The DOI matches its Crossref record; 53 of the 56 references it cites were found in a registry or an index.
Cutaneous Injury Induces the Release of Cathelicidin Anti-Microbial Peptides Active Against Group A Streptococcus
Open accessAbstract
Cathelicidins are a family of peptides thought to provide an innate defensive barrier against a variety of potential microbial pathogens. The human and mouse cathelicidins (LL-37 and CRAMP, respectively) are expressed at select epithelial interfaces where they have been proposed to kill a number of gram-negative and gram-positive bacteria. To determine if these peptides play a part in the protection of skin against wound infections, the anti-microbial activity of LL-37 and CRAMP was determined against the common wound pathogen group A Streptococcus, and their expression was examined after cutaneous injury. We observed a large increase in the expression of cathelicidins in human and murine skin after sterile incision, or in mouse following infection by group A Streptococcus. The appearance of cathelicidins in skin was due to both synthesis within epidermal keratinocytes and deposition from granulocyctes that migrate to the site of injury. Synthesis and deposition in the wound was accompanied by processing from the inactive prostorage form to the mature C-terminal peptide. Analysis of anti-microbial activity of this C-terminal peptide against group A Streptococcus revealed that both LL-37 and CRAMP potently inhibited bacterial growth. Action against group A Streptococcus occurred in conditions that typically abolish the activity of anti-microbial peptides against other organisms. Thus, cathelicidins are well suited to provide defense against infections due to group A Streptococcus, and represent an important element of cutaneous innate immunity.
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“Early events in the innate immune response to skin injury include the recruitment of neutrophils and the induction of antimicrobial peptides, particularly, in humans, the cathelicidin LL-37.17”
From The Innate Immune System in Acute and Chronic Wounds (2016) · cites this paper as [17]
In the citing paper
“Interestingly, suction blister wounds, where the blister roof is still intact, do not show substantial inflammation,9 whereas excisional wounds, ultraviolet (UV)-induced skin damage, superficial tape stripping or chemical skin irritants, and contact sensitizers cause inflammatory infiltrates,17,23–26”
From The Innate Immune System in Acute and Chronic Wounds (2016) · cites this paper as [17]
Citations
Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,515
Manual of Clinical Microbiology
Klainer, A. (1970) · Postgraduate Medical Journal · doi:10.1136/pgmj.46.542.733-c · Cited by 5,344
Zasloff, M. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.15.5449 · Cited by 2,305
Manual of Clinical Microbiology, 7th ed.
Balows, A. (2000) · Diagnostic Microbiology and Infectious Disease · doi:10.1016/s0732-8893(99)00102-9 · Cited by 1,613
Defensins. Natural peptide antibiotics of human neutrophils.
Ganz, T., Selsted, M. E., Szklarek, D., Harwig, S. S. L., Daher, K. A., Bainton, D. F., & Lehrer, R. I. (1985) · Journal of Clinical Investigation · doi:10.1172/jci112120 · Cited by 1,416
A peptide antibiotic from human skin
Harder, J., Bartels, J., Christophers, E., & Schröder, J. ‐. (1997) · Nature · doi:10.1038/43088 · Cited by 1,365
Human β-Defensin-1 Is a Salt-Sensitive Antibiotic in Lung That Is Inactivated in Cystic Fibrosis
Goldman, M. J., Anderson, G. M., Stolzenberg, E. D., Kari, U. P., ZASLOFF, M. A., & Wilson, J. M. (1997) · Cell · doi:10.1016/s0092-8674(00)81895-4 · Cited by 1,077
Defensins: Antimicrobial and Cytotoxic Peptides of Mammalian Cells
Lehrer, R. I., Lichtenstein, A. K., & Ganz, T. (1993) · Annual Review of Immunology · doi:10.1146/annurev.iy.11.040193.000541 · Cited by 1,000
Frohm, M., Agerberth, B., Ahangari, G., Ståhle‐Bäckdahl, M., Lidén, S., Wigzell, H., & Gudmundsson, G. H. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.24.15258 · Cited by 803
Self-citationActivities of LL-37, a Cathelin-Associated Antimicrobial Peptide of Human Neutrophils
Turner, J., Cho, Y., Dinh, N.-N., Waring, A. J., & Lehrer, R. I. (1998) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.42.9.2206 · Cited by 800
Bals, R., Wang, X., ZASLOFF, M. A., & Wilson, J. M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.16.9541 · Cited by 745
Zanetti, M., Gennaro, R., & Romeo, D. (1995) · FEBS Letters · doi:10.1016/0014-5793(95)01050-o · Cited by 708
Conformation-dependent Antibacterial Activity of the Naturally Occurring Human Peptide LL-37
Johansson, J., Gudmundsson, G. H., Rottenberg, M. E., Berndt, K. D., & Agerberth, B. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.6.3718 · Cited by 662
Self-citationGudmundsson, G. H., Agerberth, B., Odeberg, J., Bergman, T., Olsson, B., & Salcedo, R. (1996) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1996.0325z.x · Cited by 579
Self-citationIndolicidin, a novel bactericidal tridecapeptide amide from neutrophils.
Selsted, M. E., Novotny, M., Morris, W. L., Tang, Y. Q., Smith, W. N., & Cullor, J. S. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)42830-x · Cited by 548
Paneth cells of the human small intestine express an antimicrobial peptide gene
Jones, D. E., & Bevins, C. L. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)50079-x · Cited by 537
Agerberth, B., Gunne, H., Odeberg, J., Kogner, P., Boman, H. G., & Gudmundsson, G. H. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.1.195 · Cited by 502
Self-citationGallo, R. L., Kim, K. J., Bernfield, M. R., Kozak, C. A., Zanetti, M., Merluzzi, L., & Gennaro, R. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.20.13088 · Cited by 419
Self-citationBactericidal Activity of Mammalian Cathelicidin-Derived Peptides
Travis, S. M., Anderson, N. N., Forsyth, W. R., Espiritu, C., Conway, B. D., Greenberg, E. P., McCray, P. B., Lehrer, R. I., Welsh, M. J., & Tack, B. F. (2000) · Infection and Immunity · doi:10.1128/iai.68.5.2748-2755.2000 · Cited by 369
Sørensen, O. E., Arnljots, K., Cowland, J. B., Bainton, D. F., & Borregaard, N. (1997) · Blood · doi:10.1182/blood.v90.7.2796 · Cited by 368
Gallo, R. L., Ono, M., Povsic, T. J., Page, C., Eriksson, E. K., Klagsbrun, M., & Bernfield, M. R. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.23.11035 · Cited by 361
Self-citationAgerberth, B., Jong‐Youn, L. E. E., Bergman, T., Carlquist, M., Boman, H. G., Mutt, V., & Jörnvall, H. (1991) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1991.tb16442.x · Cited by 348
Self-citationInvasion of brain microvascular endothelial cells by group B streptococci
Nizet, V., Kim, K. S., Stins, M. F., Jonas, M., Chi, E. Y., Nguyen, D., & Rubens, C. E. (1997) · Infection and Immunity · doi:10.1128/iai.65.12.5074-5081.1997 · Cited by 300
Self-citationGene‐Encoded Peptide Antibiotics and the Concept of Innate Immunity: An Update Review
Boman (1998) · Scandinavian Journal of Immunology · doi:10.1046/j.1365-3083.1998.00343.x · Cited by 222
Epithelial antibiotic induced in states of disease
Stolzenberg, E. D., Anderson, G. M., Ackermann, M. R., Whitlock, R. H., & ZASLOFF, M. A. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.16.8686 · Cited by 219
Biochemical and Antibacterial Analysis of Human Wound and Blister Fluid
Frohm, M., Gunne, H., Bergman, A., Agerberth, B., Bergman, T., Boman, A., Lidén, S., Jörnvall, H., & Boman, H. G. (1996) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1996.0086n.x · Cited by 212
Self-citationAntibiotic peptides from higher eukaryotes: biology and applications
Ganz, T., & Lehrer, R. I. (1999) · Molecular Medicine Today · doi:10.1016/s1357-4310(99)01490-2 · Cited by 197
Group B streptococcal beta-hemolysin expression is associated with injury of lung epithelial cells
Nizet, V., Gibson, R. L., Chi, E. Y., Framson, P. E., Hulse, M., & Rubens, C. E. (1996) · Infection and Immunity · doi:10.1128/iai.64.9.3818-3826.1996 · Cited by 192
Self-citationShi, J. N., Ross, C. R., Leto, T. L., & Blecha, F. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.12.6014 · Cited by 185
Antimicrobial Peptides: An Emerging Concept in Cutaneous Biology
Gallo, R. L., & Huttner, K. M. (1998) · Journal of Investigative Dermatology · doi:10.1046/j.1523-1747.1998.00361.x · Cited by 180
Self-citationGenetic Correlates of Throat and Skin Isolates of Group A Streptococci
Bessen, D. E., Sotir, C. M., Readdy, T. L., & Hollingshead, S. K. (1996) · The Journal of Infectious Diseases · doi:10.1093/infdis/173.4.896 · Cited by 180
Neutrophil antibacterial peptides, multifunctional effector molecules in the mammalian immune system
Gudmundsson, G. H., & Agerberth, B. (1999) · Journal of Immunological Methods · doi:10.1016/s0022-1759(99)00152-0 · Cited by 174
Self-citationAgerberth, B., Grünewald, J., Castaños‐Vélez, E., OLSSON, B., Jörnvall, H., Wigzell, H., Eklúnd, A., & Gudmundsson, G. H. (1999) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.160.1.9807041 · Cited by 165
Self-citationCytotoxicity and Apoptosis Mediated by Two Peptides of Innate Immunity
Risso, A., Zanetti, M., & Gennaro, R. (1998) · Cellular Immunology · doi:10.1006/cimm.1998.1358 · Cited by 146
Zanetti, M., Litteri, L., Gennaro, R., Horstmann, H., & Romeo, D. (1990) · The Journal of Cell Biology · doi:10.1083/jcb.111.4.1363 · Cited by 146
A novel granulocyte-derived peptide with lipopolysaccharide-neutralizing activity.
Larrick, J. W., Hirata, M., Zheng, H., Zhong, J., Bolin, D., Cavaillon, J., Warren, H. S., & Wright, S. C. (1994) · The Journal of Immunology · doi:10.4049/jimmunol.152.1.231 · Cited by 142
Reduced Virulence of Group A Streptococcal Tn 916 Mutants That Do Not Produce Streptolysin S
Betschel, S., Borgia, S. M., Barg, N. L., Low, D. E., & de Azavedo, J. C. S. (1998) · Infection and Immunity · doi:10.1128/iai.66.4.1671-1679.1998 · Cited by 142
Apolipoprotein A-I Binds and Inhibits the Human Antibacterial/Cytotoxic Peptide LL-37
Wang, Y., Agerberth, B., Löthgren, A., Almstedt, A., & Johansson, J. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.50.33115 · Cited by 134
Self-citationChemoattractant properties of PR-39, a neutrophil antibacterial peptide
Huang, H.-J., Ross, C. R., & Blecha, F. (1997) · Journal of Leukocyte Biology · doi:10.1002/jlb.61.5.624 · Cited by 133
Localization and genomic organization of sheep antimicrobial peptide genes
Huttner, K. M., Lambeth, M. R., Burkin, H. R., Burkin, D. J., & Broad, T. E. (1998) · Gene · doi:10.1016/s0378-1119(97)00569-6 · Cited by 118
Electrotransformation of Streptococcus pyogenes with plasmid and linear DNA
Simon, D. A. (1991) · FEMS Microbiology Letters · doi:10.1016/0378-1097(91)90336-9 · Cited by 109
Structural organization of the bovine cathelicidin gene family and identification of a novel member1
Scocchi, M., Wang, S., & Zanetti, M. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)01310-0 · Cited by 94
Electrotransformation ofStreptococcus pyogeneswith plasmid and linear DNA
Simon, D. A., & Ferretti, J. J. (1991) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1991.tb04868.x · Cited by 93
Novel cathelicidins in horse leukocytes
Scocchi, M., Bontempo, D., Boscolo, S., Tomasinsig, L., Giulotto, E., & Zanetti, M. (1999) · FEBS Letters · doi:10.1016/s0014-5793(99)01097-2 · Cited by 70
Shi, J. N., & Ganz, T. (1998) · Infection and Immunity · doi:10.1128/iai.66.8.3611-3617.1998 · Cited by 69
Shi, J. N., Ross, C. R., Chengappa, M. M., & Blecha, F. (1994) · Journal of Leukocyte Biology · doi:10.1002/jlb.56.6.807 · Cited by 69
Antimicrobial Peptides as Agents of Mucosal Immunity
Bevins, C. L. (2007) · Novartis Foundation symposium · doi:10.1002/9780470514658.ch15 · Cited by 68
Peptide Antibiotics: Holy or Heretic Grails of Innate Immunity?
Boman, H. G. (1996) · Scandinavian Journal of Immunology · doi:10.1046/j.1365-3083.1996.d01-76.x · Cited by 66
Bessen, D. E., & Fischetti, V. A. (1990) · The Journal of Infectious Diseases · doi:10.1093/infdis/161.4.747 · Cited by 62
Korthuis, R. J., Gute, D. C., Blecha, F., & Ross, C. R. (1999) · American Journal of Physiology-Heart and Circulatory Physiology · doi:10.1152/ajpheart.1999.277.3.h1007 · Cited by 48
Cathelicidin Gene Expression in Porcine Tissues: Roles in Ontogeny and Tissue Specificity
Wu, H. J., Zhang, G., Ross, C. R., & Blecha, F. (1999) · Infection and Immunity · doi:10.1128/iai.67.1.439-442.1999 · Cited by 44
Ooi, C. E., Weiss, J. P., Levy, O., & Elsbach, P. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55490-9 · Cited by 39
Wang, Y., Griffiths, W. J., Curstedt, T., & Johansson, J. (1999) · FEBS Letters · doi:10.1016/s0014-5793(99)01363-0 · Cited by 30
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Ong, P. Y., Ohtake, T., Brandt, C., Strickland, I., Boguniewicz, M., Ganz, T., Gallo, R. L., & Leung, D. Y. M. (2002) · New England Journal of Medicine · doi:10.1056/nejmoa021481 · Cited by 1,960
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Lande, R., Gregorio, J., Facchinetti, V., Chatterjee, B., Wang, Y.-H., Homey, B., Cao, W., Wang, Y.-H., Su, B., Nestle, F. O., et al. (2007) · Nature · doi:10.1038/nature06116 · Cited by 1,910
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Pastar, I., Stojadinović, O., Yin, N. C., Ramirez, H. A., Nusbaum, A. G., Sawaya, A. P., Patel, S. B., Khalid, L., Isseroff, R. R., & Tomic‐Canic, M. (2014) · Advances in Wound Care · doi:10.1089/wound.2013.0473 · Cited by 1,604
Metadata
- Authors
- Dorschner, R. A., Pestonjamasp, V. K., Tamakuwala, S., Ohtake, T., Rudisill, J., Nizet, V., Agerberth, B., Gudmundsson, G. H., & Gallo, R. L.
- Affiliations
- University of California San Diego (US)
- Karolinska Institutet (SE)
- Corresponding author
- Gallo, Richard L.
- Venue
- Journal of Investigative Dermatology
- Publisher
- Elsevier BV
- Published
- 2001-07
- Volume
- 117
- Issue
- 1
- Pages
- 91-97
- ISSN
- 0022-202X (ISSN-L)
- OpenAlex
- W2085793178
- Cited by
- 567
- References
- 56
- Citations by year
- 2026: 8 · 2025: 10 · 2024: 13 · 2023: 17 · 2022: 11 · 2021: 12 · 2020: 16 · 2019: 25 · 2018: 23 · 2017: 24 · 2016: 20 · 2015: 26 · 2014: 25 · 2013: 23 · 2012: 33
- FWCI
- 14.20
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Investigative Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (AI48176, R21AI048176)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR-44379)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Health Sciences › Medicine › Infectious Diseases › Streptococcal Infections and Treatments
- Health Sciences › Medicine › Surgery › Wound Healing and Treatments
- Keywords
- cathelicidin, LL-37, cramps, Streptococcus pyogenes, skin wounds, wound infection, antimicrobial peptides, innate immunity, keratinocytes, granulocytes, skin
- MeSH terms
- Amino Acid Sequence, Animals, Female, Humans, Male, Mice, Inbred C57BL, Molecular Sequence Data, Proteins, RNA, Messenger, Skin, Streptococcal Infections, Streptococcus pyogenes, Wound Healing, Keratinocytes, Gene Expression, Antimicrobial Cationic Peptides, Mice, Cathelicidins
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