Chronic alcohol consumption increases the sensitivity of rat liver mitochondrial respiration to inhibition by nitric oxide
Abstract
Chronic alcohol consumption is a well-known risk factor for hepatic injury, and mitochondrial damage plays a significant role in this process. Nitric oxide (NO) is an important modulator of mitochondrial function and is known to inhibit mitochondrial respiration. However, the impact of chronic alcohol consumption on NO-dependent control of liver mitochondrial function is unknown. This study examines the effect of alcohol exposure on liver mitochondria in a rat model and explores the interaction of NO and mitochondrial respiration in this context. Mitochondria were isolated from the liver of both control and ethanol-fed rats after 5 to 6 weeks of alcohol consumption. Mitochondria isolated from ethanol-treated rats showed a significant decrease in state 3 respiration and respiratory control ratio that was accompanied by an increased sensitivity to NO-dependent inhibition of respiration. In conclusion, we show that chronic alcohol consumption leads to increased sensitivity to the inhibition of respiration by NO. We propose that this results in a greater vulnerability to hypoxia and the development of alcohol-induced hepatotoxicity.
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NO-dependent inhibition of mitochondrial respiration was monitored in both AFLD and NAFLD.
“For instance, NO-dependent inhibition of the mitochondrial respiration was monitored in both AFLD and NAFLD [86], [87], [88], [89], [90].”
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Garcı́a-Villafranca, J., Guillén, A., & Castro, J. (2004) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2004.09.004 · Cited by 3 · see citing works
Osborne, B., Brandon, A., Smith, G. C., & Turner, N. (2019) · Mitochondria in Obesity and Type 2 Diabetes · doi:10.1016/b978-0-12-811752-1.00016-x · Cited by 3 · see citing works
Neurochemical Effects of Long Term Consumption of High Fat Diet
Farooqui, A. A. (2015) · Springer eBooks · doi:10.1007/978-3-319-15254-7_2 · Cited by 2 · see citing works
Estrés oxidativo hepatocitario y hepatopatía alcohólica
Vista, P. D. (2008)
Sun, Q. (2013) · D-Scholarship@Pitt (University of Pittsburgh)
- Authors
- Venkatraman, A., Shiva, S., Davis, A. J., Bailey, S. M., Brookes, P. S., & Darley-Usmar, V. M.
- Affiliations
- University of Alabama at Birmingham (US)
- Corresponding author
- Darley-Usmar, Victor M.
- Venue
- Hepatology
- Published
- 2003-07
- Volume
- 38
- Issue
- 1
- Pages
- 141-147
- ISSN
- 0270-9139 (ISSN-L)
- OpenAlex
- W2034976767
- Cited by
- 55
- References
- 46
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 3 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 7 · 2012: 2
- FWCI
- 1.49
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Hepatology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r21aa013682-03)
- National Institute on Alcohol Abuse and Alcoholism (AA13682, R01AA013395, AA-13395)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Pathology and Forensic Medicine › Alcohol Consumption and Health Effects
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
- Keywords
- chronic alcohol consumption, ethanol, nitric oxide, mitochondrial respiration, liver mitochondria, respiratory control ratio, state 3 respiration, ethanol-induced hepatotoxicity, liver injury
- MeSH terms
- Alcohol Drinking, Ethanol, Animals, Central Nervous System Depressants, Chronic Disease, Liver, Male, Mitochondria, Liver, Nitric Oxide, Oxygen Consumption, Oxyhemoglobins, Tyrosine, Cell Hypoxia, Rats, Sprague-Dawley, Cell Respiration, Rats
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