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Scanning electron microscopy of cells and tissues under fully hydrated conditions
Open accessAbstract
A capability for scanning electron microscopy of wet biological specimens is presented. A membrane that is transparent to electrons protects the fully hydrated sample from the vacuum. The result is a hybrid technique combining the ease of use and ability to see into cells of optical microscopy with the higher resolution of electron microscopy. The resolution of low-contrast materials is ≈100 nm, whereas in high-contrast materials the resolution can reach 10 nm. Standard immunogold techniques and heavy-metal stains can be applied and viewed in the fluid to improve the contrast. Images present a striking combination of whole-cell morphology with a wealth of internal details. A possibility for direct inspection of tissue slices transpires, imaging only the external layer of cells. Simultaneous imaging with photons excited by the electrons incorporates data on material distribution, indicating a potential for multilabeling and specific scintillating markers.
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Cited by
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Golding, C. G., Lamboo, L. L., Beniac, D. R., & Booth, T. F. (2016) · Scientific Reports · doi:10.1038/srep26516 · Cited by 236
Graphene-enabled electron microscopy and correlated super-resolution microscopy of wet cells
Wojcik, M., Hauser, M., Li, W., Moon, S., & Xu, K. (2015) · Nature Communications · doi:10.1038/ncomms8384 · Cited by 133
Civitarese, A. E., MacLean, P. S., Carling, S., Kerr-Bayles, L., McMillan, R. P., Pierce, A., Becker, T. C., Moro, C., Finlayson, J., Lefort, N., et al. (2010) · Cell Metabolism · doi:10.1016/j.cmet.2010.04.004 · Cited by 95
Modulation of myocellular fat stores: lipid droplet dynamics in health and disease
Meex, R. C. R., Schrauwen, P., & Hesselink, M. K. C. (2009) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.91053.2008 · Cited by 65
Assessment of intramuscular triglycerides: contribution to metabolic abnormalities
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Metadata
- Authors
- Thiberge, S., Nechushtan, A., Sprinzak, D., Gileadi, O., Behar, V., Zik, O., Chowers, Y., Michaeli, S., Schlessinger, J., & Moses, E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-02-26
- Volume
- 101
- Issue
- 10
- Pages
- 3346-3351
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2122390610
- Cited by
- 253
- References
- 0
- FWCI
- 6.26
- Type
- journal article
- Language
- en
- Open access
- green
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- Thiberge, S., Nechushtan, A., Sprinzak, D., Gileadi, O., Behar, V., Zik, O., Chowers, Y., Michaeli, S., Schlessinger, J., & Moses, E. (2004). Scanning electron microscopy of cells and tissues under fully hydrated conditions. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0400088101
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