The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Purification and architecture of the ubiquitous Wave complex
Open accessAbstract
The Wave proteins are major activators of the Arp2/3 complex. The ubiquitous Wave-2 is required for actin polymerization at the leading edge of migrating cells. Here we purify Wave-2 from HeLa cells. Five proteins, Sra, Nap, Wave-2, Abi, and Hspc, are copurified, indicating that they form a tight complex. These proteins are only present in the complexed form, with the exception of Hspc, which displays a free pool. We reconstitute the Wave-2 complex by cotranslating in vitro the five subunits and use this system together with specific immunoprecipitations to study the molecular architecture of the complex. The complex is organized around a core of Nap and Abi. Sra is a peripheral subunit recruited on the Nap side, whereas the Wave and Hspc subunits are recruited on the Abi side of the core.
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Cites
Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck
Eden, S., Rohatgi, R., Podtelejnikov, A. V., Mann, M., & Kirschner, M. W. (2002) · Nature · doi:10.1038/nature00859 · Cited by 835
Self-citationScar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex
Machesky, L. M., Mullins, R. D., Higgs, H. N., Kaiser, D. A., Blanchoin, L., May, R. C., Hall, M. E., & Pollard, T. D. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.7.3739 · Cited by 773
Kunda, P., Craig, G., Dominguez, V., & Baum, B. (2003) · Current Biology · doi:10.1016/j.cub.2003.10.005 · Cited by 341
Suetsugu, S., Miki, H., & Takenawa, T. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.0894 · Cited by 246
p140Sra-1 (specifically Rac1-associated protein) is a novel specific target for Rac1 small GTPase
Kobayashi, K., Kuroda, S., Fukata, M., Nakamura, T., Nagase, T., Nomura, N., Matsuura, Y., Yoshida-Kubomura, N., Iwamatsu, A., & Kaibuchi, K. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.1.291 · Cited by 244
Cited by
The WASP-WAVE protein network: Connecting the membrane to the cytoskeleton.
Takenawa, T., & Suetsugu, S. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2069 · Cited by 947
Regulation of Actin Assembly Associated With Protrusion and Adhesion in Cell Migration
Le Clainche, C., & Carlier, M.-F. (2008) · Physiological Reviews · doi:10.1152/physrev.00021.2007 · Cited by 849
A FAM21-Containing WASH Complex Regulates Retromer-Dependent Sorting
Gomez, T. S., & Billadeau, D. D. (2009) · Developmental Cell · doi:10.1016/j.devcel.2009.09.009 · Cited by 521
Arp2/3 complex interactions and actin network turnover in lamellipodia
Lai, F. P., Szczodrak, M., Block, J., Faix, J., Breitsprecher, D., Mannherz, H. G., Stradal, T. E., Dunn, G. A., Small, J. V., & Rottner, K. (2008) · The EMBO Journal · doi:10.1038/emboj.2008.34 · Cited by 300
Activation of the WAVE Complex by Coincident Signals Controls Actin Assembly
Lebensohn, A. M., & Kirschner, M. W. (2009) · Molecular Cell · doi:10.1016/j.molcel.2009.10.024 · Cited by 263
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Metadata
- Authors
- Gautreau, A., Ho, H.-Y. H., Li, J., Steen, H., Gygi, S. P., & Kirschner, M. W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-03-19
- Volume
- 101
- Issue
- 13
- Pages
- 4379-4383
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2033799675
- Cited by
- 236
- References
- 5
- FWCI
- 7.36
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
