The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Mitogen-activated protein kinase pathways defend against bacterial pore-forming toxins
Open accessAbstract
Cytolytic pore-forming toxins are important for the virulence of many disease-causing bacteria. How target cells molecularly respond to these toxins and whether or not they can mount a defense are poorly understood. By using microarrays, we demonstrate that the nematode Caenorhabditis elegans responds robustly to Cry5B, a member of the pore-forming Crystal toxin family made by Bacillus thuringiensis . This genomic response is distinct from that seen with a different stressor, the heavy metal cadmium. A p38 mitogen-activated protein kinase (MAPK) kinase and a c-Jun N-terminal-like MAPK are both transcriptionally up-regulated by Cry5B. Moreover, both MAPK pathways are functionally important because elimination of either leads to animals that are ( i ) hypersensitive to a low, chronic dose of toxin and ( ii ) hypersensitive to a high, brief dose of toxin such that the animal might naturally encounter in the wild. These results extend to mammalian cells because inhibition of p38 results in the hypersensitivity of baby hamster kidney cells to aerolysin, a pore-forming toxin that targets humans. Furthermore, we identify two downstream transcriptional targets of the p38 MAPK pathway, ttm-1 and ttm-2 , that are required for defense against Cry5B. Our data demonstrate that cells defend against pore-forming toxins by means of conserved MAPK pathways.
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Cites
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Systematic functional analysis of the Caenorhabditis elegans genome using RNAi
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C. elegans: des neurones et des gènes
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MAP Kinases in the Immune Response
Dong, C., Davis, R. J., & Flavell, R. A. (2002) · Annual Review of Immunology · doi:10.1146/annurev.immunol.20.091301.131133 · Cited by 1,662
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Loss of the Putative RNA-Directed RNA Polymerase RRF-3 Makes C. elegans Hypersensitive to RNAi
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Bacillus thuringiensis crystal proteins that target nematodes
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Self-citation
Cited by
p38 MAPK Regulates Expression of Immune Response Genes and Contributes to Longevity in C. elegans
Troemel, E. R., Chu, S. W., Reinke, V., Lee, S. S., Ausubel, F. M., & Kim, D. H. (2006) · PLoS Genetics · doi:10.1371/journal.pgen.0020183 · Cited by 678
Gurcel, L., Abrami, L., Girardin, S., Tschopp, J., & van der Goot, F. G. (2006) · Cell · doi:10.1016/j.cell.2006.07.033 · Cited by 508
Self-citationBacterial pore-forming toxins: The (w)hole story?
Gonzalez, M. R., Bischofberger, M., Pernot, L., van der Goot, F. G., & Frêche, B. (2008) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-007-7434-y · Cited by 262
Two-way traffic on the road to plasma membrane repair
Idone, V., Tam, C., & Andrews, N. W. (2008) · Trends in Cell Biology · doi:10.1016/j.tcb.2008.09.001 · Cited by 138
Pore-forming toxins and cellular non-immune defenses (CNIDs)
Aroian, R., & van der Goot, F. (2007) · Current Opinion in Microbiology · doi:10.1016/j.mib.2006.12.008 · Cited by 119
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Metadata
- Authors
- Huffman, D. L., Abrami, L., Sasik, R., Corbeil, J., van der Goot, F. G., & Aroian, R. V.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-07-15
- Volume
- 101
- Issue
- 30
- Pages
- 10995-11000
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2138608045
- Cited by
- 316
- References
- 21
- FWCI
- 5.14
- Type
- journal article
- Language
- en
- Open access
- green
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