Cited by 2,309Tuberculosis Research and EpidemiologyInfectious Diseases and TuberculosisMycobacterium research and diagnosis

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Rapid Molecular Detection of Tuberculosis and Rifampin Resistance

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Boehme, Catharina C., Nabeta, Pamela, Hillemann, Doris, Nicol, Mark P., Shenai, Shubhada, Krapp, Fiorella, Allen, Jenny, Tahirli, Rasim, Blakemore, Robert, Rustomjee, Roxana, Milovic, Ana, Jones, Martin, O'Brien, Sean M., Persing, David H., Ruesch-Gerdes, Sabine, Gotuzzo, Eduardo, Rodrigues, Camilla, Alland, David, Perkins, Mark D.·2010·New England Journal of Medicine

DOI 10.1056/nejmoa0907847PMC PMC2947799

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Abstract

BACKGROUND: Global control of tuberculosis is hampered by slow, insensitive diagnostic methods, particularly for the detection of drug-resistant forms and in patients with human immunodeficiency virus infection. Early detection is essential to reduce the death rate and interrupt transmission, but the complexity and infrastructure needs of sensitive methods limit their accessibility and effect. METHODS: We assessed the performance of Xpert MTB/RIF, an automated molecular test for Mycobacterium tuberculosis (MTB) and resistance to rifampin (RIF), with fully integrated sample processing in 1730 patients with suspected drug-sensitive or multidrug-resistant pulmonary tuberculosis. Eligible patients in Peru, Azerbaijan, South Africa, and India provided three sputum specimens each. Two specimens were processed with N-acetyl-L-cysteine and sodium hydroxide before microscopy, solid and liquid culture, and the MTB/RIF test, and one specimen was used for direct testing with microscopy and the MTB/RIF test. RESULTS: Among culture-positive patients, a single, direct MTB/RIF test identified 551 of 561 patients with smear-positive tuberculosis (98.2%) and 124 of 171 with smear-negative tuberculosis (72.5%). The test was specific in 604 of 609 patients without tuberculosis (99.2%). Among patients with smear-negative, culture-positive tuberculosis, the addition of a second MTB/RIF test increased sensitivity by 12.6 percentage points and a third by 5.1 percentage points, to a total of 90.2%. As compared with phenotypic drug-susceptibility testing, MTB/RIF testing correctly identified 200 of 205 patients (97.6%) with rifampin-resistant bacteria and 504 of 514 (98.1%) with rifampin-sensitive bacteria. Sequencing resolved all but two cases in favor of the MTB/RIF assay. CONCLUSIONS: The MTB/RIF test provided sensitive detection of tuberculosis and rifampin resistance directly from untreated sputum in less than 2 hours with minimal hands-on time. (Funded by the Foundation for Innovative New Diagnostics.)

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Towards complete and accurate reporting of studies of diagnostic accuracy: The STARD Initiative.
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Towards complete and accurate reporting of studies of diagnostic accuracy: the STARD initiative
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Self-citation
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Urdea, M. S., Penny, L., Olmsted, S. S., Giovanni, M. Y., Kaspar, P., Shepherd, A., Wilson, P. L., Dahl, C. A., Buchsbaum, S., Moeller, G., et al. (2006) · Nature · doi:10.1038/nature05448 · Cited by 589
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Research works printed but not linked: 5

Farmer P, 1998, Int J Tuberc Lung Dis, 2, 869
Cohn ML, 1968, Am Rev Respir Dis, 98, 295
Hillemann D, 2005, Int J Tuberc Lung Dis, 9, 1409
Yagui M, 2006, Int J Tuberc Lung Dis, 10, 838
Urbanczik R, 2009, Int J Tuberc Lung Dis, 13, 799

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The risk of tuberculosis in transplant candidates and recipients: a TBNET consensus statement
Bumbăcea, D., Arend, S. M., Eyüboğlu, F. Ö., Fishman, J. A., Goletti, D., Ison, M. G., Jones, C. E., Kampmann, B., Kotton, C. N., Lange, C. G., et al. (2012) · European Respiratory Journal · doi:10.1183/09031936.00000712 · Cited by 269
Rapid molecular TB diagnosis: evidence, policy making and global implementation of Xpert MTB/RIF
Weyer, K., Mirzayev, F., Migliori, G. B., Van Gemert, W., D'Ambrosio, L., Zignol, M., Floyd, K., Centis, R., Cirillo, D. M., Tortoli, E., et al. (2013) · European Respiratory Journal · doi:10.1183/09031936.00157212 · Cited by 256
Evaluation of the Cepheid Xpert MTB/RIF Assay for Direct Detection of Mycobacterium tuberculosis Complex in Respiratory Specimens
Marlowe, E. M., Novak-Weekley, S. M., Cumpio, J., Sharp, S. E., Momeny, M. A., Babst, A., Carlson, J. S., Kawamura, M., & Pandori, M. (2011) · Journal of Clinical Microbiology · doi:10.1128/jcm.02214-10 · Cited by 254
Xpert® MTB/RIF assay for pulmonary tuberculosis and rifampicin resistance in adults
Steingart, K. R., Sohn, H., Schiller, I., Kloda, L. A., Boehme, C. C., Pai, M., & Dendukuri, N. (2013) · Cochrane Database of Systematic Reviews · doi:10.1002/14651858.cd009593.pub2 · Cited by 253
Self-citation
Drug-resistant tuberculosis: a persistent global health concern
Farhat, M. R., Cox, H. S., Ghanem, M., Denkinger, C. M., Rodrigues, C. S., Aziz, M. S. A. E., Enkh-Amgalan, H., Vambe, D., Ugarte‐Gil, C., Furin, J. J., et al. (2024) · Nature Reviews Microbiology · doi:10.1038/s41579-024-01025-1 · Cited by 261
Self-citation
Global tuberculosis control: lessons learnt and future prospects
Lienhardt, C., Glaziou, P., Uplekar, M. W., Lönnroth, K., Getahun, H., & Raviglione, M. C. (2012) · Nature Reviews Microbiology · doi:10.1038/nrmicro2797 · Cited by 251
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Georghiou, S. B., Magaña, M., Garfein, R. S., Catanzaro, D. G., Catanzaro, A., & Rodwell, T. C. (2012) · PLoS ONE · doi:10.1371/journal.pone.0033275 · Cited by 249
Drug‐resistant tuberculosis: An update on disease burden, diagnosis and treatment
Lange, C. G., Chesov, D., Heyckendorf, J., Leung, C. C., Udwadia, Z. F., & Dheda, K. U. J. (2018) · Respirology · doi:10.1111/resp.13304 · Cited by 245
Evaluation of GeneXpert MTB/RIF for Diagnosis of Tuberculous Meningitis
Nhu, N. T. Q., Heemskerk, D., Thu, D. D. A., Chau, T. T. H., Mai, N. T. H., Nghia, H. D. T., Loc, P. P., Ha, D. T. M., Merson, L., Thinh, T. T. V., et al. (2014) · Journal of Clinical Microbiology · doi:10.1128/jcm.01834-13 · Cited by 244
Point-of-care detection of lipoarabinomannan (LAM) in urine for diagnosis of HIV-associated tuberculosis: a state of the art review
Lawn, S. D. (2012) · BMC Infectious Diseases · doi:10.1186/1471-2334-12-103 · Cited by 243
Origin and Proliferation of Multiple-Drug Resistance in Bacterial Pathogens
Chang, H., Cohen, T., Grad, Y. H., Hanage, W. P., O’Brien, T., & Lipsitch, M. (2015) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00039-14 · Cited by 246
Rifampin Drug Resistance Tests for Tuberculosis: Challenging the Gold Standard
Deun, A. V., Aung, K. J. M., Bola, V., Lebeke, R., Hossain, M. A., de Rijk, W. B., Rigouts, L., Gumusboga, A., Torrea, G., & de Jong, B. C. (2013) · Journal of Clinical Microbiology · doi:10.1128/jcm.00553-13 · Cited by 241
Diagnosis of Pulmonary Tuberculosis: Recent Advances and Diagnostic Algorithms
Ryu, Y. J. (2015) · Tuberculosis & respiratory diseases · doi:10.4046/trd.2015.78.2.64 · Cited by 236
A modular transcriptional signature identifies phenotypic heterogeneity of human tuberculosis infection
Singhania, A., Verma, R., Graham, C. M., Lee, J., Tran, T. A. T., Richardson, M., Lécine, P., Leissner, P., Berry, M. P. R., Wilkinson, R. J., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-04579-w · Cited by 245
Mutations inside rifampicin-resistance determining region of rpoB gene associated with rifampicin-resistance in Mycobacterium tuberculosis
Zaw, M. T., Emran, N. A., & Lin, Z. (2018) · Journal of Infection and Public Health · doi:10.1016/j.jiph.2018.04.005 · Cited by 227
Research for Universal Health Coverage
Dye, C., Reeder, J. C., & Terry, R. F. (2013) · Science Translational Medicine · doi:10.1126/scitranslmed.3006971 · Cited by 241
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Menzies, N. A., Cohen, T., Lin, H.-H., Murray, M. B., & Salomon, J. A. (2012) · PLoS Medicine · doi:10.1371/journal.pmed.1001347 · Cited by 222
HIV-associated opportunistic CNS infections: pathophysiology, diagnosis and treatment
Reoma, L. B., Smith, B., Reich, D. S., Quezado, M., & Nath, A. (2016) · Nature Reviews Neurology · doi:10.1038/nrneurol.2016.149 · Cited by 221
Novel High Sensitivity Tuberculosis Point-of-Care Test for People Living with HIV
Broger, T., Sossen, B., du Toit, E., Kerkhoff, A. D., Schutz, C., Reipold, E. I., Ward, A. M., Barr, D. A., Macé, A., Trollip, A., et al. (2018) · The Lancet Infectious Diseases · doi:10.1016/s1473-3099(19)30001-5 · Cited by 222
Self-citation
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Pulmonary infections in HIV-infected patients: an update in the 21st century
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McNerney, R., & Daley, P. (2011) · Nature Reviews Microbiology · doi:10.1038/nrmicro2521 · Cited by 208
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Migliori, G. B., Jp, Z., Abubakar, I., Ibraim, E., Caminero, J. A., de Vries, G., D’Ambrosio, L., Centis, R., Sotgiu, G., Menegale, O., et al. (2012) · European Respiratory Journal · doi:10.1183/09031936.00203811 · Cited by 208
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Khéchine, A. E., Couderc, C., Flaudrops, C., Raoult, D., & Drancourt, M. (2011) · PLoS ONE · doi:10.1371/journal.pone.0024720 · Cited by 206
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Utility of endobronchial ultrasound-guided transbronchial needle aspiration in patients with tuberculous intrathoracic lymphadenopathy: a multicentre study
Navani, N., Molyneaux, P. L., Breen, R. A. M., Connell, D. W., Jepson, A., Nankivell, M. G., Brown, J. M., Morris-Jones, S. D., Ng, B., Wickremasinghe, M., et al. (2011) · Thorax · doi:10.1136/thoraxjnl-2011-200063 · Cited by 204
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Small, P. M., & Pai, M. (2010) · New England Journal of Medicine · doi:10.1056/nejme1008496 · Cited by 196
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Pai, M., & Schito, M. (2015) · The Journal of Infectious Diseases · doi:10.1093/infdis/jiu803 · Cited by 193
Current Concepts in the Management of Tuberculosis
Sia, I. G., & Wieland, M. L. (2011) · Mayo Clinic Proceedings · doi:10.4065/mcp.2010.0820 · Cited by 193
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McNerney, R., Maeurer, M., Abubakar, I., Marais, B., Mchugh, T. D., Ford, N., Weyer, K., Lawn, S., Grobusch, M. P., Memish, Z., et al. (2012) · The Journal of Infectious Diseases · doi:10.1093/infdis/jir860 · Cited by 191
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Nathanson, E., Nunn, P. P., Uplekar, M. W., Floyd, K., Jaramillo, E., Lönnroth, K., Weil, D. E. C., & Raviglione, M. C. (2010) · New England Journal of Medicine · doi:10.1056/nejmra0908076 · Cited by 187
The heterogeneous evolution of multidrug-resistant Mycobacterium tuberculosis
Müller, B., Borrell, S., Rose, G., & Gagneux, S. (2012) · Trends in Genetics · doi:10.1016/j.tig.2012.11.005 · Cited by 185
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Xie, H., Mire, J., Kong, Y., Chang, M.-H., Hassounah, H. A., Thornton, C. N., Sacchettini, J. C., Cirillo, J. D., & Rao, J. (2012) · Nature Chemistry · doi:10.1038/nchem.1435 · Cited by 183
Xpert Ultra versus Xpert MTB/RIF for pulmonary tuberculosis and rifampicin resistance in adults with presumptive pulmonary tuberculosis
Zifodya, J. S., Kreniske, J. S., Schiller, I., Kohli, M., Dendukuri, N., Schumacher, S. G., Ochodo, E., Haraka, F., Zwerling, A. A., Pai, M., et al. (2021) · Cochrane Database of Systematic Reviews · doi:10.1002/14651858.cd009593.pub5 · Cited by 200
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Friedrich, S. O., Rachow, A., Saathoff, E., Singh, K. P., Mangu, C. D., Dawson, R., Phillips, P. P. J., Venter, A., Bateson, A. L. C., Boehme, C. C., et al. (2013) · The Lancet Respiratory Medicine · doi:10.1016/s2213-2600(13)70119-x · Cited by 181
Self-citation
GWAS for quantitative resistance phenotypes in Mycobacterium tuberculosis reveals resistance genes and regulatory regions
Farhat, M. R., Freschi, L., Calderon, R. I., Ioerger, T. R., Snyder, M. W., Meehan, C. J., de Jong, B. C., Rigouts, L., Sloutsky, A., Kaur, D., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-10110-6 · Cited by 181
Detection of Drug-Resistant Tuberculosis by Xpert MTB/RIF in Swaziland
Sánchez-Padilla, E., Merker, M., Beckert, P., Jochims, F., Dlamini, T., Kahn, P., Bonnet, M., & Niemann, S. (2015) · New England Journal of Medicine · doi:10.1056/nejmc1413930 · Cited by 180
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Armand, S., Vanhuls, P., Delcroix, G., Courcol, R., & Lemaître, N. (2011) · Journal of Clinical Microbiology · doi:10.1128/jcm.02157-10 · Cited by 179
Diagnostic Accuracy of Quantitative PCR (Xpert MTB/RIF) for Tuberculous Meningitis in a High Burden Setting: A Prospective Study
Patel, V. B., Theron, G., Lenders, L., Matinyena, B., Connolly, C., Singh, R., Coovadia, Y., Ndung'u, T., & Dheda, K. (2013) · PLoS Medicine · doi:10.1371/journal.pmed.1001536 · Cited by 179

Authors: Boehme, C. C., Nabeta, P., Hillemann, D., Nicol, M. P., Shenai, S., Krapp, F., Allen, J., Tahirli, R., Blakemore, R., Rustomjee, R., Milovic, A., Jones, M., O'Brien, S. M., Persing, D. H., Ruesch-Gerdes, S., Gotuzzo, E., Rodrigues, C., Alland, D., & Perkins, M. D.
Affiliations: FIND (CH)
Research Center Borstel - Leibniz Lung Center (DE)
National Health Laboratory Service (ZA)
Universidad Peruana Cayetano Heredia (PE)
South African Medical Research Council (ZA)
Rutgers New Jersey Medical School (US)
Cepheid (United States) (US)
ORCID: Boehme, Catharina C.
Nabeta, Pamela
Hillemann, Doris
Nicol, Mark P.
Shenai, Shubhada
Krapp, Fiorella
Milovic, Ana
Gotuzzo, Eduardo
Rodrigues, Camilla
Alland, David
Perkins, Mark D.
Venue: New England Journal of Medicine
Publisher: Massachusetts Medical Society
Published: 2010-09-09
Volume: 363
Issue: 11
Pages: 1005-1015
ISSN: 0028-4793 (ISSN-L)
1533-4406
DOI: 10.1056/nejmoa0907847
PMC: PMC2947799
OpenAlex: W2154326381
Cited by: 2,309
References: 26
Citations by year: 2026: 72 · 2025: 92 · 2024: 70 · 2023: 68 · 2022: 99 · 2021: 128 · 2020: 119 · 2019: 136 · 2018: 154 · 2017: 169 · 2016: 154 · 2015: 176 · 2014: 184 · 2013: 195 · 2012: 226
FWCI: 140.91
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: New England Journal of Medicine (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Rutgers University Community Repository (Rutgers University) (submittedVersion)
Universidad Peruana Cayetano Heredia Institutional Repository (submittedVersion, cc-by-nc-nd)
Funders: National Institute of Allergy and Infectious Diseases (R41AI052523, R01AI080653, R42AI052523, AI52523)
Topics: Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
Health Sciences › Medicine › Infectious Diseases › Infectious Diseases and Tuberculosis
Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
Keywords: Xpert MTB/RIF, tuberculosis diagnosis, rifampicin resistance, multidrug-resistant tuberculosis, Mycobacterium tuberculosis, rapid molecular detection, sputum examination, smear-negative tuberculosis, drug susceptibility testing, HIV coinfection, Peru, Azerbaijan, South Africa, India
MeSH terms: Adolescent, Adult, Aged, Aged, 80 and over, Antitubercular Agents, Bacterial Proteins, Female, Humans, Male, Microbial Sensitivity Tests, Middle Aged, Mycobacterium tuberculosis, Prospective Studies, Reference Standards, Rifampin, DNA-Directed RNA Polymerases, Sensitivity and Specificity, Sputum, Tuberculosis, Polymerase Chain Reaction, Tuberculosis, Multidrug-Resistant, Drug Resistance, Bacterial, Young Adult

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