The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Genetic and functional confirmation of the causality of the DGAT1 K232A quantitative trait nucleotide in affecting milk yield and composition
Open accessAbstract
We recently used a positional cloning approach to identify a nonconservative lysine to alanine substitution ( K232A ) in the bovine DGAT1 gene that was proposed to be the causative quantitative trait nucleotide underlying a quantitative trait locus (QTL) affecting milk fat composition, previously mapped to the centromeric end of bovine chromosome 14. We herein generate genetic and functional data that confirm the causality of the DGAT1 K232A mutation. We have constructed a high-density single-nucleotide polymorphism map of the 3.8-centimorgan BULGE30–BULGE9 interval containing the QTL and show that the association with milk fat percentage maximizes at the DGAT1 gene. We provide evidence that the K allele has undergone a selective sweep. By using a baculovirus expression system, we have expressed both DGAT1 alleles in Sf9 cells and show that the K allele, causing an increase in milk fat percentage in the live animal, is characterized by a higher V max in producing triglycerides than the A allele.
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Cites
Detecting recent positive selection in the human genome from haplotype structure
Sabeti, P. C., Reich, D. E., Higgins, J. M., Levine, H. Z. P., Richter, D. J., Schaffner, S. F., Gabriel, S. B., Platko, J. V., Patterson, N. J., McDonald, G. J., et al. (2002) · Nature · doi:10.1038/nature01140 · Cited by 2,106
Finding genes that underlie complex traits.
Glazier, A. M., Nadeau, J. H., & Aitman, T. J. (2002) · Science · doi:10.1126/science.1076641 · Cited by 855
Cited by
Thematic review series: Glycerolipids. DGAT enzymes and triacylglycerol biosynthesis
Yen, C.-L. E., Stone, S. J., Koliwad, S., Harris, C., & Farese, R. V. (2008) · Journal of Lipid Research · doi:10.1194/jlr.r800018-jlr200 · Cited by 1,105
Expression and nutritional regulation of lipogenic genes in the ruminant lactating mammary gland
Bernard, L., Leroux, C., & Chilliard, Y. (2007) · Advances in Experimental Medicine and Biology · doi:10.1007/978-0-387-74087-4_2 · Cited by 249
Genetic and Haplotypic Structure in 14 European and African Cattle Breeds
Gautier, M., Faraut, T., Moazami-Goudarzi, K., Navratil, V., Foglio, M., Grohs, C., Boland, A., Garnier, J.-G., Boichard, D., Lathrop, G. M., et al. (2007) · Genetics · doi:10.1534/genetics.107.075804 · Cited by 158
Mapping, Fine Mapping, and Molecular Dissection of Quantitative Trait Loci in Domestic Animals
Georges, M. (2007) · Annual Review of Genomics and Human Genetics · doi:10.1146/annurev.genom.8.080706.092408 · Cited by 132
Self-citationStructure-Function Analysis of Diacylglycerol Acyltransferase Sequences from 70 Organisms
Cao, H. (2011) · BMC Research Notes · doi:10.1186/1756-0500-4-249 · Cited by 122
Metadata
- Authors
- Grisart, B., Farnir, F., Karim, L., Cambisano, N., Kim, J.-J., Kvasz, A., Mni, M., Simon, P., Frère, J.-M., Coppieters, W., & Georges, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-02-12
- Volume
- 101
- Issue
- 8
- Pages
- 2398-2403
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2098667422
- Cited by
- 447
- References
- 2
- FWCI
- 10.94
- Type
- journal article
- Language
- en
- Open access
- green
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- Grisart, B., Farnir, F., Karim, L., Cambisano, N., Kim, J.-J., Kvasz, A., Mni, M., Simon, P., Frère, J.-M., Coppieters, W., & Georges, M. (2004). Genetic and functional confirmation of the causality of the DGAT1 K232A quantitative trait nucleotide in affecting milk yield and composition. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0308518100
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