Cited by 55Mitochondrial Function and PathologyAlcohol Consumption and Health EffectsDrug-Induced Hepatotoxicity and Protection

Chronic alcohol consumption increases the sensitivity of rat liver mitochondrial respiration to inhibition by nitric oxide

Venkatraman, Aparna, Shiva, Sruti, Davis, Ashley J., Bailey, Shannon M., Brookes, Paul S., Darley-Usmar, Victor M.·2003·Hepatology

Abstract

Chronic alcohol consumption is a well-known risk factor for hepatic injury, and mitochondrial damage plays a significant role in this process. Nitric oxide (NO) is an important modulator of mitochondrial function and is known to inhibit mitochondrial respiration. However, the impact of chronic alcohol consumption on NO-dependent control of liver mitochondrial function is unknown. This study examines the effect of alcohol exposure on liver mitochondria in a rat model and explores the interaction of NO and mitochondrial respiration in this context. Mitochondria were isolated from the liver of both control and ethanol-fed rats after 5 to 6 weeks of alcohol consumption. Mitochondria isolated from ethanol-treated rats showed a significant decrease in state 3 respiration and respiratory control ratio that was accompanied by an increased sensitivity to NO-dependent inhibition of respiration. In conclusion, we show that chronic alcohol consumption leads to increased sensitivity to the inhibition of respiration by NO. We propose that this results in a greater vulnerability to hypoxia and the development of alcohol-induced hepatotoxicity.

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Crossref · 10.1053/jhep.2003.50293

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Authors
Venkatraman, A., Shiva, S., Davis, A. J., Bailey, S. M., Brookes, P. S., & Darley-Usmar, V. M.
Affiliations
University of Alabama at Birmingham (US)
Corresponding author
Darley-Usmar, Victor M.
Venue
Hepatology
Published
2003-07
Volume
38
Issue
1
Pages
141-147
ISSN
0270-9139 (ISSN-L)
OpenAlex
W2034976767
Cited by
55
References
46
Citations by year
2024: 1 · 2023: 1 · 2022: 3 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 7 · 2012: 2
FWCI
1.49
Type
journal article
Language
en
Open access
closed
Locations
  • Hepatology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r21aa013682-03)
  • National Institute on Alcohol Abuse and Alcoholism (AA13682, R01AA013395, AA-13395)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Alcohol Consumption and Health Effects
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
Keywords
chronic alcohol consumption, ethanol, nitric oxide, mitochondrial respiration, liver mitochondria, respiratory control ratio, state 3 respiration, ethanol-induced hepatotoxicity, liver injury
MeSH terms
Alcohol Drinking, Ethanol, Animals, Central Nervous System Depressants, Chronic Disease, Liver, Male, Mitochondria, Liver, Nitric Oxide, Oxygen Consumption, Oxyhemoglobins, Tyrosine, Cell Hypoxia, Rats, Sprague-Dawley, Cell Respiration, Rats

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