 6 · 2023: 13 · 2022: 7 · 2021: 10 · 2020: 9 · 2019: 6 · 2018: 4 · 2017: 4 · 2016: 4 · 2015: 5 · 2014: 10 · 2013: 8 · 2012: 11
- FWCI
- 2.19
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Neurochemistry (publishedVersion)
- PubMed (publishedVersion)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
- Keywords
- GLP-1 receptor, neuropeptides, hypothalamus, brainstem, thalamus, hippocampus, choroid plexus, pituitary gland, glucose detection, glucokinase, GLUT2, receptor colocalization, rat brain
- MeSH terms
- Glucagon-Like Peptide-1 Receptor, Animals, Base Sequence, Brain, Male, Molecular Sequence Data, RNA, Messenger, Blotting, Southern, Blotting, Northern, Molecular Probes, Gene Expression, Polymerase Chain Reaction, Rats, Wistar, In Situ Hybridization, Receptors, Glucagon, Mice, Rats
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