The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Identification of 315 genes essential for early zebrafish development
Open accessAbstract
We completed a large insertional mutagenesis screen in zebrafish to identify genes essential for embryonic and early larval development. We isolated 525 mutants, representing lesions in ≈390 different genes, and we cloned the majority of these. Here we describe 315 mutants and the corresponding genes. Our data suggest that there are roughly 1,400 embryonic-essential genes in the fish. Thus, we have mutations in ≈25% of these genes and have cloned ≈22% of them. Re-screens of our collection to identify mutants with specific developmental defects suggest that ≈50 genes are essential for the development of some individual organs or cell types. Seventy-two percent of the embryonic-essential fish genes have homologues in yeast, 93% have homologues in invertebrates (fly or worm), and 99% have homologues in human. Yeast and worm orthologues of genes that are essential for early zebrafish development have a strong tendency to be essential for viability in yeast and for embryonic development in the worm. Thus, the trait of being a genetically essential gene is conserved in evolution. This mutant collection should be a valuable resource for diverse studies of cell and developmental biology.
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Metadata
- Authors
- Amsterdam, A., Nissen, R. M., Sun, Z., Swindell, E. C., Farrington, S., & Hopkins, N.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-07-15
- Volume
- 101
- Issue
- 35
- Pages
- 12792-12797
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1975537268
- Cited by
- 800
- References
- 27
- FWCI
- 13.30
- Type
- journal article
- Language
- en
- Open access
- green
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- Amsterdam, A., Nissen, R. M., Sun, Z., Swindell, E. C., Farrington, S., & Hopkins, N. (2004). Identification of 315 genes essential for early zebrafish development. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0403929101
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