The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Sperm-specific protein kinase A catalytic subunit Cα 2 orchestrates cAMP signaling for male fertility
Open accessAbstract
An unusual cAMP signaling system mediates many of the events that prepare spermatozoa to meet the egg. Its components include the atypical, bicarbonate-stimulated, sperm adenylyl cyclase and a cAMP-dependent protein kinase (PKA) with the unique catalytic subunit termed Cα 2 or C s . We generated mice that lack Cα 2 to determine its importance in the events downstream of cAMP production. Male Cα 2 null mice produce normal numbers of sperm that swim spontaneously in vitro . Thus, Cα 2 has no required role in formation of a functional flagellum or the initiation of motility. In contrast, we find that Cα 2 is required for bicarbonate to speed the flagellar beat and facilitate Ca 2+ entry channels. In addition, Cα 2 is needed for the protein tyrosine phosphorylation that occurs late in the sequence of sperm maturation and for a negative feedback control of cAMP production, revealed here. Consistent with these specific defects in several important sperm functions, Cα 2 null males are infertile despite normal mating behavior. These results define several crucial roles of PKA in sperm cell biology, bringing together both known and unique PKA-mediated events that are necessary for male fertility.
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Cites
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Cited by
Calcium Channels in the Development, Maturation, and Function of Spermatozoa
Darszon, A., Nishigaki, T., Beltran, C., & Treviño, C. L. (2011) · Physiological Reviews · doi:10.1152/physrev.00028.2010 · Cited by 364
Ion channels, phosphorylation and mammalian sperm capacitation
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Mechanisms of sperm chemotaxis
Kaupp, U. B., Kashikar, N. D., & Weyand, I. (2008) · Annual Review of Physiology · doi:10.1146/annurev.physiol.70.113006.100654 · Cited by 284
Metadata
- Authors
- Nolan, M. A., Babcock, D. F., Wennemuth, G., Brown, W., Burton, K. A., & McKnight, G. S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2004-08-31
- Volume
- 101
- Issue
- 37
- Pages
- 13483-13488
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2071740910
- Cited by
- 259
- References
- 30
- FWCI
- 9.80
- Type
- journal article
- Language
- en
- Open access
- green
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- Nolan, M. A., Babcock, D. F., Wennemuth, G., Brown, W., Burton, K. A., & McKnight, G. S. (2004). Sperm-specific protein kinase A catalytic subunit Cα 2 orchestrates cAMP signaling for male fertility. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0405580101
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