CoChat
Sign Up
Cited by 305

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Positive and negative regulation of endogenous genes by designed transcription factors

Beerli, Roger R., Dreier, Birgit, Barbas, Carlos F.·2000·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.040552697
Open this paper in CoChat — free account

Abstract

Gene regulation by imposed localization was studied by using designed zinc finger proteins that bind 18-bp DNA sequences in the 5′ untranslated regions of the protooncogenes erbB-2 and erbB-3 . Transcription factors were generated by fusion of the DNA-binding proteins to repression or activation domains. When introduced into cells these transcription factors acted as dominant repressors or activators of, respectively, endogenous erbB-2 or erbB-3 gene expression. Significantly, imposed regulation of the two genes was highly specific, despite the fact that the transcription factor binding sites targeted in erbB-2 and erbB-3 share 15 of 18 nucleotides. Regulation of erbB-2 gene expression was observed in cells derived from several species that conserve the DNA target sequence. Repression of erbB-2 in SKBR3 breast cancer cells inhibited cell-cycle progression by inducing a G 1 accumulation, suggesting the potential of designed transcription factors for cancer gene therapy. These results demonstrate the willful up- and down-regulation of endogenous genes, and provide an additional means to alter biological systems.

Overview

Integrity signals

Registry

Crossref · 10.1073/pnas.040552697

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 33

  1. 10.1038/324429a0 · DOI
  2. M D Matteucci, R W Wagner Nature (London) 384, 20–22 (1996).
  3. 10.1038/387202a0 · DOI
  4. 10.1016/S0960-9822(07)00508-8 · DOI
  5. 10.1073/pnas.89.16.7345 · DOI
  6. 10.1073/pnas.91.23.11163 · DOI
  7. 10.1126/science.8303274 · DOI
  8. 10.1073/pnas.92.2.344 · DOI
  9. 10.1126/science.275.5300.657 · DOI
  10. 10.1073/pnas.96.6.2758 · DOI · Paper page
  11. 10.1073/pnas.94.11.5525 · DOI · Paper page
  12. 10.1073/pnas.95.25.14628 · DOI
  13. N E Hynes, D F Stern Biochim Biophys Acta 1198, 165–184 (1994).
  14. 10.1073/pnas.90.7.2900 · DOI
  15. M Alimandi, A Romano, M C Curia, R Muraro, P Fedi, S A Aaronson, P P Di Fiore, M H Kraus Oncogene 10, 1813–1821 (1995).
  16. 10.1093/emboj/18.8.2149 · DOI
  17. 10.1016/S0021-9258(18)42160-6 · DOI
  18. 10.1073/pnas.94.20.10669 · DOI
  19. 10.1128/MCB.15.3.1182 · DOI
  20. 10.1073/pnas.96.1.2 · DOI
  21. 10.1016/S0959-440X(99)80007-4 · DOI
  22. 10.1073/pnas.91.10.4509 · DOI
  23. 10.1002/j.1460-2075.1992.tb05603.x · DOI
  24. 10.1073/pnas.86.23.9193 · DOI
  25. 10.1073/pnas.87.13.4905 · DOI
  26. M R White, M C Hung Oncogene 7, 677–683 (1992).
  27. 10.1038/351588a0 · DOI
  28. 10.1073/pnas.90.6.2256 · DOI
  29. 10.1126/science.2885917 · DOI
  30. 10.1128/mcb.9.3.1165-1172.1989 · DOI
  31. J Deshane, F Loechel, R M Conry, G P Siegel, C R King, D T Curiel Gene Ther 1, 332–337 (1994).
  32. J M Daly, C B Jannot, R R Beerli, D Graus-Porta, F G Maurer, N E Hynes Cancer Res 57, 3804–3811 (1997).
  33. Neve R. M. Sutterluty H. Pullen N. Lane H. A. Daly J. M. Krek W. & Hynes N. E. (2000) Oncogene in press.

Cited by

Sorted by citations

See all 305

Metadata

Authors
Beerli, R. R., Dreier, B., & Barbas, C. F.
Affiliations
The Skaggs Institute for Chemical Biology and the Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
2000-01-31
Volume
97
Issue
4
Pages
1495-1500
ISSN
  • 0027-8424
  • 1091-6490
DOI
10.1073/pnas.040552697
Cited by
305
References
2
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review