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Expression of the Na+‐d‐Glucose Cotransporter SGLT1 in Neurons

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Poppe, Robert, Karbach, Ulrich, Gambaryan, Stepan, Wiesinger, Heinrich, Lutzenburg, Michael, Kraemer, Matthias, Witte, Otto W., Koepsell, Hermann·1997·Journal of Neurochemistry
DOI 10.1046/j.1471-4159.1997.69010084.x
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Abstract

Abstract: In brains of the rabbit, pig, and human, expression of the high‐affinity Na+‐d‐glucose cotransporter SGLT1 and of the protein RS1, which alters the activity of SGLT1, was demonstrated. In situ hybridization showed that SGLT1 and RS1 are transcribed in pyramidal cells of brain cortex and hippocampus and in Purkinje cells of cerebellum. In neurons of pig brain SGLT1 protein was demonstrated by western blotting with synaptosomal membranes and by immunohistochemistry, which showed SGLT1 in pyramidal and Purkinje cells. To test whether SGLT1 in neurons may be activated during increased d‐glucose consumption, an epileptic seizure was induced in rat brain, and the uptake of specific nonmetabolized substrates of SGLT1 {[14 C]methyl‐α‐d‐glucopyranoside ([14 C]AMG)} and of Na+‐independent transporters {2‐deoxy‐d‐[14 C]glucose([14 C]2‐DG)} was analyzed by autoradiography. During the seizure the uptake of AMG and 2‐DG was increased in the focus. Within two hours after the seizure 2‐DG uptake in the focus returned to normal. In contrast, the AMG uptake in the focus area was still increased 1 day later. The data show that the high‐affinity Na+‐d‐glucose cotransporter SGLT1 is expressed in neurons and can be up‐regulated.

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  • SGLT1 is present in pyramidal cells of the brain cortex, Purkinje cerebellum cells, hippocampus pyramidal and granular cells.

    “SGLT1 inhibitors are present in the pyramidal cells of the brain cortex, Purkinje cerebellum cells, hippocampus pyramidal, and granular cells [20].”

    From Neuroprotective effect of SGLT2 inhibitors. (2021) · cites this paper as [20]

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Authors
Poppe, R., Karbach, U., Gambaryan, S., Wiesinger, H., Lutzenburg, M., Kraemer, M., Witte, O. W., & Koepsell, H.
Affiliations
  • University of Würzburg (DE)
  • University of Tübingen (DE)
  • Heinrich Heine University Düsseldorf (DE)
Corresponding author
Koepsell, Hermann
ORCID
Venue
Journal of Neurochemistry
Publisher
Wiley
Published
1997-07
Volume
69
Issue
1
Pages
84-94
ISSN
  • 0022-3042 (ISSN-L)
  • 1471-4159
DOI
10.1046/j.1471-4159.1997.69010084.x
OpenAlex
W1978754654
Cited by
134
References
0
Citations by year
2026: 2 · 2025: 7 · 2024: 10 · 2023: 10 · 2022: 8 · 2021: 5 · 2020: 8 · 2019: 3 · 2018: 7 · 2017: 6 · 2016: 5 · 2015: 3 · 2014: 5 · 2013: 3 · 2012: 4
FWCI
1.07
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 1966 d)
Locations
  • Journal of Neurochemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Keywords
SGLT1, RS1, neuronal glucose uptake, pyramidal neurons, Purkinje cells, epileptic seizures, cerebral cortex, hippocampus, cerebellum, synaptosomal membranes
MeSH terms
Animals, Brain Chemistry, Carbon Radioisotopes, Deoxyglucose, Dogs, Epilepsy, Glucose, Humans, Membrane Glycoproteins, Molecular Sequence Data, Monosaccharide Transport Proteins, Neuroglia, Neurons, Rabbits, Sodium, Swine, Transcription, Genetic, Blotting, Western, Up-Regulation, Gene Expression, In Situ Hybridization, Sequence Analysis, DNA, DNA, Complementary, Sodium-Glucose Transporter 1, Rats

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