Mapping of nutrient-induced biochemical changes in living algal cells using synchrotron infrared microspectroscopy
Abstract
High quality Fourier transform infrared (FTIR) spectra were acquired from living Micrasterias hardyi cells maintained in an IR transparent flow-through cell using a FTIR microscope coupled to a synchrotron light source. Spectral maps of living, nutrient-replete cells showed band intensities consistent with the known location of the nucleus and the chloroplasts. These were very similar to maps acquired from fixed, air-dried cells. Bands due to lipids were lowest in absorbance in the region of the nucleus and highest in the chloroplast region and this trend was reversed for the absorbance of bands attributed to protein. Spectra acquired in 10 microm steps across living phosphorus-starved (P-starved) cells, repeated approximately every 30 min, were consistent over time, and bands correlated well with the known position of the nucleus and the observed chloroplasts, corroborating the observations with replete cells. Experiments in which missing nutrients were re-supplied to starved cells showed that cells could be maintained in a functional state in the flow-through cell for up to one day. Nitrogen-starved cells re-supplied with N showed an increase in lipid in all positions measured across the cell over a 23 h period of re-supply, with the largest increases occurring in positions where the chloroplasts were observed. Re-supply of phosphorus to P-starved cells produced no changes in bands attributable to lipid or protein. Due to their thin cell body ( approximately 12 microm) and large diameter ( approximately 300 microm) Micrasterias sp. make an ideal spectroscopic model to study nutrient kinetics in algal cells.
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Unlike ATR-FTIR, micro-FTIR is still limited from water interferences; however, microscopically focused radiation and high spatial resolution detectors minimize the interference of water.
“Unlike ATR-FTIR, micro-FTIR is still limited from water interferences; however, microscopically focused radiation and high spatial resolution detectors minimize the interference of water [63].”
From Microalgal process-monitoring based on high-selectivity spectroscopy tools: status and future perspectives (2018) · cites this paper as [63]
The Results and discussion section (page not specified) identifies water absorbance as a concern: the authors chose the amide II band because water absorbance is lower there than at amide I. The FTIR data collection section describes focusing synchrotron radiation on a selected cell region, but the paper does not say that high-spatial-resolution detectors minimize water interference or compare micro-FTIR with ATR-FTIR. (LLM, conf=0.83)
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- Authors
- Heraud, P., Wood, B. R., Tobin, M. J., Beardall, J., & McNaughton, D.
- Affiliations
- Monash University (AU)
- Daresbury Laboratory (GB)
- Corresponding author
- Heraud, Philip
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2005-08
- Volume
- 249
- Issue
- 2
- Pages
- 219-225
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W2028141097
- Cited by
- 123
- References
- 26
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 3 · 2021: 8 · 2020: 4 · 2019: 5 · 2018: 6 · 2017: 9 · 2016: 5 · 2015: 10 · 2014: 9 · 2013: 9 · 2012: 8
- FWCI
- 2.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEMS Microbiology Letters (publishedVersion)
- PubMed (publishedVersion)
- Science and Technology Facilities Council (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- nutrient deprivation, phosphorus deficiency, nitrogen starvation, lipid accumulation, protein content, chloroplasts, nucleus, synchrotron infrared microspectroscopy, flow cell
- MeSH terms
- Proteins, Spectrophotometry, Infrared, Synchrotrons, Spectroscopy, Fourier Transform Infrared, Lipid Metabolism, Eukaryota
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