CoChat
Sign Up
Cited by 302Spectroscopy Techniques in Biomedical and Chemical ResearchSpectroscopy and Chemometric AnalysesMarine Plankton and Biogeochemistry

The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.

FOURIER TRANSFORM INFRARED SPECTROSCOPY AS A NOVEL TOOL TO INVESTIGATE CHANGES IN INTRACELLULAR MACROMOLECULAR POOLS IN THE MARINE MICROALGA CHAETOCEROS MUELLERII (BACILLARIOPHYCEAE)

Giordano, Mario, Kansiz, Mustafa, Heraud, Philip, Beardall, John, Wood, Bayden, McNaughton, Don·2001·Journal of Phycology
DOI 10.1046/j.1529-8817.2001.037002271.x
Open this paper in CoChat — free account

Abstract

Fourier Transform Infrared (FT‐IR) spectroscopy was used to study carbon allocation patterns in response to changes in nitrogen availability in the diatom Chaetoceros muellerii Lemmerman. The results of the FT‐IR measurements were compared with those obtained with traditional chemical methods. The data obtained with both FT‐IR and chemical methods showed that nitrogen starvation led to the disappearance of the differences in cell constituents and growth rates existing between cells cultured at either high [NO 3 − ] or high [NH 4 + ]. Irrespective of the nitrogen source supplied before nitrogen starvation, a diversion of carbon away from protein, chlorophyll, and carbohydrates into lipids was observed. Under these conditions, cells that had previously received nitrogen as nitrate appeared to allocate a larger amount of mobilized carbon into lipids than cells that had been cultured in the presence of ammonia. All these changes were reversed by resupplying the cultures with nitrogen. The rate of protein accumulation in the N‐replete cells was slower than the rate of decrease under nitrogen starvation. This study demonstrates that the relative proportions of the major macromolecules contained in microalgal cells and their changes in response to external stimuli can be determined rapidly, simultaneously, and inexpensively using FT‐IR. The technique proved to be equally reliable to and less labor intensive than more traditional chemical methods.

Overview

Integrity signals

Registry

Crossref · 10.1046/j.1529-8817.2001.037002271.x

Cites

26 of 26 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 26 references

Research works printed but not linked: 6

  1. Dalsass A. 2000.Ruolo della Fosfoenolpiruvato Carbossilasi Nell'assimilazione di Azoto.MA thesis. University of Ancona Ancona Italy 120 pp.
  2. Jeffrey S. W., 1997, Phytoplankton Pigments in Oceanography: Guidelines to Modern Methods
  3. Morris I., 1981, Photosynthetic products, physiological state and phytoplankton growth., Can. Bull. Fish. Aquat. Sci, 210, 83
  4. Pouchard C. J. 1985.Aldrich Library of FT‐IR Spectra. Ed. 1 Vol. 2. Aldrich Chemical Company Inc. Milwaukee WI 1567 pp.
  5. Saavenkova L., 1994, Nitrogen fixation and poly‐3‐hydroxybutyrate accumulation by some Azotobacter genus bacteria strains., Proc. Latv. Acad. Sci. B, 562, 89
  6. 10.1002/(sici)1520-6343(1996)2:3<143::aid-bspy1>3.3.co;2-n · DOI

Cited by

Sorted by citations

Items 201–300 of 302

Related

See all

Metadata

Authors
Giordano, M., Kansiz, M., Heraud, P., Beardall, J., Wood, B., & McNaughton, D.
Affiliations
Monash University (AU)
Corresponding author
Giordano, Mario
ORCID
Venue
Journal of Phycology
Publisher
Wiley
Published
2001-04-18
Volume
37
Issue
2
Pages
271-279
ISSN
  • 0022-3646 (ISSN-L)
  • 1529-8817
DOI
10.1046/j.1529-8817.2001.037002271.x
OpenAlex
W2104207789
Cited by
301
References
26
Citations by year
2026: 5 · 2025: 10 · 2024: 17 · 2023: 6 · 2022: 17 · 2021: 22 · 2020: 21 · 2019: 27 · 2018: 11 · 2017: 25 · 2016: 15 · 2015: 20 · 2014: 12 · 2013: 21 · 2012: 14
FWCI
2.61
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 378 d)
Locations
Journal of Phycology (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
  • Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
  • Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine Plankton and Biogeochemistry
Keywords
diatoms, carbon allocation, nitrogen starvation, nitrogen source, nitrate, ammonium, lipid accumulation, macromolecular composition, protein accumulation, chlorophyll, carbohydrates, FTIR spectroscopy, microalgae growth

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review