The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Activity of natural antimicrobial compounds against Escherichia coli and Salmonella enterica serovar Typhimurium
Abstract
AIMS: The objective of this study was to evaluate the inhibitory activity of several natural organic compounds alone or in combination with nisin against Escherichia coli and Salmonella Typhimurium. METHODS AND RESULTS: The minimum inhibitory concentration (MIC) of five natural organic compounds were determined, and the effect of their combinations with nisin was evaluated by the checkerboard assay using the Bioscreen C. As expected, nisin by itself showed no inhibition against either of the Gram-negative bacteria. Thymol was found to be the most effective with the lowest MIC values of 1.0 and 1.2 mmol 1-1 against Salm. Typhimurium and E. coli, respectively. After thymol, the antimicrobial order of the natural organic compounds was carvacrol > eugenol > cinnamic acid > diacetyl. However, the combination of nisin with the natural organic compounds did not result in the enhancement of their antimicrobial activities. On the contrary, combination of nisin with diacetyl against Salm. Typhimurium resulted in an antagonism of diacetyl activity. CONCLUSIONS: While the individual natural organic compounds showed inhibitory activity against the two Gram-negatives, their combinations with nisin showed no improvement of antimicrobial activity. SIGNIFICANCE AND IMPACT OF THE STUDY: This study shows the potential of the natural organic compounds to control E. coli and Salm. Typhimurium.
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“Diacetyl has been shown to exert antifungal and antibacterial effects at low pH [167,168].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [168]
Citations
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Cited by
Sorted by citations
Antibacterial and antifungal activities of thymol: A brief review of the literature
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“A report from Olasupo et al. (2003) revealed the antibacterial effect of thymol with the lowest minimum inhibitory concentration (MIC) values of 1.0 mmol/L (S. typhimurium) and 1.2 mmol/L (Escherichia coli).”
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Metadata
- Authors
- Olasupo, N. A., Fitzgerald, D. J., Gasson, M. J., & Narbad, A.
- Affiliations
- Lagos State University (NG)
- Norwich Research Park (GB)
- Corresponding author
- Narbad, A.
- Venue
- Letters in Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2003-12
- Volume
- 37
- Issue
- 6
- Pages
- 448-451
- ISSN
- 0266-8254 (ISSN-L)
- 1365-2673
- OpenAlex
- W1970033253
- Cited by
- 218
- References
- 22
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 4 · 2023: 7 · 2022: 11 · 2021: 19 · 2020: 11 · 2019: 12 · 2018: 16 · 2017: 13 · 2016: 8 · 2015: 9 · 2014: 10 · 2013: 12 · 2012: 12
- FWCI
- 4.35
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Letters in Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antimicrobial agents and applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- natural antimicrobials, Escherichia coli, Salmonella typhimurium, nisin, thymol, carvacrol, eugenol, cinnamic acid, diacetyl, minimum inhibitory concentration, synergistic and antagonistic effects, Gram-negative bacteria, food safety
- MeSH terms
- Cymenes, Anti-Bacterial Agents, Cinnamates, Diacetyl, Drug Therapy, Combination, Escherichia coli, Eugenol, Microbial Sensitivity Tests, Nisin, Salmonella typhimurium, Thymol, Monoterpenes, In Vitro Techniques
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