Application of Fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions
Abstract
Fourier transform infrared spectroscopy (FT-IR) was applied in algal strain screening and monitoring cell composition dynamics in a marine microalga Isochrysis zhangjiangensis during algal cultivation. The content of lipid, carbohydrate and protein of samples determined by traditional methods had validated the accuracy of FT-IR method. For algal screening, the band absorption ratios of lipid/amide I and carbo/amide I from FT-IR measurements allowed for the selection of Isochrysis sp. and Tetraselmis subcordiformis as the most potential lipid and carbohydrate producers, respectively. The cell composition dynamics of I. zhangjiangensis measured by FT-IR revealed the diversion of carbon allocation from protein to carbohydrate and neutral lipid when nitrogen-replete cells were subjected to nitrogen limitation. The carbo/amide I band absorption ratio had also been demonstrated to depict physiological status under nutrient stress in T. subcordiformis. FT-IR serves as a tool for the simultaneous measurement of lipid, carbohydrate, and protein content in cell.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Off-line FTIR determination of the carbohydrate/amide I ratio can predict photosynthetic efficiency with high accuracy (R2 = 0.998).
“Alternatively, the ratio of carbohydrate/amide I determined by off-line FTIR can be used to predict photosynthetic efficiency with high accuracy (R 2 = 0.998) [27]”
From Microalgal process-monitoring based on high-selectivity spectroscopy tools: status and future perspectives (2018) · cites this paper as [27]
FTIR has been used to measure or infer critical process parameters (CPPs) and critical quality attributes (CQAs) commonly monitored in microalgae reactions, apart from measuring intracellular components.
“Furthermore, apart from measuring intracellular components of microalgae, FTIR has been used to measure or infer a number of critical process parameters (CPPs) and critical quality attributes (CQAs) that are commonly monitored in microalgae reactions.”
From Microalgal process-monitoring based on high-selectivity spectroscopy tools: status and future perspectives (2018) · cites this paper as [27]
FTIR spectroscopy has been used for rapid assessment of lipids without lipid extraction and to monitor changes within intracellular macromolecular pools of microalgae.
“Many of the studies have reported the use of FTIR spectroscopy in the rapid assessment of lipids, without the need for lipid extraction and to monitor the changes within the intracellular macromolecular pools of microalgae [15,20 -23].”
From Response to nutrient variation on lipid productivity in green microalgae captured using second derivative FTIR and Raman spectroscopy (2022) · cites this paper as [21]
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751 · see citing works
Brennan, L., & Owende, P. (2010) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2009.10.009 · Cited by 4,894 · see citing works
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745 · see citing works
Valuable Products from Biotechnology of Microalgae
Pulz, O., & Gross, W. (2004) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-004-1647-x · Cited by 2,132 · see citing works
A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae
Chen, W., Zhang, C., Song, L., Sommerfeld, M., & Hu, Q. (2009) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2009.01.001 · Cited by 681 · see citing works
Dean, A. P., Sigee, D. C., Estrada, B., & Pittman, J. K. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.01.065 · Cited by 523 · see citing works
Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications
Suggett, D. J., Prášil, O., & Borowitzka, M. A. (2010) · Springer eBooks · doi:10.1007/978-90-481-9268-7 · Cited by 486 · see citing works
Fan, J., Andre, C., & Xu, C. (2011) · FEBS Letters · doi:10.1016/j.febslet.2011.05.018 · Cited by 347 · see citing works
Lipid Analysis: Isolation, Separation, Identification and Lipidomic Analysis
Christie, W. W., & Han, X. (2010) · Cited by 319 · see citing works
Stehfest, K., Toepel, J., & Wilhelm, C. (2005) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2005.07.001 · Cited by 316 · see citing works
Giordano, M., Kansiz, M., Heraud, P., Beardall, J., Wood, B., & McNaughton, D. (2001) · Journal of Phycology · doi:10.1046/j.1529-8817.2001.037002271.x · Cited by 301 · see citing works
Li, Y., Han, D., Sommerfeld, M. R., & Hu, Q. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.06.036 · Cited by 294 · see citing works
Fan, J., Yan, C., Andre, C., Shanklin, J., Schwender, J., & Xu, C. (2012) · Plant and Cell Physiology · doi:10.1093/pcp/pcs082 · Cited by 248 · see citing works
Rausch, T. (1981) · Hydrobiologia · doi:10.1007/bf00008520 · Cited by 228 · see citing works
Yao, C., Ai, J., Cao, X., Xue, S., & Zhang, W. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.05.030 · Cited by 213 · see citing works
Feng, D., Chen, Z., Xue, S., & Zhang, W. Y. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.04.006 · Cited by 203 · see citing works
Self-citationMonitoring of biomass composition from microbiological sources by means of FT‐IR spectroscopy
Pistorius, A. M. A., DeGrip, W. J., & Egorova‐Zachernyuk, T. A. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.22220 · Cited by 177 · see citing works
Mutate Chlorella sp. by nuclear irradiation to fix high concentrations of CO2
Cheng, J., Huang, Y., Feng, J., Sun, J., Zhou, J., & Cen, K. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.03.072 · Cited by 166 · see citing works
Laurens, L. M. L., & Wolfrum, E. J. (2011) · BioEnergy Research · doi:10.1007/s12155-010-9098-y · Cited by 159 · see citing works
Multianalyte Serum Analysis Using Mid-Infrared Spectroscopy
Shaw, R. A., Kotowich, S., Leroux, M., & Mantsch, H. H. (1998) · Annals of Clinical Biochemistry International Journal of Laboratory Medicine · doi:10.1177/000456329803500505 · Cited by 149 · see citing works
Fernandes, B. D., Teixeira, J. A., Dragone, G., Vicente, A. A., Kawano, S., Bišová, K., Přibyl, P., Zachleder, V., & Vítová, M. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2013.06.096 · Cited by 140 · see citing works
Heraud, P., Wood, B. R., Tobin, M. J., Beardall, J., & McNaughton, D. (2005) · FEMS Microbiology Letters · doi:10.1016/j.femsle.2005.06.021 · Cited by 123 · see citing works
Somani, B. L., Khanade, J., & Sinha, R. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90172-2 · Cited by 120 · see citing works
Palmucci, M., Ratti, S., & Giordano, M. (2011) · Journal of Phycology · doi:10.1111/j.1529-8817.2011.00963.x · Cited by 104 · see citing works
Sánchez, S., Martı́nez, M., & Espínola, F. (2000) · Biochemical Engineering Journal · doi:10.1016/s1369-703x(00)00071-1 · Cited by 94 · see citing works
Dean, A. P., Nicholson, J. M., & Sigee, D. C. (2008) · European Journal of Phycology · doi:10.1080/09670260801979287 · Cited by 81 · see citing works
Feng, G.-D., Zhang, F., Cheng, L.-H., Xu, X.-H., Zhang, L., & Chen, H.-L. (2013) · Bioresource Technology · doi:10.1016/j.biortech.2012.09.123 · Cited by 74 · see citing works
Jebsen, C., Norici, A., Wagner, H., Palmucci, M., Giordano, M., & Wilhelm, C. (2012) · Physiologia Plantarum · doi:10.1111/j.1399-3054.2012.01636.x · Cited by 69 · see citing works
Lacour, T., Sciandra, A., Talec, A., Mayzaud, P., & Olivier Bernard, A. (2012) · Journal of Phycology · doi:10.1111/j.1529-8817.2012.01154.x · Cited by 53 · see citing works
Microalgae triacylglycerols content by FT-IR spectroscopy
Miglio, R., Palmery, S., Salvalaggio, M., Carnelli, L., Capuano, F., & Borrelli, R. (2013) · Journal of Applied Phycology · doi:10.1007/s10811-013-0007-6 · Cited by 41 · see citing works
Horton, R. B., Duranty, E. R., McConico, M. B., & Vogt, F. (2011) · Applied Spectroscopy · doi:10.1366/10-06122 · Cited by 37 · see citing works
Assessing Nutrient Status of Microalgae Using Chlorophyll a Fluorescence
Shelly, K., Holland, D. P., & Beardall, J. (2010) · Springer eBooks · doi:10.1007/978-90-481-9268-7_11 · Cited by 27 · see citing works
Subcommunity FTIR-spectroscopy to determine physiological cell states
Wagner, H., Dunker, S., Liu, Z., & Wilhelm, C. (2012) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2012.09.008 · Cited by 26 · see citing works
Palmucci, M., & Giordano, M. (2011) · Environmental and Experimental Botany · doi:10.1016/j.envexpbot.2011.07.005 · Cited by 22 · see citing works
HPLC Separation of Acyl Lipid Classes
Lin, J. (2007) · Journal of Liquid Chromatography & Related Technologies · doi:10.1080/10826070701435020 · Cited by 18 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Grace, C. E. E., Lakshmi, P. K., Meenakshi, S., Vaidyanathan, S., Srisudha, S., & Mary, M. B. (2020) · Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2019.117382 · Cited by 104 · see citing works
Algal Toxicity Induced by Effluents from Textile-Dyeing Wastewater Treatment Plants
Cai, H., Liang, J., Ning, X.-A., Lai, X., & Li, Y. (2020) · Journal of Environmental Sciences · doi:10.1016/j.jes.2020.01.004 · Cited by 80 · see citing works
Ferro, L., Gojkovic, Z., Gorzsás, A., & Funk, C. (2019) · Molecules · doi:10.3390/molecules24183237 · Cited by 65 · see citing works
Bajhaiya, A. K., Dean, A. P., Driver, T., Trivedi, D. K., Rattray, N. J. W., Allwood, J. W., Goodacre, R., & Pittman, J. K. (2016) · Metabolomics · doi:10.1007/s11306-015-0878-4 · Cited by 49 · see citing works
Podevin, M., Fotidis, I. A., & Angelidaki, I. (2018) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2017.1398132 · Cited by 34 · see citing works
Yaakob, M. A., Mohamed, R. M. S. R., Al-Gheethi, A., Tiey, A., & Kassim, A. H. M. (2019) · Environmental Science and Pollution Research · doi:10.1007/s11356-019-04633-0 · Cited by 29 · see citing works
Advances in techniques for assessment of microalgal lipids
Challagulla, V., Nayar, S., Walsh, K., & Fabbro, L. (2017) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2016.1206058 · Cited by 27 · see citing works
Esther Elizabeth Grace, C., Briget Mary, M., Vaidyanathan, S., & Srisudha, S. (2022) · Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy · doi:10.1016/j.saa.2021.120830 · Cited by 21 · see citing works
Surveillance of C-Allocation in Microalgal Cells
Wagner, H., Jungandreas, A., Fanesi, A., & Wilhelm, C. (2014) · Metabolites · doi:10.3390/metabo4020453 · Cited by 16 · see citing works
Methods for quantitative determination of microalgal lipid and fatty acids content
Morshchinin, I. V. (2025) · Food systems · doi:10.21323/2618-9771-2025-8-2-267-275 · Cited by 3 · see citing works
- Authors
- Meng, Y., Yao, C., Xue, S., & Yang, H.
- Affiliations
- Dalian Institute of Chemical Physics (CN)
- Dalian University (CN)
- Corresponding author
- Xue, Song
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2014-01
- Volume
- 151
- Pages
- 347-354
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W1985399585
- Cited by
- 174
- References
- 36
- Citations by year
- 2026: 11 · 2025: 13 · 2024: 11 · 2023: 16 · 2022: 21 · 2021: 17 · 2020: 21 · 2019: 16 · 2018: 15 · 2017: 8 · 2016: 13 · 2015: 7 · 2014: 4
- FWCI
- 2.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Dalian Institute of Chemical Physics, CAS Institutional Repository (submittedVersion)
- Funders
- Hundred Talent Program of the Chinese Academy of Sciences (A1097)
- Hi-Tech Research and Development Program of China “863 program” (2012AA052101)
- National Key Basic Research Program of China “973 Program” (2011CB200903)
- Liaoning Provincial Natural Science Foundation (2012010263)
- Knowledge Innovation Programs of Dalian Institute of Chemical Physics
- Chinese Academy of Sciences (K2010A13)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Keywords
- FTIR spectroscopy, Tetraselmis subcordiformis, lipid content, carbohydrate content, protein content, nitrogen limitation, carbon allocation, nutritional stress
- MeSH terms
- Carbohydrates, Lipids, Proteins, Stress, Physiological, Time Factors, Spectroscopy, Fourier Transform Infrared, Biomass, Microalgae
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
