The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Exploring the O -GlcNAc proteome: Direct identification of O -GlcNAc-modified proteins from the brain
Open accessAbstract
The covalent modification of intracellular proteins by O -linked β- N -acetylglucosamine ( O -GlcNAc) is emerging as a crucial regulatory posttranslational modification akin to phosphorylation. Numerous studies point to the significance of O -GlcNAc in cellular processes such as nutrient sensing, protein degradation, and gene expression. Despite its importance, the breadth and functional roles of O -GlcNAc are only beginning to be elucidated. Advances in our understanding will require the development of new strategies for the detection and study of O -GlcNAc-modified proteins in vivo . Herein we report the direct, high-throughput analysis of O -GlcNAc-glycosylated proteins from the mammalian brain. The proteins were identified by using a chemoenzymatic approach that exploits an engineered galactosyltransferase enzyme to selectively label O -GlcNAc proteins with a ketone-biotin tag. The tag permits enrichment of low-abundance O -GlcNAc species from complex mixtures and localization of the modification to short amino acid sequences. Using this approach, we discovered 25 O -GlcNAc-glycosylated proteins from the brain, including regulatory proteins associated with gene expression, neuronal signaling, and synaptic plasticity. The functional diversity represented by this set of proteins suggests an expanded role for O -GlcNAc in regulating neuronal function. Moreover, the chemoenzymatic strategy described here should prove valuable for identifying O -GlcNAc-modified proteins in various tissues and facilitate studies of the physiological significance of O -GlcNAc across the proteome.
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Dignam, J. D., Lebovitz, R. M., & Roeder, R. G. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.5.1475 · Cited by 11,260
Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels
Shevchenko, A., Wilm, M., Vorm, O., & Mann, M. (1996) · Analytical Chemistry · doi:10.1021/ac950914h · Cited by 8,995
Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry
Syka, J. E. P., Coon, J. J., Schroeder, M. J., Shabanowitz, J., & Hunt, D. F. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0402700101 · Cited by 2,370
Human Hypertension Caused by Mutations in WNK Kinases
Wilson, F. H., Disse-Nicodème, S., Choate, K. A., Ishikawa, K., Nelson-Williams, C., Desitter, I., Gunel, M., Milford, D. V., Lipkin, G. W., Achard, J.-M., et al. (2001) · Science · doi:10.1126/science.1062844 · Cited by 1,487
Herdegen, T., & Leah, J. D. (1998) · Brain Research Reviews · doi:10.1016/s0165-0173(98)00018-6 · Cited by 1,350
The Neurobiology of Slow Synaptic Transmission
Greengard, P. (2001) · Science · doi:10.1126/science.294.5544.1024 · Cited by 867
Enrichment analysis of phosphorylated proteins as a tool for probing the phosphoproteome
Oda, Y., Nagasu, T., & Chait, B. T. (2001) · Nature Biotechnology · doi:10.1038/86783 · Cited by 777
Binary switches and modification cassettes in histone biology and beyond
Fischle, W., Wang, Y., & David Allis, C. (2003) · Nature · doi:10.1038/nature02017 · Cited by 629
A chemical approach for identifying O -GlcNAc-modified proteins in cells
Vocadlo, D. J., Hang, H. C., Kim, E.-J., Hanover, J. A., & Bertozzi, C. R. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1632821100 · Cited by 538
Review of the in Vivo Functions of the p160 Steroid Receptor Coactivator Family
Xu, J., & Li, Q. (2003) · Molecular Endocrinology · doi:10.1210/me.2003-0116 · Cited by 493
Cited by
Dieterich, D. C., Link, A. J., Graumann, J., Tirrell, D. A., & Schuman, E. M. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0601637103 · Cited by 874
Glycoproteomic probes for fluorescent imaging of fucosylated glycans in vivo
Sawa, M., Hsu, T.-L., Itoh, T., Sugiyama, M., Hanson, S. R., Vogt, P. K., & Wong, C.-H. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0605418103 · Cited by 409
Zhao, Y., & Jensen, O. N. (2009) · PROTEOMICS · doi:10.1002/pmic.200900398 · Cited by 384
Jang, H., Kim, T. W., Yoon, S., Choi, S.-Y., Kang, T.-W., Kim, S.-Y., Kwon, Y.-W., Cho, E.-J., & Youn, H.-D. (2012) · Cell Stem Cell · doi:10.1016/j.stem.2012.03.001 · Cited by 317
Extensive Crosstalk Between O-GlcNAcylation and Phosphorylation Regulates Cytokinesis
Wang, Z., Udeshi, N. D., Slawson, C., Compton, P. D., Sakabe, K., Cheung, W. D., Shabanowitz, J., Hunt, D. F., & Hart, G. W. (2010) · Science Signaling · doi:10.1126/scisignal.2000526 · Cited by 317
Metadata
- Authors
- Khidekel, N., Ficarro, S. B., Peters, E. C., & Hsieh-Wilson, L. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-08-30
- Volume
- 101
- Issue
- 36
- Pages
- 13132-13137
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2149202312
- Cited by
- 299
- References
- 45
- FWCI
- 5.47
- Type
- journal article
- Language
- en
- Open access
- green
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- Khidekel, N., Ficarro, S. B., Peters, E. C., & Hsieh-Wilson, L. C. (2004). Exploring the O -GlcNAc proteome: Direct identification of O -GlcNAc-modified proteins from the brain. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0403471101
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