Proteomic mapping provides powerful insights into functional myelin biology
Abstract
Myelin is a dynamic, functionally active membrane necessary for rapid action potential conduction, axon survival, and cytoarchitecture. The number of debilitating neurological disorders that occur when myelin is disrupted emphasizes its importance. Using high-resolution 2D gel electrophoresis, mass spectrometry, and immunoblotting, we have developed an extensive proteomic map of proteins present in myelin, identifying 98 proteins corresponding to at least 130 of the ≈200 spots on the map. This proteomic map has been applied to analyses of the localization and function of selected proteins, providing a powerful tool to investigate the diverse functions of myelin.
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The absence of PMP22 protein in oligodendrocytes is consistent with a previous proteomic study that did not reveal PMP22 in CNS myelin.
“The absence of PMP22 protein in oligodendrocytes is consistent with a previous proteomic study that extensively characterized proteins of CNS myelin and did not reveal PMP22 (Taylor et al., 2004).”
From Identification of Dynamically Regulated MicroRNA and mRNA Networks in Developing Oligodendrocytes (2008) · cites this paper as Taylor et al., 2004
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Cites
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Jellinger, K. A. (2003) · Journal of Neural Transmission. Supplementa · doi:10.1007/978-3-7091-0643-3_7 · Cited by 126 · see citing works
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The Neuropathology of Alcohol-Related Brain Damage
Harper, C. (2009) · Alcohol and Alcoholism · doi:10.1093/alcalc/agn102 · Cited by 420 · see citing works
Harper, C., & Matsumoto, I. (2005) · Current Opinion in Pharmacology · doi:10.1016/j.coph.2004.06.011 · Cited by 342 · see citing works
Identification of Dynamically Regulated MicroRNA and mRNA Networks in Developing Oligodendrocytes
Lau, P., Verrier, J. D., Nielsen, J. A., Johnson, K. R., Notterpek, L., & Hudson, L. D. (2008) · The Journal of Neuroscience · doi:10.1523/jneurosci.1932-08.2008 · Cited by 252 · see citing works
Baer, A. S., Syed, Y. A., Kang, S. U., Mitteregger, D., Vig, R., ffrench-Constant, C., Franklin, R. J. M., Altmann, F., Lubec, G., & Kotter, M. R. (2009) · Brain · doi:10.1093/brain/awn334 · Cited by 211 · see citing works
Swiss, V. A., Nguyen, T., Dugas, J., Ibrahim, A., Barres, B., Androulakis, I. P., & Casaccia, P. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018088 · Cited by 112 · see citing works
- Authors
- Taylor, C. M., Marta, C. B., Claycomb, R. J., Han, D. K., Rasband, M. N., Coetzee, T., & Pfeiffer, S. E.
- Affiliations
- Departments of Neuroscience, MC 3401, and Physiology, MC 3501, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030-3401
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2004-03-19
- Volume
- 101
- Issue
- 13
- Pages
- 4643-4648
- ISSN
- 0027-8424
- 1091-6490
- Cited by
- 102
- References
- 2
- Language
- en
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