The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Camalexin is synthesized from indole-3-acetaldoxime, a key branching point between primary and secondary metabolism in Arabidopsis
Open accessAbstract
Characteristic for cruciferous plants is their production of N- and S-containing indole phytoalexins with disease resistance and cancer-preventive properties, previously proposed to be synthesized from indole independently of tryptophan. We show that camalexin, the indole phytoalexin of Arabidopsis thaliana , is synthesized from tryptophan via indole-3-acetaldoxime (IAOx) in a reaction catalyzed by CYP79B2 and CYP79B3. Cyp79B2 / cyp79B3 double knockout mutant is devoid of camalexin, as it is also devoid of indole glucosinolates [Zhao, Y., Hull, A. K., Gupta, N. R., Goss, K. A., Alonso, J., Ecker, J. R., Normanly, J., Chory, J. & Celenza, J. L. (2002) Genes Dev. 16, 3100–3112], and isotope-labeled IAOx is incorporated into camalexin. These results demonstrate that only CYP79B2 and CYP79B3 contribute significantly to the IAOx pool from which camalexin and indole glucosinolates are synthesized. Furthermore, production of camalexin in the sur1 mutant devoid of glucosinolates excludes the possibility that camalexin is derived from indole glucosinolates. CYP79B2 plays an important role in camalexin biosynthesis in that the transcript level of CYP79B2 , but not CYP79B3 , is increased upon induction of camalexin by silver nitrate as evidenced by microarray analysis and promoter–β-glucuronidase data. The structural similarity between cruciferous indole phytoalexins suggests that these compounds are biogenetically related and synthesized from tryptophan via IAOx by CYP79B homologues. The data show that IAOx is a key branching point between several secondary metabolic pathways as well as primary metabolism, where IAOx has been shown to play a critical role in IAA homeostasis.
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A Role for Flavin Monooxygenase-Like Enzymes in Auxin Biosynthesis
Zhao, Y., Christensen, S. K., Fankhauser, C., Cashman, J. R., Cohen, J. D., Weigel, D., & Chory, J. (2001) · Science · doi:10.1126/science.291.5502.306 · Cited by 1,225
Analysis of a Chemical Plant Defense Mechanism in Grasses
Frey, M., Chomet, P., Glawischnig, E., Stettner, C., Grün, S., Winklmair, A., Eisenreich, W., Bacher, A., Meeley, R. B., Briggs, S. P., et al. (1997) · Science · doi:10.1126/science.277.5326.696 · Cited by 694
Self-citationTrp-dependent auxin biosynthesis in Arabidopsis: involvement of cytochrome P450s CYP79B2 and CYP79B3
Zhao, Y., Hull, A. K., Gupta, N. R., Goss, K. A., Alonso, J., Ecker, J. R., Normanly, J., Chory, J., & Celenza, J. L. (2002) · Genes & Development · doi:10.1101/gad.1035402 · Cited by 653
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Plant chemical defense: at what cost?
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Metadata
- Authors
- Glawischnig, E., Hansen, B. G., Olsen, C. E., & Halkier, B. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-05-17
- Volume
- 101
- Issue
- 21
- Pages
- 8245-8250
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2126197960
- Cited by
- 313
- References
- 27
- FWCI
- 6.23
- Type
- journal article
- Language
- en
- Open access
- green
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- Glawischnig, E., Hansen, B. G., Olsen, C. E., & Halkier, B. A. (2004). Camalexin is synthesized from indole-3-acetaldoxime, a key branching point between primary and secondary metabolism in Arabidopsis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0305876101
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