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Revised immunolocalization of the Na+-d-glucose cotransporter SGLT1 in rat organs with an improved antibody

Balen, Daniela, Ljubojević, Marija, Breljak, Davorka, Brzica, Hrvoje, Z̆lender, Vilim, Koepsell, Hermann, Sabolić, Ivan·2008·American Journal of Physiology-Cell Physiology
DOI 10.1152/ajpcell.00180.2008
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Abstract

Previously, we characterized localization of Na+-glucose cotransporter SGLT1 ( Slc5a1) in the rat kidney using a polyclonal antibody against the synthetic COOH-terminal peptide of the rat protein (Sabolić I, Škarica M, Gorboulev V, Ljubojević M, Balen D, Herak-Kramberger CM, Koepsell H. Am J Physiol Renal Physiol 290: 913–926, 2006). However, the antibody gave some false-positive reactions in immunochemical studies. Using a shortened peptide for immunization, we have presently generated an improved, more specific anti-rat SGLT1 antibody (rSGLT1-ab), which in immunochemical studies with isolated membranes and tissue cryosections from male (M) and female (F) rats exhibited 1) in kidneys and small intestine, labeling of a major protein band of ∼75 kDa; 2) in kidneys of adult animals, localization of rSGLT1 to the proximal tubule (PT) brush-border membrane (S1 < S2 < S3) and intracellular organelles (S1 > S2 > S3), with zonal (cortex < outer stripe) and sex differences (M < F) in the protein expression, which correlated well with the tissue expression of its mRNA in RT-PCR studies; 3) in kidneys of castrated adult M rats, upregulation of the protein expression; 4) in kidneys of prepubertal rats, weak and sex-independent labeling of the 75-kDa protein band and immunostaining intensity; 5) in small intestine, sex-independent regional differences in protein abundance (jejunum > duodenum = ileum); and 6) thus far unrecognized localization of the transporter in cortical thick ascending limbs of Henle and macula densa in kidney, bile ducts in liver, enteroendocrine cells and myenteric plexus in the small intestine, and initial ducts in the submandibular gland. Our improved rSGLT1-ab may be used to identify novel sites of SGLT1 localization and thus unravel additional physiological functions of this transporter in rat organs.

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Crossref · 10.1152/ajpcell.00180.2008

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  • SGLT-1 is expressed primarily in the small intestine with highest density in the jejunum, followed by the duodenum and then the ileum.

    “SGLT-1 is expressed primarily in the small intestine with highest density in the jejunum followed by the duodenum and then ileum (39,40).”

    From Gut mechanisms linking intestinal sweet sensing to glycemic control (2018) · cites this paper as [39]

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Authors
Balen, D., Ljubojević, M., Breljak, D., Brzica, H., Z̆lender, V., Koepsell, H., & Sabolić, I.
Affiliations
Institute for Medical Research and Occupational Health (HR)
ORCID
Venue
American Journal of Physiology-Cell Physiology
Publisher
American Physiological Society
Published
2008-08
Volume
295
Issue
2
Pages
C475-C489
ISSN
  • 0363-6143 (ISSN-L)
  • 1522-1563
DOI
10.1152/ajpcell.00180.2008
OpenAlex
W2113687888
Cited by
166
References
61
Citations by year
2026: 4 · 2025: 7 · 2024: 8 · 2023: 8 · 2022: 6 · 2021: 10 · 2020: 15 · 2019: 13 · 2018: 11 · 2017: 8 · 2016: 8 · 2015: 10 · 2014: 11 · 2013: 7 · 2012: 9
FWCI
4.25
Type
journal article
Language
en
Open access
closed
Locations
  • American Journal of Physiology-Cell Physiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
U.S. National Library of Medicine
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords
SGLT1, Na+/glucose cotransporter, antibody specificity, proximal tubule, brush border membrane, sex differences, castration, prepubertal development, small intestine, loop of Henle, macula densa, bile ducts, enteroendocrine cells, myenteric plexus, submandibular gland, kidney, rats
MeSH terms
Animals, Antibodies, Brain, Castration, Cell Membrane, Colon, Female, Gastric Mucosa, Immunohistochemistry, Intestine, Small, Jejunum, Kidney, Kidney Tubules, Proximal, Liver, Male, Microvilli, Nephrons, Ovariectomy, Salivary Glands, Sex Factors, Stomach, Blotting, Western, Gene Expression, Rats, Wistar, Sodium-Glucose Transporter 1, Rats

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