Screening of Five Essential Oils for Identification of Potential Inhibitors of IL-1-induced Nf-κ B Activation and NO Production in Human Chondrocytes: Characterization of the Inhibitory Activity of α-Pinene
Open accessAbstract
Nuclear factor-kappaB is a key transcription factor activated by pro-inflammatory signals, like interleukin-1beta (IL-1), being required for the expression of many inflammatory and catabolic mediators, such as nitric oxide (NO), that play an important role in arthritic diseases. This work aimed at screening and identifying natural inhibitors of IL-induced NF-kappaB activation and NO production in human articular chondrocytes. Five essential oils obtained from four plants of the Iberian flora, Mentha x piperita L. (Lamiaceae), Origanum virens L. (Lamiaceae), Lavandula luiseri L. (Lamiaceae), and Juniperus oxycedrus L. subsp. oxycedrus (Cupressaceae), were screened for their ability to prevent IL-1-induced NO production. The oil showing higher inhibitory activity was fractionated, concentrated, analyzed for composition elucidation and prepared for further assays. For this purpose, the human chondrocytic cell line C-28/I2 was used to evaluate NF-kappaB activation by determining the cytoplasmic levels of the total and phosphorylated forms of the inhibitory protein, I kappaB-alpha, and the NF-kappaB-DNA binding activity. The essential oil from the leaves of J. oxycedrus in a concentration of 0.02 % (v/v) achieved the greatest inhibition (80 +/- 8%) of IL-1-induced NO production. Chemical analysis showed that this essential oil is predominantly composed of monoterpene hydrocabons, being alpha-pinene [2,6,6-trimethyl-bicyclo(3.1.1)hept-3-ene] the major constituent (76 %). Similarly to the effect of the whole oil, a fraction containing 93% alpha-pinene reduced significantly IL-1-induced I kappaB-alpha degradation. Moreover, alpha-pinene also decreased I kappaB-alpha phosphorylation, NF-kappaB-DNA binding activity, and NO production. Another fraction containing oxygenated mono- and sesquiterpenes was nearly as effective as alpha-pinene. The ability of the alpha-pinene-containing fraction to reduce IL-1-induced NF-kappaB activation and NO production warrants further studies to demonstrate the usefulness of alpha-pinene in the treatment of arthritic diseases and other conditions in which NF-kappaB and NO play pathological roles.
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Juniperus oxycedrus essential oil inhibits IL-1-induced NO production in human chondrocytic cell line C-28/12; the same oil and an α-pinene-rich fraction reduce IL-1-induced IκBα degradation and phosphorylation, NF-κB-DNA binding activity, and NO production.
“The essential oil from the leaves of Juniperus oxycedrus L. of the Iberian flora, mainly constituted by α-pinene, showed a great inhibition of IL-1-induced NO production in the human chondrocytic cell line C-28/12. The same oil and a α-pinene-rich fraction reduced markedly IL-1-induced IκBα degradation and phosphorylation, NF-κB-DNA binding activity and NO production [152].”
From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [152]
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- Authors
- Neves, Â., Rosa, S., Gonçalves, J., Rufino, A., Judas, F., Salgueiro, L., Lopes, M., Cavaleiro, C., & Mendes, A.
- Affiliations
- University of Coimbra (PT)
- Centro de Neurociências e Biologia Celular (PT)
- Unidade Local de Saúde de Coimbra (PT)
- Venue
- Planta Medica
- Publisher
- Georg Thieme Verlag KG
- Published
- 2009-09-11
- Volume
- 76
- Issue
- 03
- Pages
- 303-308
- ISSN
- 0032-0943 (ISSN-L)
- 1439-0221
- OpenAlex
- W2050343693
- Cited by
- 51
- References
- 26
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 8 · 2023: 4 · 2022: 4 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 4 · 2016: 2 · 2015: 3 · 2014: 1 · 2013: 5 · 2012: 4
- FWCI
- 4.24
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- other-oa
- Locations
- Planta Medica (publishedVersion)
- PubMed (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
- Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
- Keywords
- essential oils, α-pinene, NF-κB activation, IL-1β, nitric oxide production, articular chondrocytes, Juniperus oxycedrus, NF-kappaB DNA binding, arthritis, anti-inflammatory activity, monoterpene hydrocarbons, sesquiterpenes, Iberian flora
- MeSH terms
- Bicyclic Monoterpenes, Cell Line, Humans, Interleukin-1, Nitric Oxide, Oils, Volatile, Plant Extracts, NF-kappa B, Juniperus, Lamiaceae, Chondrocytes, Monoterpenes
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