Cited by 51Essential Oils and Antimicrobial ActivityNatural Compounds in Disease TreatmentOsteoarthritis Treatment and Mechanisms

Screening of Five Essential Oils for Identification of Potential Inhibitors of IL-1-induced Nf-κ B Activation and NO Production in Human Chondrocytes: Characterization of the Inhibitory Activity of α-Pinene

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Neves, Ângela, Rosa, Susana, Gonçalves, Juliana, Rufino, Ana, Judas, Fernando, Salgueiro, Lígia, Lopes, Maria, Cavaleiro, Carlos +1 more·2010·Planta Medica

Abstract

Nuclear factor-kappaB is a key transcription factor activated by pro-inflammatory signals, like interleukin-1beta (IL-1), being required for the expression of many inflammatory and catabolic mediators, such as nitric oxide (NO), that play an important role in arthritic diseases. This work aimed at screening and identifying natural inhibitors of IL-induced NF-kappaB activation and NO production in human articular chondrocytes. Five essential oils obtained from four plants of the Iberian flora, Mentha x piperita L. (Lamiaceae), Origanum virens L. (Lamiaceae), Lavandula luiseri L. (Lamiaceae), and Juniperus oxycedrus L. subsp. oxycedrus (Cupressaceae), were screened for their ability to prevent IL-1-induced NO production. The oil showing higher inhibitory activity was fractionated, concentrated, analyzed for composition elucidation and prepared for further assays. For this purpose, the human chondrocytic cell line C-28/I2 was used to evaluate NF-kappaB activation by determining the cytoplasmic levels of the total and phosphorylated forms of the inhibitory protein, I kappaB-alpha, and the NF-kappaB-DNA binding activity. The essential oil from the leaves of J. oxycedrus in a concentration of 0.02 % (v/v) achieved the greatest inhibition (80 +/- 8%) of IL-1-induced NO production. Chemical analysis showed that this essential oil is predominantly composed of monoterpene hydrocabons, being alpha-pinene [2,6,6-trimethyl-bicyclo(3.1.1)hept-3-ene] the major constituent (76 %). Similarly to the effect of the whole oil, a fraction containing 93% alpha-pinene reduced significantly IL-1-induced I kappaB-alpha degradation. Moreover, alpha-pinene also decreased I kappaB-alpha phosphorylation, NF-kappaB-DNA binding activity, and NO production. Another fraction containing oxygenated mono- and sesquiterpenes was nearly as effective as alpha-pinene. The ability of the alpha-pinene-containing fraction to reduce IL-1-induced NF-kappaB activation and NO production warrants further studies to demonstrate the usefulness of alpha-pinene in the treatment of arthritic diseases and other conditions in which NF-kappaB and NO play pathological roles.

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  • Juniperus oxycedrus essential oil inhibits IL-1-induced NO production in human chondrocytic cell line C-28/12; the same oil and an α-pinene-rich fraction reduce IL-1-induced IκBα degradation and phosphorylation, NF-κB-DNA binding activity, and NO production.

    “The essential oil from the leaves of Juniperus oxycedrus L. of the Iberian flora, mainly constituted by α-pinene, showed a great inhibition of IL-1-induced NO production in the human chondrocytic cell line C-28/12. The same oil and a α-pinene-rich fraction reduced markedly IL-1-induced IκBα degradation and phosphorylation, NF-κB-DNA binding activity and NO production [152].”

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Research works printed but not linked: 3

  1. 11 Lo YY, Conquer JA, Grinstein S, Cruz TF . Interleukin-1 β induction of c-fos and collagenase expression in articular chondrocytes: involvement of reactive oxygen species. J Cell Biochem 1998; 69: 19-29.
  2. BMS-345541, blocks both joint inflammation and destruction in collagen-induced arthritis in mice. Arthritis Rheum 2003; 48:2652-2659.
  3. 21 Conseil de l'Europe . Pharmacopée Européene, vol. 1, Maisonneuve S.A., Sainte Ruffine, 1996.

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Authors
Neves, Â., Rosa, S., Gonçalves, J., Rufino, A., Judas, F., Salgueiro, L., Lopes, M., Cavaleiro, C., & Mendes, A.
Affiliations
  • University of Coimbra (PT)
  • Centro de Neurociências e Biologia Celular (PT)
  • Unidade Local de Saúde de Coimbra (PT)
Venue
Planta Medica
Published
2009-09-11
Volume
76
Issue
03
Pages
303-308
ISSN
  • 0032-0943 (ISSN-L)
  • 1439-0221
OpenAlex
W2050343693
Cited by
51
References
26
Citations by year
2026: 2 · 2025: 3 · 2024: 8 · 2023: 4 · 2022: 4 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 4 · 2016: 2 · 2015: 3 · 2014: 1 · 2013: 5 · 2012: 4
FWCI
4.24
Type
journal article
Language
en
Open access
Licence
other-oa
Locations
  • Planta Medica (publishedVersion)
  • PubMed (publishedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, other-oa)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
  • Health Sciences › Medicine › Rheumatology › Osteoarthritis Treatment and Mechanisms
Keywords
essential oils, α-pinene, NF-κB activation, IL-1β, nitric oxide production, articular chondrocytes, Juniperus oxycedrus, NF-kappaB DNA binding, arthritis, anti-inflammatory activity, monoterpene hydrocarbons, sesquiterpenes, Iberian flora
MeSH terms
Bicyclic Monoterpenes, Cell Line, Humans, Interleukin-1, Nitric Oxide, Oils, Volatile, Plant Extracts, NF-kappa B, Juniperus, Lamiaceae, Chondrocytes, Monoterpenes

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