Enhanced neuronal protection from oxidative stress by coculture with glutamic acid decarboxylase‐expressing astrocytes

Lamigeon, C.; Bellier, J. P.; Sacchettoni, S.; Rujano, M.; Jacquemont, B.

doi:10.1046/j.1471-4159.2001.00278.x

Cited by 41Neuroscience and Neuropharmacology ResearchRedox biology and oxidative stressNeurological Disorders and Treatments

Enhanced neuronal protection from oxidative stress by coculture with glutamic acid decarboxylase‐expressing astrocytes

Open access

Lamigeon, C., Bellier, J. P., Sacchettoni, S., Rujano, M., Jacquemont, B.·2001·Journal of Neurochemistry

DOI 10.1046/j.1471-4159.2001.00278.x

Open this paper in CoChat — free account Open PDF

Abstract

Astrocytes expressing glutamic acid decarboxylase GAD67 directed by the glial fibrillary acidic protein promoter were shown to provide enhanced protection of PC12 cells from H 2 O 2 treatment and serum deprivation in the presence of glutamate. In addition, they protected non‐differentiated, but not differentiated, embryonic rat cortical neurons from glutamate toxicity. Glutamic acid decarboxylase (GAD)‐expressing astrocytes showed increased glutathione synthesis and release compared to control astrocytes. These changes were due to GAD transgene expression, as transient expression of a GAD antisense plasmid resulted in partial suppression of the increase in glutathione release. In addition to the previously demonstrated increases in NADH and ATP levels and lactate release, GAD‐expressing astrocytes show increased antioxidant activity, explaining their ability to protect neurons from various injuries.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1046/j.1471-4159.2001.00278.x

Cites

69 of 69 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Several reports showed that enhanced GAD2 activity may contribute to neuronal protection from oxidative stress in vitro neuronal tissue cultures.
“In this way, several reports showed that enhanced GAD2 activity may contribute to neuronal protection from oxidative stress in vitro neuronal tissue cultures [238, 239].”
From Oxidative Stress Implications in the Affective Disorders: Main Biomarkers, Animal Models Relevance, Genetic Perspectives, and Antioxidant Approaches (2016) · cites this paper as [239]

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
Mosmann, T. (1983) · Journal of Immunological Methods · doi:10.1016/0022-1759(83)90303-4 · Cited by 56,104 · see citing works
Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues
Tietze, F. (1969) · Analytical Biochemistry · doi:10.1016/0003-2697(69)90064-5 · Cited by 6,077 · see citing works
Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Greene, L., & Tischler, A. S. (1976) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.73.7.2424 · Cited by 5,594 · see citing works
Oxidative Stress, Glutamate, and Neurodegenerative Disorders
Coyle, J. T., & Puttfarcken, P. (1993) · Science · doi:10.1126/science.7901908 · Cited by 3,762 · see citing works
Glutamate uptake into astrocytes stimulates aerobic glycolysis: a mechanism coupling neuronal activity to glucose utilization.
Pellerin, L., & Magistretti, P. J. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.22.10625 · Cited by 2,927 · see citing works
Aging, energy, and oxidative stress in neurodegenerative diseases
Beal, M. F. (1995) · Annals of Neurology · doi:10.1002/ana.410380304 · Cited by 1,434 · see citing works
Glutamate toxicity in a neuronal cell line involves inhibition of cystine transport leading to oxidative stress
Murphy, T. H., Miyamoto, M., Sastre, A., Schnaar, R. L., & COYLE, J. T. (1989) · Neuron · doi:10.1016/0896-6273(89)90043-3 · Cited by 1,033 · see citing works
A Dissection and tissue culture manual of the nervous system
Shahar, A. (1989) · Andalas University Repository (Andalas University) · Cited by 758 · see citing works
Reactive oxygen species and programmed cell death
Jacobson, M. D. (1996) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(96)20008-8 · Cited by 753 · see citing works
bcl-2 inhibits death of central neural cells induced by multiple agents.
Zhong, L. T., Sarafian, T. A., Kane, D. J., Charles, A. C., Mah, S. P., Edwards, R. H., & Bredesen, D. E. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.10.4533 · Cited by 619 · see citing works
Synthesis of the Antioxidant Glutathione in Neurons: Supply by Astrocytes of CysGly as Precursor for Neuronal Glutathione
Dringen, R., Pfeiffer, B., & Hamprecht, B. (1999) · The Journal of Neuroscience · doi:10.1523/jneurosci.19-02-00562.1999 · Cited by 589 · see citing works
Do defecs in mitochondrial energy metabolism underlie the pathology of neurodegenerative diseases?
Beal, M. F., Hyman, B. T., & Koroshetz, W. J. (1993) · Trends in Neurosciences · doi:10.1016/0166-2236(93)90117-5 · Cited by 574 · see citing works
Repression of gene expression by oxidative stress
Morel, Y., & Barouki, R. (1999) · Biochemical Journal · doi:10.1042/bj3420481 · Cited by 543 · see citing works
Astrocytes protect neurons from hydrogen peroxide toxicity
Desagher, S., Glowinski, J., & Prémont, J. (1996) · Journal of Neuroscience · doi:10.1523/jneurosci.16-08-02553.1996 · Cited by 543 · see citing works
Prospects for new restorative and neuroprotective treatments in Parkinson's disease
Dunnett, S. B., & Björklund, A. (1999) · Nature · doi:10.1038/399a032 · Cited by 539 · see citing works
Energetics in the pathogenesis of neurodegenerative diseases
Beal, M. F. (2000) · Trends in Neurosciences · doi:10.1016/s0166-2236(00)01584-8 · Cited by 472 · see citing works
Immature cortical neurons are uniquely sensitive to glutamate toxicity by inhibition of cystine uptake
Murphy, T. H., Schnaar, R. L., & Coyle, J. T. (1990) · The FASEB Journal · doi:10.1096/fasebj.4.6.2180770 · Cited by 428 · see citing works
Selective distribution of lactate dehydrogenase isoenzymes in neurons and astrocytes of human brain
Bittar, P. G., Charnay, Y., Pellerin, L., Bouras, C., & Magistretti, P. J. (1996) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/00004647-199611000-00001 · Cited by 423 · see citing works
Chapter 6 Glutamate receptors and the induction of excitotoxic neuronal death
Choi, D. W. (1994) · Progress in Brain Research · doi:10.1016/s0079-6123(08)60767-0 · Cited by 385 · see citing works
Calcium and reactive oxygen species mediate staurosporine-induced mitochondrial dysfunction and apoptosis in PC12 cells
Kruman, I. I., Guo, Q., & Mattson, M. P. (1998) · Journal of Neuroscience Research · doi:10.1002/(sici)1097-4547(19980201)51:3 · Cited by 377 · see citing works
Pyruvate Protects Neurons against Hydrogen Peroxide-Induced Toxicity
Desagher, S., Glowinski, J., & Prémont, J. (1997) · Journal of Neuroscience · doi:10.1523/jneurosci.17-23-09060.1997 · Cited by 370 · see citing works
An electron microscopic study of the development of axons and dendrites by hippocampal neurons in culture. I. Cells which develop without intercellular contacts
Bartlett, W., & Banker, G. (1984) · Journal of Neuroscience · doi:10.1523/jneurosci.04-08-01944.1984 · Cited by 348 · see citing works
The Role of Monoamine Metabolism in Oxidative Glutamate Toxicity
Maher, P. A., & Davis, J. B. (1996) · Journal of Neuroscience · doi:10.1523/jneurosci.16-20-06394.1996 · Cited by 338 · see citing works
The operation of the γ-aminobutyrate bypath of the tricarboxylic acid cycle in brain tissue in vitro
Hammond, B. J., Balázs, R., Machiyama, Y., Julian, T., & Richter, D. (1970) · Biochemical Journal · doi:10.1042/bj1160445 · Cited by 317 · see citing works
Overexpression of Bcl-2 with herpes simplex virus vectors protects CNS neurons against neurological insults in vitro and in vivo
Lawrence, M., Ho, D., Sun, G., et al. (1996) · Journal of Neuroscience · doi:10.1523/jneurosci.16-02-00486.1996 · Cited by 307 · see citing works
Growth Factors Prevent Mitochondrial Dysfunction, Loss of Calcium Homeostasis, and Cell Injury, but Not ATP Depletion in Hippocampal Neurons Deprived of Glucose
Mattson, M. P., Zhang, Y., & Bose, S. C. (1993) · Experimental Neurology · doi:10.1006/exnr.1993.1066 · Cited by 265 · see citing works
Antioxidants protect against glutamate-induced cytotoxicity in a neuronal cell line.
Miyamoto, M., Murphy, T. H., Schnaar, R. L., & Coyle, J. T. (1989) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)22783-5 · Cited by 247 · see citing works
Shift of the Cellular Oxidation‐Reduction Potential in Neural Cells Expressing Bcl‐2
Ellerby, L., Ellerby, H. M., Park, S. M., Holleran, A. L., Murphy, A. N., Fiskum, G. M., Kane, D. J., Testa, M. P., Kayalar, Ç., & Bredesen, D. E. (1996) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1996.67031259.x · Cited by 223 · see citing works
PHARMACOLOGY OF NEUROTROPHIC FACTORS
Hefti, F. F. (1997) · The Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.37.1.239 · Cited by 186 · see citing works
Regulation of GABA Level in Rat Brain Synaptosomes: Fluxes Through Enzymes of the GABA Shunt and Effects of Glutamate, Calcium, and Ketone Bodies
Erecińska, M., NELSON, D. S., Daikhin, Y., & Yudkoff, M. (1996) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1996.67062325.x · Cited by 179 · see citing works
Glial Cells Protect Neurons Against Oxidative Stress via Transcriptional Up‐Regulation of the Glutathione Synthesis
Iwata‐Ichikawa, E., Kondo, Y., Miyazaki, I., Asanuma, M., & Ogawa, N. (1999) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1999.0722334.x · Cited by 173 · see citing works
Glutathione Content as an Indicator for the Presence of Metabolic Pathways of Amino Acids in Astroglial Cultures
Dringen, R., & Hamprecht, B. (1996) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1996.67041375.x · Cited by 164 · see citing works
From entropy to Duino
Bignami, G. F. (1999) · Nature · doi:10.1038/19899 · Cited by 163 · see citing works
Oxidative glutamate toxicity involves mitochondrial dysfunction and perturbation of intracellular Ca2+ homeostasis
Pereira, C., & Oliveira, C. R. (2000) · Neuroscience Research · doi:10.1016/s0168-0102(00)00124-3 · Cited by 156 · see citing works
Glutathione Turnover in Cultured Astrocytes: Studies with [15N]Glutamate
Yudkoff, M., Pleasure, D., Cregar, L., Lin, Z., Nissim, I. B., Stern, J., & Nissim, I. (1990) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1990.tb08831.x · Cited by 156 · see citing works
Depolarization of In Situ Mitochondria Due to Hydrogen Peroxide‐Induced Oxidative Stress in Nerve Terminals
Chinopoulos, C., Tretter, L., & Ádám‐Vizi, V. (1999) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1999.0730220.x · Cited by 154 · see citing works
Genetically modified cells: applications for intracerebral grafting
Gage, F. H., Kawaja, M. D., & Fisher, L. J. (1991) · Trends in Neurosciences · doi:10.1016/0166-2236(91)90156-o · Cited by 148 · see citing works
Role of Nerve Growth Factor in Oxidanl Homeostasis: Glutathione Metabolism
Pan, Z., & Perez‐Polo, R. J. (1993) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1993.tb09808.x · Cited by 145 · see citing works
Oligodendrocytes utilize a matrix metalloproteinase, MMP-9, to extend processes along an astrocyte extracellular matrix
Uhm, J. H., Dooley, N. P., Oh, L. Y. S., & Yong, V. W. (1998) · Glia · doi:10.1002/(sici)1098-1136(199801)22:1 · Cited by 144 · see citing works
Enhanced Oxidative Stress and Altered Antioxidants in Brains of Bcl‐2‐Deficient Mice
Hochman, A., Sternin, H., Gorodin, S., et al. (1998) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1998.71020741.x · Cited by 140 · see citing works
Glucocorticoids Exacerbate Hypoxic and Hypoglycemic Hippocampal Injury In Vitro: Biochemical Correlates and a Role for Astrocytes
Tombaugh, G. C., Yang, S. H., Swanson, R. A., & Sapolsky, R. M. (1992) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1992.tb08884.x · Cited by 134 · see citing works
Increased transcription of the astrocyte gene GFAP during middle-age is attenuated by food restriction: Implications for the role of oxidative stress
Morgan, T. E., Rozovsky, I., Goldsmith, S. K., Stone, D. J., Yoshida, T., & Finch, C. E. (1997) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(97)00120-2 · Cited by 126 · see citing works
Cystine/glutamate antiporter expression in retinal mu¨ller glial cells: Implications fordl-alpha-aminoadipate toxicity
Kato, S., Ishita, S., Sugawara, K., & Mawatari, K. (1993) · Neuroscience · doi:10.1016/0306-4522(93)90080-y · Cited by 119 · see citing works
Glutamate Toxicity on a PC12 Cell Line Involves Glutathione (GSH) Depletion and Oxidative Stress
Pereira, C., & Oliveira, C. R. (1997) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(97)00020-8 · Cited by 115 · see citing works
The regeneration of reduced glutathione in rat forebrain mitochondria identifies metabolic pathways providing the NADPH required
Vogel, R., Wiesinger, H., Hamprecht, B., & Dringen, R. (1999) · Neuroscience Letters · doi:10.1016/s0304-3940(99)00748-x · Cited by 115 · see citing works
The Level of GAD67 Protein Is Highly Sensitive to Small Increases in Intraneuronal γ‐Aminobutyric Acid Levels
Rimvall, K., & Martin, D. L. (1994) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1994.62041375.x · Cited by 109 · see citing works
Astrocytic cell clones derived from established cultures of 8-day postnatal mouse cerebella
Alliot, F., & Pessac, B. (1984) · Brain Research · doi:10.1016/0006-8993(84)90377-9 · Cited by 108 · see citing works
Transplantation of glial cells into the CNS
Blakemore, W. F., & Franklin, R. J. M. (1991) · Trends in Neurosciences · doi:10.1016/0166-2236(91)90155-n · Cited by 105 · see citing works
Vulnerability to glucose deprivation injury correlates with glutathione levels in astrocytes
Papadopoulos, M. C., Koumenis, I. L., Dugan, L. L., & Giffard, R. G. (1997) · Brain Research · doi:10.1016/s0006-8993(96)01293-0 · Cited by 105 · see citing works
Glutathione Restoration as Indicator for Cellular Metabolism of Astroglial Cells
Dringen, R., & Hamprecht, B. (1998) · Developmental Neuroscience · doi:10.1159/000017337 · Cited by 105 · see citing works
Two Forms of Rat Brain Glutamic Acid Decarboxylase Differ in Their Dependence on Free Pyridoxal Phosphate
Denner, L. A., & Wu, J. (1985) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1985.tb12910.x · Cited by 94 · see citing works
Glial Formation of Pyruvate and Lactate from TCA Cycle Intermediates: Implications for the Inactivation of Transmitter Amino Acids?
Hassel, B., & Sonnewald, U. (1995) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1995.65052227.x · Cited by 87 · see citing works
BCL‐2‐Related Protein Expression in Apoptosis: Oxidative Stress Versus Serum Deprivation in PC12 Cells
Maroto, R., & Perez-Polo, J. R. (1997) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1997.69020514.x · Cited by 86 · see citing works
Neuroprotective mechanism of glial cell line-derived neurotrophic factor on dopamine neurons: role of antioxidation
Chao, C.-C., & Lee, E. H. Y. (1999) · Neuropharmacology · doi:10.1016/s0028-3908(99)00030-1 · Cited by 84 · see citing works
Potentiation of murine astrocyte antioxidant defence by bcl‐2: protection in part reflects elevated glutathione levels
Papadopoulos, M. C., Koumenis, I. L., Xu, L., et al. (1998) · European Journal of Neuroscience · doi:10.1046/j.1460-9568.1998.00134.x · Cited by 65 · see citing works
Reactive oxygen species involved in the glutamate toxicity of C6 glioma cells via XC¯ antiporter system
Mawatari, K., Yasui, Y., Sugitani, K., Takadera, T., & Kato, S. (1996) · Neuroscience · doi:10.1016/0306-4522(96)00025-5 · Cited by 60 · see citing works
Different mechanisms of glutamate-induced neuronal death between dopaminergic and non-dopaminergic neurons in rat mesencephalic culture
Sawada, H., Kawamura, T., Shimohama, S., AKAIKE, A., & Kimura, J. (1996) · Journal of Neuroscience Research · doi:10.1002/(sici)1097-4547(19960215)43:4 · Cited by 52 · see citing works
Neurotrophin Regulation of Energy Homeostasis in the Central Nervous System
Jackson, G. R., Werrbach‐Perez, K., Pan, Z., et al. (2007) · Developmental Neuroscience · doi:10.1159/000112121 · Cited by 49 · see citing works
Regulation of Antioxidant Enzyme Expression by NGF
Sampath, D., & Perez‐Polo, R. J. (1997) · Neurochemical Research · doi:10.1023/a:1027387105882 · Cited by 46 · see citing works
Protection against hydrogen peroxide cytotoxicity in Rat-1 fibroblasts provided by the oncoprotein Bcl-2: maintenance of calcium homoeostasis is secondary to the effect of Bcl-2 on cellular glutathione
RIMPLER, M. M., Rauen, U., SCHMIDT, T., et al. (1999) · Biochemical Journal · doi:10.1042/bj3400291 · Cited by 45 · see citing works
Oxidant Injury in PC12 Cells—A Possible Model of Calcium “Dysregulation” in Aging: I. Selectivity of Protection Against Oxidative Stress
Joseph, J. A., Strain, J. G., Jimenez, N. D., et al. (1997) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1997.69031252.x · Cited by 44 · see citing works
Transaminations catalysed by brain glutamate decarboxylase
Porter, T., Spink, D. C., Martin, S. B., et al. (1985) · Biochemical Journal · doi:10.1042/bj2310705 · Cited by 41 · see citing works
Reversible and irreversible oxidant injury to PC12 cells by hydrogen peroxide
Halleck, M. M., Richburg, J. H., & Kauffman, F. C. (1992) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(92)90007-4 · Cited by 34 · see citing works
Identification of PSF, the polypyrimidine tract-binding protein-associated splicing factor, as a developmentally regulated neuronal protein
Chanas-Sacré, G., Mazy‐Servais, C., Wattiez, R., Pirard, S., Rogister, B., Patton, J. G., Belachew, S. M., Malgrange, B., Moonen, G., & Leprince, P. (1999) · Journal of Neuroscience Research · doi:10.1002/(sici)1097-4547(19990701)57:1 · Cited by 33 · see citing works
Protection against hydrogen peroxide cytotoxicity in Rat-1 fibroblasts provided by the oncoprotein Bcl-2: maintenance of calcium homoeostasis is secondary to the effect of Bcl-2 on cellular glutathione
RIMPLER, M. M., Rauen, U., SCHMIDT, T., et al. (1999) · Biochemical Journal · doi:10.1042/0264-6021:3400291 · Cited by 22 · see citing works
Glutathione protects against hypoxic/hypoglycemic decreases in 2-deoxyglucose uptake and presynaptic spikes in hippocampal slices
Shibata, S., Tominaga, K., & Watanabe, S. (1995) · European Journal of Pharmacology · doi:10.1016/0014-2999(94)00727-o · Cited by 15 · see citing works
Glutamic Acid Decarboxylase‐Expressing Astrocytes Exhibit Enhanced Energetic Metabolism and Increase PC12 Cell Survival Under Glucose Deprivation
Bellier, J., Sacchettoni, S., Prod'hon, C., et al. (2000) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2000.0750056.x · Cited by 9 · see citing works
Self-citation
Glucose transporter proteins in brain: Delivery of glucose to neurons and glia
Vannucci, S. J., Maher, F., & Simpson, I. A. (1997) · Glia · doi:10.1002/(sici)1098-1136(199709)21:1

Flagged first, then by citations

Sorted by citations

Oxidative Stress Implications in the Affective Disorders: Main Biomarkers, Animal Models Relevance, Genetic Perspectives, and Antioxidant Approaches
Balmus, I. M., Ciobica, A., Antioch, I., Dobrin, R., & Timofte, D. (2016) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2016/3975101 · Cited by 103 · see citing works
“In this way, several reports showed that enhanced GAD2 activity may contribute to neuronal protection from oxidative stress in vitro neuronal tissue cultures [238, 239].”
Background
Enhancement of neuronal protection from oxidative stress by glutamic acid decarboxylase delivery with a defective herpes simplex virus vector
Lamigeon, C., Prod'Hon, C., De Frias, V, Michoudet, C., & Jacquemont, B. (2003) · Experimental Neurology · doi:10.1016/s0014-4886(03)00400-x · Cited by 13 · see citing works
Self-citation

Fine distribution of γ-aminobutyric acid, glutamic acid decarboxylase, and glutamic acid in the rabbit cerebellum
Kanno, S., & Okada, Y. (1988) · Experimental Neurology · doi:10.1016/0014-4886(88)90193-8 · Cited by 1 · see citing works
Ammonia Induced Decrease in Glial Fibrillary Acidic Protein in Cultured Astrocytes
Norenberg, M. D., Neary, J. T., Norenberg, L.-O. B., et al. (1990) · Journal of Neuropathology & Experimental Neurology · doi:10.1097/00005072-199007000-00004 · Cited by 75 · see citing works
Observation of astrocyte proliferation and glial fibrillary acid protein expression for evaluating cerebral infarction
Yan-nan, F. (2002) · Zhongguo bingli shengli zazhi
Effects of prolonged ethanol exposure on the glial fibrillary acidic protein-containing intermediate filaments of astrocytes in primary culture: a quantitative immunofluorescence and immunogold electron microscopic study.
Renau‐Piqueras, J., Zaragozá, R., de Paz, P., Báguena-Cervellera, R., Megias, L. D. R., & Guerri, C. (1989) · Journal of Histochemistry & Cytochemistry · doi:10.1177/37.2.2642942 · Cited by 106 · see citing works
Alterations in the glial fibrillary acidic protein content of primary astrocyte cultures for evaluation of glial cell toxicity
Cookson, M., & Pentreath, V. W. (1994) · Toxicology in Vitro · doi:10.1016/0887-2333(94)90156-2 · Cited by 53 · see citing works

Authors: Lamigeon, C., Bellier, J. P., Sacchettoni, S., Rujano, M., & Jacquemont, B.
Affiliations: Inserm (FR)
Hospital Vargas (VE)
Corresponding author: Jacquemont, B.
ORCID: Bellier, J. P.
Rujano, M.
Venue: Journal of Neurochemistry
Publisher: Wiley
Published: 2001-04-15
Volume: 77
Issue: 2
Pages: 598-606
ISSN: 0022-3042 (ISSN-L)
1471-4159
DOI: 10.1046/j.1471-4159.2001.00278.x
OpenAlex: W1544500316
Cited by: 41
References: 69
Citations by year: 2024: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2016: 4 · 2015: 1 · 2014: 2 · 2013: 1 · 2012: 5
FWCI: 0.74
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor, 249 d)
Locations: Journal of Neurochemistry (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
Keywords: glutamic acid decarboxylase, GAD67, astrocytes, neuroprotection, oxidative stress, glutamate excitotoxicity, hydrogen peroxide, serum deprivation, PC12 cells, glutathione synthesis, glutathione release, antioxidant activity, glial fibrillary acidic protein promoter, lactate release, NADH, ATP, cortical neurons
MeSH terms: Animals, Astrocytes, Cell Differentiation, Energy Metabolism, Genes, Synthetic, Glial Fibrillary Acidic Protein, Glutamate Decarboxylase, Glutathione, Hydrogen Peroxide, Isoenzymes, Neoplasm Proteins, Nerve Tissue Proteins, Neurons, Oxidation-Reduction, Promoter Regions, Genetic, Recombinant Fusion Proteins, Transfection, PC12 Cells, Culture Media, Serum-Free, Oxidative Stress, Glutamic Acid, Coculture Techniques, Transgenes, Mice, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Enhanced neuronal protection from oxidative stress by coculture with glutamic acid decarboxylase‐expressing astrocytes

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Self-check

Cites

Cited by

Related

Record

About these checks