The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Design of polydactyl zinc-finger proteins for unique addressing within complex genomes
Abstract
Zinc-finger proteins of the Cys 2 -His 2 type represent a class of malleable DNA-binding proteins that may be selected to bind diverse sequences. Typically, zinc-finger proteins containing three zinc-finger domains, like the murine transcription factor Zif268 and the human transcription factor Sp1, bind nine contiguous base pairs. To create a class of proteins that would be generally applicable to target unique sites within complex genomes, we have utilized structure-based modeling to design a polypeptide linker that fuses two three-finger proteins. Two six-fingered proteins were created and demonstrated to bind 18 contiguous bp of DNA in a sequence-specific fashion. Expression of these proteins as fusions to activation or repression domains allows transcription to be specifically up- or down-modulated within human cells. Polydactyl zinc-finger proteins should be broadly applicable as genome-specific transcriptional switches in gene therapy strategies and the development of novel transgenic plants and animals.
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In ZFNs, the FokI cleaving domain is coupled to an assemblage of zinc finger proteins, each recognizing and binding to a triplet of the nucleotide.
“In ZFNs, the FokI cleaving domain is coupled to an assemblage of zinc finger proteins, each recognizing and binding to a triplet of the nucleotide [86,87].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [87]
Citations
Cites
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In vivo repression by a site-specific DNA-binding protein designed against an oncogenic sequence
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Structure-Based Design of Transcription Factors
Pomerantz, J. L., Sharp, P. A., & Pabo, C. O. (1995) · Science · doi:10.1126/science.7809612 · Cited by 226
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ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
Gaj, T., Gersbach, C. A., & Barbas, C. F. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2013.04.004 · Cited by 3,820
Self-citationTargeted Chromosomal Cleavage and Mutagenesis in Drosophila Using Zinc-Finger Nucleases
Bibikova, M., Golic, M., Golic, K. G., & Carroll, D. (2002) · Genetics · doi:10.1093/genetics/161.3.1169 · Cited by 860
Editing the epigenome: technologies for programmable transcription and epigenetic modulation
Thakore, P. I., Black, J. B., Hilton, I. B., & Gersbach, C. A. (2016) · Nature Methods · doi:10.1038/nmeth.3733 · Cited by 451
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Self-citationDesign, engineering, and characterization of zinc finger nucleases
Mani, M., Kandavelou, K., Dy, F. J., Durai, S., & Chandrasegaran, S. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.07.089 · Cited by 129
Loureiro, A., & da Silva, G. J. (2019) · Antibiotics · doi:10.3390/antibiotics8010018 · Cited by 129
Tanenhaus, A., Stowe, T., Young, A., McLaughlin, J., Aeran, R., Lin, I. W., Li, J., Hosur, R., Chen, M., Leedy, J., et al. (2022) · Human Gene Therapy · doi:10.1089/hum.2022.037 · Cited by 124
“1A), 26,28 and was designed to selectively bind a conserved regulatory region upstream of the SCN1A transcription start site (NM_001202435) (see Experimental Procedures section).”
Targeting Serous Epithelial Ovarian Cancer with Designer Zinc Finger Transcription Factors
Lara, H., Wang, Y., Beltran, A. S., Juárez-Moreno, K., Yuan, X., Kato, S., Leisewitz, A. V., Cuello Fredes, M., Licea, A. F., Connolly, D. C., et al. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.360768 · Cited by 42
Metadata
- Authors
- Liu, Q., Segal, D. J., Ghiara, J. B., & Barbas, C. F.
- Affiliations
- The Skaggs Institute for Chemical Biology and the Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1997-05-27
- Volume
- 94
- Issue
- 11
- Pages
- 5525-5530
- ISSN
- 0027-8424
- 1091-6490
- Cited by
- 288
- References
- 3
- Language
- en
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