Measurement of mitochondrial respiratory thresholds and the control of respiration by nitric oxide
Abstract
The methods described serve to illustrate not only the importance of measuring NO. thresholds in mitochondria and cells, but also highlight many of the considerations that must be taken when making such measurements. These include the presence of O2, the source of NO., the precise design of the apparatus, and experimental conditions, such as buffer composition, light level, and temperature. When all these factors are considered, accurate interpretations of the role of NO. in controlling mitochondrial function can be made. Such experiments can provide insight into the intricate nature of the interaction between NO. and mitochondria and the variations in this system that can occur under both physiological and pathological conditions.
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UCPs 1-4 are believed to induce an inward proton leak in charged mitochondria.
“A small class of “uncoupling” proteins, called UCPs 1–4, are believed to induce an inward proton “leak” in charged mitochondria (49, 55, 176, 300).”
From Mitochondrial reactive oxygen species production in excitable cells: Modulators of mitochondrial and cell function (2009) · cites this paper as [55]
Up to 25% of basal H+ flux into the matrix could be outside of complex V, as suggested by the heat produced as a fraction of the standard metabolic rate.
“Oxidative phosphorylation is not wholly efficient, as up to 25% of basal H+ flux into the matrix could be outside of complex V, as suggested by the heat produced as a fraction of the standard metabolic rate (Fig. 24) (55).”
From Mitochondrial reactive oxygen species production in excitable cells: Modulators of mitochondrial and cell function (2009) · cites this paper as [55]
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- Authors
- Brookes, P. S., Shiva, S., Patel, R. P., & Darley-Usmar, V. M.
- Affiliations
- University of Alabama (US)
- Venue
- Methods in Enzymology
- Publisher
- Elsevier
- Published
- 2002
- Volume
- 359
- Pages
- 305-319
- ISSN
- 0076-6879 (ISSN-L)
- OpenAlex
- W18874106
- Cited by
- 33
- References
- 15
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 2
- FWCI
- 2.49
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Methods in enzymology on CD-ROM/Methods in enzymology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (1r03aa012613-01)
- National Institute on Alcohol Abuse and Alcoholism (AA 12613)
- National Heart, Lung, and Blood Institute (R01HL058031, HL-58031)
- National Institute of Environmental Health Sciences (R01ES010167, ES-10167)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- nitric oxide, mitochondrial respiration, respiratory control, oxygen, buffer composition, illumination level, temperature, mitochondrial function, experimental apparatus design
- MeSH terms
- Calibration, Electron Transport Complex IV, Electrodes, Mitochondria, Nitric Oxide
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