Cited by 370Genetic Syndromes and ImprintingReproductive Biology and FertilityBirth, Development, and Health

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Long-term effect of in vitro culture of mouse embryos with serum on mRNA expression of imprinting genes, development, and behavior

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Fernández-Gonzalez, Raúl, Moreira, Pedro, Bilbao, Ainhoa, Jiménez, Adela, Pérez-Crespo, Miriam, Ramírez, Miguel Angel, De Fonseca, Fernando Rodríguez, Pintado, Belén, Gutiérrez-Adán, Alfonso·2004·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.0308560101PMC PMC395892

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Abstract

The long-term developmental and behavioral consequences of mammalian embryo culture are unknown. By altering the culture medium with the addition of FCS, we wanted to determine whether mouse embryos cultured under suboptimal conditions develop aberrant mRNA expression of imprinting genes at the blastocyst stage and whether fetal development, growth, and behavior of adult mice are affected. One-cell embryos obtained from superovulated female B6CBAF 1 mice were cultured for 4 days in K + -modified simplex optimized medium in the presence of either 10% FCS or 1 g/liter BSA. After embryo transfer, born animals were submitted to several developmental and behavior tests. The mRNA expression of some imprinting genes was significantly affected in blastocysts cultured in the presence of FCS. Two of the eight measures of preweaning development and some specific measures of neuromotor development, such as the walking activity, were delayed in the group originated with FCS. After 34 weeks, the weight of female mice cultured in vitro in the presence of FCS was significantly higher than controls. In addition, the locomotion activity of mice was altered at 5 and 15 months. Anatomopathological and histological analysis of animals at 20 months of age showed some large organs and an increase in pathologies. We have found that mice derived from embryos cultured with FCS exhibited specific behavioral alterations in anxiety and displayed deficiencies in implicit memories. Our data indicate that long-term programming of postnatal development, growth, and physiology can be affected irreversibly during the preimplantation period of embryo development by suboptimal in vitro culture.

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Authors: Fernández-Gonzalez, R., Moreira, P., Bilbao, A., Jiménez, A., Pérez-Crespo, M., Ramírez, M. A., De Fonseca, F. R., Pintado, B., & Gutiérrez-Adán, A.
Affiliations: Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Carretera de la Coruña Km 5.9, 28040 Madrid, Spain; and Fundación Hospital Carlos Haya, Avenida Carlos Haya 82, 29010 Málaga, Spain
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (ES)
ORCID: Moreira, Pedro
Bilbao, Ainhoa
Pintado, Belén
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 2004-04-12
Volume: 101
Issue: 16
Pages: 5880-5885
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.0308560101
PMC: PMC395892
OpenAlex: W2147536097
Cited by: 370
References: 47
Citations by year: 2026: 4 · 2025: 4 · 2024: 6 · 2023: 11 · 2022: 8 · 2021: 9 · 2020: 18 · 2019: 12 · 2018: 17 · 2017: 22 · 2016: 16 · 2015: 18 · 2014: 25 · 2013: 11 · 2012: 27
FWCI: 13.57
Type: journal article
Language: en
Open access: green
europepmc.org
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion, cc-by-nc-sa)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Syndromes and Imprinting
Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Birth, Development, and Health
Keywords: embryo culture, fetal bovine serum, genomic imprinting, imprinted genes, blastocyst, preimplantation embryo development, developmental programming, anxiety-like behavior, implicit memory, locomotor activity, growth, organ enlargement
MeSH terms: Animals, Behavior, Animal, Blood, Embryo, Mammalian, Growth, RNA, Messenger, Transcription, Genetic, Genomic Imprinting, Culture Techniques, Mice

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Long-term effect of in vitro culture of mouse embryos with serum on mRNA expression of imprinting genes, development, and behavior

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